a-MSH, amide
(Synonyms: Ac-Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Val-amide, α-MSH, amide) 目录号 : GP10060
a-MSH(α-黑素细胞刺激素)通过G蛋白转运体与黑色素皮质素-1受体结合并激活环磷酸腺苷(cAMP)信号通路,可以合成黑色素。它是内源性神经肽,是促阿皮黑色素皮质素(POMC)的翻译后衍生物。
Sample solution is provided at 25 µL, 10mM.
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a-MSH (α-Melanocyte-Stimulating Hormone), amide engages the melanocortin-1 receptor and activates cyclic AMP (cAMP) signaling via a G-protein transporter, can synthesize melanin. It is an endogenous neuropeptide, a post-translational derivative of pro-opiomelanocortin (POMC). It is an endogenous melanocortin receptor 4 (MC4R) agonist with anti-inflammatory and antipyretic activities[1-4,10].
α-MSH-treated(1 hour; 300pg/ml) T cells showed higher levels of protein ubiquitination. Resting T cells treated with alpha-MSH also demonstrated enhanced protein ubiquitination[5].In α-MSH-stimulated(1 µM, 72 h) B16F10 cells, autophagosomes engulfing melanosomes following Pt treatment[6]. α-MSH regulates central nervous system inflammation by acting directly on melanocortin receptors in glial cells. α-MSH regulates the activation of NFκB. α-MSH inhibits nuclear transmigration of transcription factor κB[7].
α-MSH(0.5mg/kg;i.p;28days) modulated the excitatory-inhibitory balance in the brain by restoring GABAergic inhibition and improved cognition in TgCRND8 mice[8]. α-MSH(2, 6, 20 or 60 nmol)plays an important regulatory role in the spinal defecation center of rats. Intrathecal administration of α-MSH in mice can enhance the colonic movement of the L6-S1 spinal cord and block the pelvic nerve, but the pro-kinetic effect of α-MSH disappeared[9].
References:
[1]. Kim MS, Rossi M, et,al.Hypothalamic localization of the feeding effect of agouti-related peptide and alpha-melanocyte-stimulating hormone. Diabetes. 2000 Feb;49(2):177-82. doi: 10.2337/diabetes.49.2.177. PMID: 10868932.
[2]. Singh M, Mukhopadhyay K. C-terminal amino acids of alpha-melanocyte-stimulating hormone are requisite for its antibacterial activity against Staphylococcus aureus. Antimicrob Agents Chemother. 2011 May;55(5):1920-9. doi: 10.1128/AAC.00957-10. Epub 2011 Jan 31. PMID: 21282427; PMCID: PMC3088230.
[3]. Catania A, Delgado R, et,al.alpha-MSH in systemic inflammation. Central and peripheral actions. Ann N Y Acad Sci. 1999 Oct 20;885:183-7. doi: 10.1111/j.1749-6632.1999.tb08675.x. PMID: 10816651.
[4]. Anderson EJ, Çakir I, et,al.60 YEARS OF POMC: Regulation of feeding and energy homeostasis by α-MSH. J Mol Endocrinol. 2016 May;56(4):T157-74. doi: 10.1530/JME-16-0014. Epub 2016 Mar 3. PMID: 26939593; PMCID: PMC5027135.
[5]. Biros DJ, Namba K, et,al.Alpha-MSH regulates protein ubiquitination in T cells. Cell Mol Biol (Noisy-le-grand). 2006 May 30;52(2):33-8. PMID: 16914084; PMCID: PMC4698146.
[6]. Hseu YC, Vudhya Gowrisankar Y, et,al. The in vitro and in vivo depigmenting activity of pterostilbene through induction of autophagy in melanocytes and inhibition of UVA-irradiated α-MSH in keratinocytes via Nrf2-mediated antioxidant pathways. Redox Biol. 2021 Aug;44:102007. doi: 10.1016/j.redox.2021.102007. Epub 2021 May 19. PMID: 34049220; PMCID: PMC8167190.
[7]. Lipton JM, Zhao H, et,al.Mechanisms of antiinflammatory action of alpha-MSH peptides. In vivo and in vitro evidence. Ann N Y Acad Sci. 1999 Oct 20;885:173-82. doi: 10.1111/j.1749-6632.1999.tb08674.x. PMID: 10816650.
[8]. Biros DJ, Namba K, et,al.Alpha-MSH regulates protein ubiquitination in T cells. Cell Mol Biol (Noisy-le-grand). 2006 May 30;52(2):33-8. PMID: 16914084; PMCID: PMC4698146.
[9].Ueda HH, Naitou K, et,al. α-MSH-induced activation of spinal MC1R but not MC4R enhances colorectal motility in anaesthetised rats. Sci Rep. 2021 Jan 12;11(1):487. doi: 10.1038/s41598-020-80020-x. PMID: 33436759; PMCID: PMC7803980.
[10].Song, X.; Mosby, N.; Yang, J.; Xu, A.; Abdel-Malek, Z.; Kadekaro, A.L. alpha-MSH activates immediate defense responses to UV-induced oxidative stress in human melanocytes. Pigment Cell Melanoma Res. 2009, 22, 809-818.
a-MSH(α-黑素细胞刺激素)通过G蛋白转运体与黑色素皮质素-1受体结合并激活环磷酸腺苷(cAMP)信号通路,可以合成黑色素。它是内源性神经肽,是促阿皮黑色素皮质素(POMC)的翻译后衍生物。它是内源性黑色素皮质素受体4(MC4R)激动剂,具有抗炎和退热活性[1-4,10]。
α-MSH-treated(1 hour; 300pg/ml) T细胞显示更高水平的蛋白泛素化。α-MSH处理的静止T细胞也表现出增强的蛋白泛素化[5]。在α-MSH刺激(1 µM, 72 h)的B16F10细胞中,Pt处理后自噬体吞噬黑素体[6]。α-MSH通过直接作用于神经胶质细胞的黑素皮质素受体调节中枢神经系统炎症。α-MSH调节NFκB的活化。α-MSH抑制转录因子κB的核迁移[7]。
α-MSH(0.5mg/kg;i.p;28d)通过恢复TgCRND8小鼠中的GABAergic抑制和改善认知来调节大脑兴奋-抑制平衡[8]。α-MSH(2、6、20、60 nmol)在大鼠脊柱排便中枢中起重要的调节作用。小鼠鞘内给予α-MSH可增强L6-S1脊髓的结肠运动,阻断盆腔神经,但α-MSH的促动力学作用消失[9]。
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Protocol for melanogenesis [1]: |
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After heating at 80 ℃ for 1 h, the absorbance at 405 nm was measured using a microplate reader.
This protocol only provides a guideline, and should be modified according to your specific needs. |
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References: [1]. Zhou S, Riadh D, et,al. Grape Extract Promoted α-MSH-Induced Melanogenesis in B16F10 Melanoma Cells, Which Was Inverse to Resveratrol. Molecules. 2021 Oct 1;26(19):5959. doi: 10.3390/molecules26195959. PMID: 34641503; PMCID: PMC8512250. |
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Cell experiment [1]: |
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Cell lines |
B16F10 cells |
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Preparation method |
Cells were incubation with α-MSH. |
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Reaction Conditions |
1 μM, 72 h |
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Applications |
In α-MSH-stimulated B16F10 cells, autophagosomes engulfing melanosomes following Pt treatment. |
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Animal experiment [2]: |
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Animal models |
TgCRND8 mouse model of AD |
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Preparation method |
Mice were treated with either α-MSH or vehicle via daily intraperitoneal injections for 28 d. |
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Dosage form |
0.5mg/kg;i.p;28days |
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Applications |
α-MSH modulated the excitatory-inhibitory balance in the brain by restoring GABAergic inhibition and improved cognition in TgCRND8 mice. |
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References: [1]. Hseu YC, Vudhya Gowrisankar Y, et,al.The in vitro and in vivo depigmenting activity of pterostilbene through induction of autophagy in melanocytes and inhibition of UVA-irradiated α-MSH in keratinocytes via Nrf2-mediated antioxidant pathways. Redox Biol. 2021 Aug;44:102007. doi: 10.1016/j.redox.2021.102007. Epub 2021 May 19. PMID: 34049220; PMCID: PMC8167190. [2].Biros DJ, Namba K, et,al.Alpha-MSH regulates protein ubiquitination in T cells. Cell Mol Biol (Noisy-le-grand). 2006 May 30;52(2):33-8. PMID: 16914084; PMCID: PMC4698146. |
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| Cas No. | SDF | ||
| 别名 | Ac-Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Val-amide, α-MSH, amide | ||
| 分子式 | C77H109N21O19S1 | 分子量 | 1664.9 |
| 溶解度 | ≥ 166.5mg/mL in DMSO with gentle warming | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.6006 mL | 3.0032 mL | 6.0064 mL |
| 5 mM | 0.1201 mL | 0.6006 mL | 1.2013 mL |
| 10 mM | 0.0601 mL | 0.3003 mL | 0.6006 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet