ABTS diammonium salt (AzBTS-(NH4)2)
(Synonyms: 2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐,AzBTS-(NH4)2) 目录号 : GC33439
A radical cation used to assess antioxidant capacity
Cas No.:30931-67-0
Sample solution is provided at 25 µL, 10mM.
ABTS is a radical cation and a substrate of peroxidases, including horseradish peroxidase (HRP).1 It has commonly been used to assess antioxidant capacity in the Trolox equivalent antioxidant capacity (TEAC) assay.2,3 It has a blue color in the presence of sodium persulfate or metmyoglobin but decolorizes upon incubation with antioxidants, and the antioxidant capacity can be determined spectrophotometrically. ABTS has also been used as an enzyme substrate in ELISAs.4,5 Upon incubation with a peroxidase, it produces a soluble green product that can be quantified by colorimetric detection at 405 nm.1
1.Bhattarai, H.D., Paudel, B., Lee, H.S., et al.Antioxidant activity of Sanionia uncinata, a polar moss species from King George Island, AntarcticaPhytother. Res.22(12)1635-1639(2008) 2.Huang, D., Ou, B., and Prior, R.L.The chemistry behind antioxidant capacity assaysJ. Agric. Food Chem.53(6)1841-1856(2005) 3.Walker, R.B., and Everette, J.D.Comparative reaction rates of various antioxidants with ABTS radical cationJ. Agric. Food Chem.57(4)1156-1161(2009) 4.Reyna-Bello, A., Eleizalde, M.C., and Silva, A.M.Assessment of chromogen suitability in ELISA for the detection of anaplasmosis and trypanosomosisJ. Immunoassay Immunochem.28(1)1-11(2007) 5.Su, Y.C., and Wong, A.C.Detection of staphylococcal enterotoxin H by an enzyme-linked immunosorbent assayJ. Food Prot.59(3)327-330(1996)
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Kinase experiment: |
Enzyme activity is determined photometrically using a temperature controlled multi-mode plate reader or alternatively in a UV/Vis spectrophotometer. Reactions are initiated by addition of the enzyme. Enzyme activity is measured over a period of 10 min at 25°C at the appropriate wavelength for the substrate. One unit (1 U) is defined as the amount of enzyme that converts 1 µmol substrate per minute. Various H2O2 concentrations (0-1250 µM, enzyme concentrations (0.27-54 nM) and substrate concentrations are used to determine the enzyme activity. The activity of rPsaDyP vs ABTS is determined in 100 mM sodium acetate buffer at pH 3.8 and a final H2O2 concentration of 125 µM. Production of the ABTS cation radical is studied at 420 nm (ε420 36,000 L mol-1 cm-1)[2]. |
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References: [1]. Matsuda H, et al. Evaluation of ELISA with ABTS, 2-2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as the substrate of peroxidase and its application to the diagnosis of schistosomiasis. Jpn J Exp Med. 1984 Jun;54(3):131-8. |
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| Cas No. | 30931-67-0 | SDF | |
| 别名 | 2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐,AzBTS-(NH4)2 | ||
| Canonical SMILES | CCN1C(C=CC(S(=O)([O-])=O)=C2)=C2S/C1=N/N=C(SC3=C4C=CC(S(=O)([O-])=O)=C3)\N4CC.[NH4+].[NH4+] | ||
| 分子式 | C18H24N6O6S4 | 分子量 | 548.68 |
| 溶解度 | Water : ≥ 50 mg/mL (91.13 mM) | 储存条件 | Store at 2-8°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8226 mL | 9.1128 mL | 18.2256 mL |
| 5 mM | 0.3645 mL | 1.8226 mL | 3.6451 mL |
| 10 mM | 0.1823 mL | 0.9113 mL | 1.8226 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet