Ac-Calpastatin (184-210)
(Synonyms: Calpastatin Peptide B27-WT) 目录号 : GC40554
A specific inhibitor of calpains I and II
Cas No.:79079-11-1
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
The calpains are a family of calcium-dependent cysteine proteases, with calpain I (µ-calpain) requiring micromolar calcium and calpain II (m-calpain) requiring millimolar calcium. Ac-calpastatin (184-210) is an acetylated synthetic peptide from human calpastatin that strongly inhibits both calpains I and II but not papain (a cysteine protease) or trypsin (a serine protease). This 27 amino acid peptide is encoded by exon 1B of Ac-calpastatin (184-210) and corresponds to a portion of inhibitory domain 1.
| Cas No. | 79079-11-1 | SDF | |
| 别名 | Calpastatin Peptide B27-WT | ||
| Canonical SMILES | NC([C@@H](NC([C@H](CC(C)C)NC([C@H](CC(C)C)NC([C@H](CCC(O)=O)NC([C@H](CCCNC(N)=N)NC([C@@H](NC([C@H](CCCCN)NC([C@@H]1CCCN1C([C@@H]2CCCN2C([C@@]([C@H](CC)C)([H])NC([C@@]([C@@H](C)O)([H])NC([C@H](C(C)C)NC([C@H](CCC(O)=O)NC([C@H](CCCNC(N)=N)NC([C@H](CCCCN | ||
| 分子式 | C142H230N36O44S | 分子量 | 3177.7 |
| 溶解度 | Water: 1 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.3147 mL | 1.5735 mL | 3.1469 mL |
| 5 mM | 0.0629 mL | 0.3147 mL | 0.6294 mL |
| 10 mM | 0.0315 mL | 0.1573 mL | 0.3147 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Cancer screening in the United States, 2019: A review of current American Cancer Society guidelines and current issues in cancer screening
CA Cancer J Clin 2019 May;69(3):184-210.PMID:30875085DOI:10.3322/caac.21557.
Each year, the American Cancer Society publishes a summary of its guidelines for early cancer detection, data and trends in cancer screening rates, and select issues related to cancer screening. In this issue of the journal, the current American Cancer Society cancer screening guidelines are summarized, and the most current data from the National Health Interview Survey are provided on the utilization of cancer screening for men and women and on the adherence of men and women to multiple recommended screening tests.
Protective effect of calpain inhibitors against manganese-induced toxicity in rats
Metab Brain Dis 2022 Apr;37(4):1003-1013.PMID:35089484DOI:10.1007/s11011-022-00916-7.
Development of manganism is a major complication of manganese exposure in which neurological dysfunction is linked to accumulation of metal in the brain. Current therapies do not prevent progression of the disease. Therefore, development of effective therapeutic strategies for treatment of manganism is of utmost importance. Since the hyperactivation of calpain family proteases in CNS during manganism in an animal model is observed, we assumed that inhibition of calpains can suppress the development of Mn-induced neurological disturbances. The goal of this study is to delineate protective effect and the mechanism of neuroprotection of calpain inhibitor in rat model of Mn-induced neurological symptoms. Using the Gait analysis test, we found that chronic intranasal administration of the calpain inhibitor Cast (184-210) (peptide, which is corresponding to the 184-210 amino acid of the endogenous inhibitor of calpains-human calpastatin) to Mn-treated rats contributed to a significant decrease in the severity of gait disorders, although it did not lead to a decrease in the Mn deposition in the striatum and hippocampus. Accordingly to the results of PCR-RT, this effect was accompanied by a partial reduction in the content of neuro-inflammatory markers (IL-1β, TNF-α, NFκB mRNA in the hippocampus and, additionally, IBA-1 mRNA in the striatum), as well as normalization of the content of dopamine and its metabolites in the hippocampus and striatum, which was assessed by HPLC. In striatum cells, the application of Cast (184-210) also led to a significant increase in the production of tyrosine hydroxylase, which was analyzed by immunoblotting method. These findings suggest that calpain inhibitors may be a valid therapeutic agent in manganism.
Structure-function relationship of Vpr: biological implications
Curr HIV Res 2009 Mar;7(2):184-210.PMID:19275588DOI:10.2174/157016209787581490.
Vpr, incorporated into the HIV-1 virion, shows multiple activities including nuclear transport of the preintegration complex to the nucleus, activation of the transcription, cell cycle arrest at the G2/M transition and induction of apoptosis. Vpr controls many host cell functions through a variety of biological activities and by interaction with cellular biochemical pathways. Nuclear import of Vpr may be due to its interaction with nuclear transport factors and components of the nuclear pore complex. Cell cycle arrest has been correlated with the binding to DCAF1, a cullin 4A-associated factor and apoptosis may be facilitated by interaction with mitochondrial proteins, in a caspase-dependent mechanism. The structure of Vpr(1-96) and various fragments have been determined by NMR in diverse solvents. The different functions of Vpr can be classified according to their relationship with the different structural domains of the protein and appear to correlate with the partners interacting with these domains. Thus, virion packaging seems to be mediated by the first alpha-helix (17-33), activation of the transcription, regulation of apoptosis and subcellular transport appear to be dependent on the second alpha-helix (38-50) and cell cycle arrest seems to be induced by the carboxyl terminal alpha-helix (55-77). Mutational analysis performed by several groups have provided a strong basis to understand the structure-function relationship of Vpr. The aim of this review is to run through these mutations using the available information on sequences and discuss their effect on the functions of Vpr from the point of view of its structure.
The validity of self-reported cost events by substance abusers. Limits, liabilities, and future directions
Eval Rev 2001 Apr;25(2):184-210.PMID:11317716DOI:10.1177/0193841X0102500204.
The following review considers data on the validity of self-reports in addict populations, and then it discusses (a) the types of cost-related questions and the assumptions underlying them that are useful to the evaluation of addictions treatment, (b) both internal and external sources of invalidity, (c) the limits on cost-related information that is gathered from administrative databases, (d) methods for assessing measure validity, and (e) the means for improving the validity of self-reports of cost events. With some important exceptions, addicts provide valid data about both medical and criminal cost events. Skilled socioeconomic researchers able to monetarize these events should be able to produce significant cost of illness, cost offset, cost-benefit, and cost-effectiveness research using self-report data.
An initial investigation of dental morphology variation among three southern Naga ethnic groups of Northeast India
Am J Biol Anthropol 2022 Oct;179(2):184-210.PMID:36790681DOI:10.1002/ajpa.24605.
Objectives: This study examines dental morphology trait prevalence among three southern Naga groups and compares them to 10 ethnic groups from other regions of South Asia to accomplish two objectives: assess the biological relationship of these Tibeto-Burman-speakers to speakers of non-Tibeto-Burman languages in other South Asian regions, and determine which traits distinguish northeast Indians from other South Asians. Methods: Dental morphology traits were scored with the Arizona State University Dental Anthropology System. Tooth-trait combinations were evaluated for significant inter-trait correlation and intra-trait correspondence within dental fields. Comparisons were based on simple trait prevalence and with Smith's MMD. Affinities based on the former were accomplished with correspondence analysis and principal components analysis. Affinities based on the latter were undertaken with neighbor-joining cluster analysis and multidimensional scaling. Results: After elimination due to inter-trait correlations and uniform prevalence, biodistances based on the remaining 17 tooth-trait combinations identify significant differences between northeast Indians and other South Asian ethnic groups due to high frequencies of shoveling on the maxillary incisors and Cusp 6 on the mandibular molars coupled with low frequencies of Carabelli's trait and Cusp 5 on UM1 and UM2, respectively. Conclusions: Patterns of biodistances obtained from dental morphology are consilient with those obtained from DNA indicating statistically significant differences between northeast Indians from members of ethnic groups of other regions of South Asia. Researchers should explore the sex-specific patterns. Biodistances should not be limited to "key" teeth within dental fields, for in almost every case traits present on mesial and distal teeth yield non-redundant information.