Acequinocyl
(Synonyms: 灭螨醌) 目录号 : GC46779
A naphthoquinone acaricide
Cas No.:57960-19-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Acequinocyl is a naphthoquinone acaricide that inhibits complex III of the mitochondrial electron transport chain in mites.1 Weekly application of acequinocyl (0.03 kg AI/100 L) to strawberry plants reduces seasonal averages of the number of T. urticae mites and mite eggs on plant leaflets.2 It is not toxic to rats or mice (LD50s = >5,000 mg/kg).1 Formulations containing acequinocyl have been used to control mite populations in agriculture.
1.Registration & Regulatory Affairs Group, Development Department., Agro-Kanesho Co., Ltd.Summary of toxicological studies on acequinocylJ. Pestic. Sci.26(4)433-438(2001) 2.Schuster, D.J., Price, J.F., Howard, C.M., et al.Twospotted spider mites: Control on strawberry with organotin, naphthoquinone, and cyclopropane acaricidesJ. Econ. Entomol.72(3)360-361(1979)
| Cas No. | 57960-19-7 | SDF | |
| 别名 | 灭螨醌 | ||
| Canonical SMILES | O=C1C(CCCCCCCCCCCC)=C(OC(C)=O)C(C2=CC=CC=C21)=O | ||
| 分子式 | C24H32O4 | 分子量 | 384.5 |
| 溶解度 | Chloroform: Slightly Soluble,Methanol: Heated | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.6008 mL | 13.0039 mL | 26.0078 mL |
| 5 mM | 0.5202 mL | 2.6008 mL | 5.2016 mL |
| 10 mM | 0.2601 mL | 1.3004 mL | 2.6008 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Acequinocyl Resistance Associated With I256V and N321S Mutations in the Two-Spotted Spider Mite (Acari: Tetranychidae)
J Econ Entomol 2019 Mar 21;112(2):835-841.PMID:30615159DOI:10.1093/jee/toy404.
The two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), is one of the most important pest species, because it devastates many horticultural and ornamental crops and fruit trees. In the present study, we explored a field strain that was collected in January 2001 and then selected for 16 years for Acequinocyl resistance. The resistance ratios calculated for the LC50 value in the laboratory-selected acequinocyl-resistant (LSAR16) strain was 4,237-fold higher than that of the susceptible strain. Pretreatment with the synergists piperonyl butoxide and S,S,S-tributyl-phosphorotrithioate significantly increased the toxicity of Acequinocyl to the LSAR16 strain. Crossing experiments revealed that the resistance in the LSAR16 strain was maternally inherited, dominant, and monogenic. Furthermore, among individuals in the LSAR16 strain, 85.5-98.5% had the I256V mutation and 98-99% had the N321S mutation in mitochondrial cytochrome b. These results suggest that these two new point mutations contribute to Acequinocyl resistance in T. urticae.
Modification of the existing maximum residue level for Acequinocyl in sweet peppers/bell peppers
EFSA J 2022 Mar 4;20(3):e07175.PMID:35281641DOI:10.2903/j.efsa.2022.7175.
In accordance with Article 6 of Regulation (EC) No 396/2005, the applicant Agro-Kanesho submitted a request to the competent national authority in Germany to modify the existing maximum residue level (MRL) for the active substance Acequinocyl in sweet peppers/bell peppers. The data submitted in support of the request were found to be sufficient to derive MRL proposal for peppers. Adequate analytical methods for enforcement are available to control the residues of Acequinocyl in peppers at the validated limit of quantification (LOQ) of 0.01 mg/kg. Based on the risk assessment results, EFSA concluded that the short-term and long-term intake of residues resulting from the use of Acequinocyl according to the reported agricultural practice is unlikely to present a risk to consumer health.
Modification of the existing maximum residue levels for Acequinocyl in citrus fruits
EFSA J 2019 Aug 6;17(8):e05746.PMID:32626402DOI:10.2903/j.efsa.2019.5746.
In accordance with Article 6 of Regulation (EC) No 396/2005, the applicant Agro-Kanesho submitted a request to the competent national authority in Germany to modify the existing maximum residue level (MRLs) for the active substance Acequinocyl in citrus fruits. The data submitted in support of the request were found to be sufficient to derive MRL proposals for grapefruits, oranges, lemons, limes and mandarins. Adequate analytical methods for enforcement are available to control the residues of Acequinocyl in the commodities under consideration at the validated limit of quantification (LOQ) of 0.01 mg/kg. Based on the risk assessment results, EFSA concluded that the short-term and long-term intake of residues resulting from the use of Acequinocyl according to the reported agricultural practice is unlikely to present a risk to consumer health.
Identification and characterization of new mutations in mitochondrial cytochrome b that confer resistance to bifenazate and Acequinocyl in the spider mite Tetranychus urticae
Pest Manag Sci 2020 Mar;76(3):1154-1163.PMID:31599486DOI:10.1002/ps.5628.
Background: In spider mites, mutations in the mitochondrial cytochrome b Qo pocket have been reported to confer resistance to the Qo inhibitors bifenazate and Acequinocyl. In this study, we surveyed populations of the two-spotted spider mite Tetranychus urticae for mutations in cytochrome b, linked newly discovered mutations with resistance and assessed potential pleiotropic fitness costs. Results: We identified two novel mutations in the Qo site: G132A (equivalent to G143A in fungi resistant to strobilurins) and G126S + A133T (previously reported to cause bifenazate and Acequinocyl resistance in Panonychus citri). Two T. urticae strains carrying G132A were highly resistant to bifenazate but not Acequinocyl, whereas a strain with G126S + A133T displayed high levels of Acequinocyl resistance, but only moderate levels of bifenazate resistance. Bifenazate and Acequinocyl resistance were inherited maternally, providing strong evidence for the involvement of these mutations in the resistance phenotype. Near isogenic lines carrying G132A revealed several fitness penalties in T. urticae; a lower net reproductive rate (R0 ), intrinsic rate of increase (rm) and finite rate of increase (LM); a higher doubling time (DT); and a more male-biased sex ratio. Conclusions: Several lines of evidence were provided to support the causal role of newly discovered cytochrome b mutations in bifenazate and Acequinocyl resistance. Because of the fitness costs associated with the G132A mutation, resistant T. urticae populations might be less competitive in a bifenazate-free environment, offering opportunities for resistance management. © 2019 Society of Chemical Industry.
Determination of Acequinocyl and hydroxyacequinocyl on fruits and vegetables by HPLC-DAD
J Agric Food Chem 2004 Nov 3;52(22):6700-2.PMID:15506803DOI:10.1021/jf0487304.
A method for determining residues of the new reduced-risk pesticide Acequinocyl and its deacetylated derivative hydroxyacequinocyl on fruits and vegetables (grapes, lemons, pears, and tomatoes) by HPLC is described. The pesticides were extracted from the fruits and vegetables with hexane and ethyl acetate solution (1:1, v/v), determined by HPLC-DAD at 250 nm and confirmed by LC/MS. No cleanup was necessary. This method is characterized by recoveries (0.01-4 mg/kg) > 77%, while the coefficient of variation was determined to be less than 11%. The limit of quantitation for both Acequinocyl and hydroxyacequinocyl was 0.01 mg/kg for all matrixes.