Acetoacetyl Coenzyme A (sodium salt hydrate)
(Synonyms: Acetoacetyl-CoA) 目录号 : GC42695A precursor to HMG-CoA
Sample solution is provided at 25 µL, 10mM.
Acetoacetyl-coenzyme A (acetoacetyl-CoA) is a precursor to HMG-CoA in the isoprenoid pathway.[1],[2] It is reversibly converted to acetyl-CoA by acetoacetyl-CoA thiolase in the mitochondria. Acetoacetyl-CoA thiolase (T2) deficiency results in a build-up of ketone bodies leading to intermittent ketoacidosis.[3],[4] Acetoacetyl-CoA is also an intermediate in the microbial biosynthesis of polyhydroxybutyrate.[5]
References:
[1]. Lynen, F., and Ochoa, S. Enzymes of fatty acid metabolism. Biochem. Biophys. Acta. 12(1-2), 299-314 (1953).
[2]. Miziorko, H.M. Enzymes of the mevalonate pathway of isoprenoid biosynthesis. Arch. Biochem. Biophys. 505(2), 131-143 (2011).
[3]. Daum, R.S., Scriver, C.R., Mamer, O.A., et al. An inherited disorder of isoleucine catabolism causing accumulation of alpha-methylacetoacetate and alpha-methyl-beta -hydroxybutyrate, and intermittent metabolic acidosis. Pediatr. Res. 7(3), 149-160 (1973).
[4]. Fukao, T., Scriver, C.R., and Kondo, N. The clinical phenotype and outcome of mitochondrial acetoacetyl-CoA thiolase deficiency (β-ketothiolase or T2 deficiency) in 26 enzymatically proved and mutation-defined patients. Mol. Genet. Metab. 72(2), 109-114 (2001).
[5]. Jacquel, N., Lo, C.-W., Wei, Y.-H., et al. Isolation and purification of bacterial poly(3-hydroxyalkanoates). Biochem. Eng. J. 39(1), 15-27 (2008).
Cas No. | SDF | ||
别名 | Acetoacetyl-CoA | ||
化学名 | S-(3-oxobutanoate) coenzyme A, trisodium salt, tetrahydrate | ||
Canonical SMILES | O[C@H]1[C@H](N2C=NC3=C2N=CN=C3N)O[C@H](COP(OP(OCC(C)(C)[C@@H](O)C(NCCC(NCCSC(CC(C)=O)=O)=O)=O)([O-])=O)([O-])=O)[C@H]1OP(O)([O-])=O.[Na+].[Na+].[Na+].O.O.O.O | ||
分子式 | C25H37N7O18P3S•3Na [4H2O] | 分子量 | 989.6 |
溶解度 | 10mg/mL in PBS, pH 7.2 | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 1.0105 mL | 5.0525 mL | 10.1051 mL |
5 mM | 0.2021 mL | 1.0105 mL | 2.021 mL |
10 mM | 0.1011 mL | 0.5053 mL | 1.0105 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet