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ACSF Sale

目录号 : GC11850

replacement of CSF

ACSF Chemical Structure

规格 价格 库存 购买数量
25ml
¥1,985.00
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Sample solution is provided at 25 µL, 10mM.

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产品描述

ACSF is often used as replacement of CSF for perfusion of brain slices to preserve interneurons [1].
ACSF (artificial cerebrospinal fluid) is invented to reduce the incidence of cerebral edema and further suppress brain cell disorders. And ACSF is often used as an irrigation fluid or perfusion fluid in the field of neurosurgery, such as intracranial surgery, or when used as a replenishing fluid for lost cerebrospinal fluid. ACSF is also used to maintain pH balance and tissue oxygen delivery for research [2, 3].
ACSF is often used to maintain oxygen supply in the study of basic characteristics of neuronal function where acute in vitro brain slice models are required. When tested with neocortical mouse slices, carbogenated ACSF treatment could keep pH balance and tissue oxygen delivery; otherwise, seizure-like events were occurred in the slices with no-magnesium ACSF treatment [1]. In the study of targeted drugs for protect neuron, ACSF is often to treat the mice and worked as a control to calculate the drug efficiency [3]
It is also reported in the rat brain slice model, treated with ACSF, a replacement of human CSF, could powerfully boost spontaneous firing of CA1, CA3 and layer 5 pyramidal neurons [4].
References:
[1].    Voss, L.J., S.A. George, and J.W. Sleigh, Testing neocortical slice viability in non-perfused no-magnesium artificial cerebrospinal fluid solutions. J Neurosci Methods, 2012. 204(2): p. 273-5.
[2].    Liu, D. and F. Bao, Hydrogen peroxide administered into the rat spinal cord at the level elevated by contusion spinal cord injury oxidizes proteins, DNA and membrane phospholipids, and induces cell death: Attenuation by a metalloporphyrin. Neuroscience, 2015. 285: p. 81-96.
[3].    Gruber, R.C., et al., Targeted GAS6 delivery to the CNS protects axons from damage during experimental autoimmune encephalomyelitis. J Neurosci, 2014. 34(49): p. 16320-35.
[4].    Bjorefeldt, A., et al., Human cerebrospinal fluid increases the excitability of pyramidal neurons in the in vitro brain slice. J Physiol, 2015. 593(1): p. 231-43.

Chemical Properties

Cas No. SDF
分子式 C20H20N2O2 分子量 320.38
溶解度 Soluble to 5 mM in ethanol with gentle warming 储存条件 Store at RT
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

制备储备液
1 mg 5 mg 10 mg
1 mM 3.1213 mL 15.6065 mL 31.2129 mL
5 mM 0.6243 mL 3.1213 mL 6.2426 mL
10 mM 0.3121 mL 1.5606 mL 3.1213 mL
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动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % saline
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