AD 0261
目录号 : GC30451
AD0261是一种自由基清除剂，能够有效抑制脂质过氧化物和超氧化物阴离子的产生。
Cas No.:145600-69-7
Sample solution is provided at 25 µL, 10mM.
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AD 0261 is a radical scavenger which displays strong inhibitory action on the generation of lipid peroxides and superoxide anions.
AD 0261 displays strong inhibitory action on the generation of lipid peroxides (IC50=0.022 μM) and superoxide anions (IC50= 0.9-4.0 μM). AD 0261 inhibits cytokine release and/or production from PHA-P-stimulated lymphocytes in a dose-dependent manner. AD 0261 inhibits all parameters which are indicative of lymphocyte activation with 50% inhibition being achieved in a range of 1-10 μM. AD 0261 suppresses ornithine decarboxylase activity, the IC50 value being around 1.0 μM[1].
[1]. M. Sugiura, et al. Effect of the novel radical scavenger AD0261 on lymphocyte activation. Information Research. Agents and Actions. August 1994, Volume 41, Supplement 2, pp C216-C218.
Cell experiment: | For measurement of [3H]TdR incorporation, cells are incubated for 16 h and additional (6 h) incubation is carried out in the presence of [3H]TdR (0.5 μCi/well). [3H]TdR incorporation is evaluated in a liquid scintillation counter. IL-2 release from lymphocytes is estimated after 20 h incubation by enzyme immunoassay using the commercially available kits. IL-2 receptor expression is quantitated by flow-cytometry using an FITC-labelled monoclonal antibody which recognizes achain of IL-2 receptor complex. Cell viability is determined by either trypan blue exclusion test or propidium iodide staining. |
References: [1]. M. Sugiura, et al. Effect of the novel radical scavenger AD0261 on lymphocyte activation. Information Research. Agents and Actions. August 1994, Volume 41, Supplement 2, pp C216-C218. | |
| Cas No. | 145600-69-7 | SDF | |
| Canonical SMILES | OC1=CC=C(C)C=C1NCCCN2CCN(C(C3=CC=C(F)C=C3)C4=CC=C(F)C=C4)CC2 | ||
| 分子式 | C27H31F2N3O | 分子量 | 451.55 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.2146 mL | 11.073 mL | 22.1459 mL |
| 5 mM | 0.4429 mL | 2.2146 mL | 4.4292 mL |
| 10 mM | 0.2215 mL | 1.1073 mL | 2.2146 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet