Adapalene-d3
(Synonyms: 阿达帕林 d3) 目录号 : GC46798An internal standard for the quantification of adapalene
Cas No.:1276433-89-6
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Adapalene-d3 is intended for use as an internal standard for the quantification of adapalene by GC- or LC-MS. Adapalene is a synthetic retinoid and an agonist of retinoic acid receptors (RARs; Kds = 1,100, 34, and 130 nM for RARα, RARβ, and RARγ, respectively).1,2,3,4,5 It inhibits growth and differentiation of sebocytes in a concentration-dependent manner in primary rat preputial cell culture. Adapalene (10 μM) completely inhibits the activity of soybean 15-lipoxygenase (15-LOX) in an enzyme assay and inhibits the 5- and 15-LOX pathways in human blood polymorphonuclear leukocytes (PMNs).2 It reduces the protein levels of toll-like receptor 2 (TLR2) and IL-10 in skin explants isolated from patients with acne and healthy controls in a concentration-dependent manner but increases the expression of the antigen-presenting protein CD1d in acne skin explants while decreasing it in control explants.3 It inhibits inflammation in rodent models of ear edema induced by arachidonic acid and carrageenan-induced paw edema.2 Adapalene (100 μM) also induces apoptosis and inhibits proliferation of CC-531, HT-29, and LoVo colon cancer cells and reduces tumor growth in a DLD-1 colon cancer nude mouse xenograft model in a dose-dependent manner.5,4 Formulations containing adapalene have been used in the treatment of acne vulgaris.
1.Kim, M.J., Ciletti, N., Michel, S., et al.The role of specific retinoid receptors in sebocyte growth and differentiation in cultureJournal of Investigative Dermatology114349-353(2000) 2.Hensby, C., Cavey, D., Bouclier, M., et al.The in vivo and in vitro anti-inflammatory activity of CD271: A new retinoid-like modulator of cell differentiationAgents Actions29(1-2)56-58(1990) 3.Tenaud, I., Khammari, A., and Dreno, B.In vitro modulation of TLR-2, CD1d and IL-10 by adapalene on normal human skin and acne inflammatory lesionsExp. Dermatol.16(6)500-506(2007) 4.Ocker, M., Herold, C., Ganslmayer, M., et al.The synthetic retinoid adapalene inhibits proliferation and induces apoptosis in colorectal cancer cells in vitroInt. J. Cancer107(3)453-459(2003) 5.Shi, X.-N., Li, H., Yao, H., et al.Adapalene inhibits the activity of cyclin-dependent kinase 2 in colorectal carcinomaMol. Med. Rep.12(5)6501-6508(2015)
Cas No. | 1276433-89-6 | SDF | |
别名 | 阿达帕林 d3 | ||
Canonical SMILES | [2H]C([2H])([2H])OC1=C(C2(C[C@H](C3)C4)C[C@H]4C[C@H]3C2)C=C(C=C1)C(C=C5)=CC6=C5C=C(C(O)=O)C=C6 | ||
分子式 | C28H25D3O3 | 分子量 | 415.5 |
溶解度 | DMF: 5 mg/ml,DMSO: 5 mg/ml | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 2.4067 mL | 12.0337 mL | 24.0674 mL |
5 mM | 0.4813 mL | 2.4067 mL | 4.8135 mL |
10 mM | 0.2407 mL | 1.2034 mL | 2.4067 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。