Adenosine-5'-diphosphate
(Synonyms: 二磷酸腺苷,Adenosine Pyrophosphate,ADP,5′-ADP) 目录号 : GC11969
ATP precursor and purinergic receptor ligand
Cas No.:58-64-0
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
IC50: 67 nM for P2X2/3
Adenosine-5'-diphosphate is an agonist of purinergic receptors.
Purinergic receptors, also known as purinoceptors, are a family of plasma membrane molecules that are found in almost all mammalian tissues. Within the field of purinergic signalling, these receptors have been implicated in learning and memory, locomotor and feeding behavior, and sleep. More specifically, purinergic receptors are involved in several cellular functions, such as proliferation and migration of neural stem cells, vascular reactivity, apoptosis and cytokine secretion.
In vitro: Adenosine 5'-diphosphate (ADP) is an adenine nucleotide having two phosphate groups esterified to the sugar moiety at the 5’ position. ADP is formed through dephosphorylation of adenosine 5’-triphosphate (ATP) by ATPases and can be converted back to ATP by ATP synthases. ADP can also be metabolized to adenosine 5’-monophosphate (AMP) and 2’-deoxyadenosine 5’-diphosphate (dADP). ADP can modulate several receptors, such as activating certain purinergic receptors and inhibiting others, inhibiting rat ecto-5’nucleotidase (Ki = 0.91 nM), as well as regulating the phosphorylation status of AMP-activated protein kinase [1, 2].
In vivo: Up to now, there is no animal in vivo data reported.
Clinical trial: So far, no clinical study has been conducted.
IC50: P2X2/3为67 nM
腺苷酸二钠是嘌呤能受体的一种激动剂。
嘌呤能受体，也称为嘌呤受体，是一类存在于几乎所有哺乳动物组织中的质膜分子。在嘌呤能信号传导领域，这些受体被认为与学习和记忆、运动和进食行为以及睡眠等方面有关。更具体地说，嘌呤能受体参与了多种细胞功能，例如神经干细胞的增殖和迁移、血管反应性、细胞凋亡和细胞因子分泌。
体外实验：腺苷酸二钠（ADP）是一种腺嘌呤核苷酸，其两个磷酸基被酯化到5'位的糖基上。ADP通过ATP酶的去磷酸化形成，可以通过ATP合成酶转化回ATP。ADP还可以代谢成腺苷酸单钠（AMP）和2'-去氧腺苷酸二钠（dADP）。ADP可以调节多种受体，例如激活某些嘌呤能受体并抑制其他受体，抑制大鼠外泌5'nucleotidase（Ki = 0.91 nM），以及调节AMP-激活蛋白激酶的磷酸化状态 [1, 2]。
体内实验：目前还没有报告动物体内数据。
临床研究：目前还没有进行任何临床研究。
References:
1. Azran, S.,Frster, D.,Danino, O., et al. Highly efficient biocompatible neuroprotectants with dual activity as antioxidants and P2Y receptor agonists. J. Med. Chem. 56(12), 4938-4952 (2013).
2. Jarvis, M.F.,Bianchi, B.,Uchic, J.T., et al. [3H]A-317491, a novel high-affinity non-nucleotide antagonist that specifically labels human P2X2/3 and P2X3 receptors. Journal of Pharmacology and Experimental Therapeutics 310(1), 407-416 (2004).
| Cas No. | 58-64-0 | SDF | |
| 别名 | 二磷酸腺苷,Adenosine Pyrophosphate,ADP,5′-ADP | ||
| 化学名 | adenosine 5'-(trihydrogen diphosphate) | ||
| Canonical SMILES | O[C@H]1[C@H](N2C=NC3=C2N=CN=C3N)O[C@H](COP(OP(O)(O)=O)(O)=O)[C@H]1O | ||
| 分子式 | C10H15N5O10P2 | 分子量 | 427.2 |
| 溶解度 | ≥ 21.35mg/mL in DMSO with gentle warming | 储存条件 | Store at -20°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.3408 mL | 11.7041 mL | 23.4082 mL |
| 5 mM | 0.4682 mL | 2.3408 mL | 4.6816 mL |
| 10 mM | 0.2341 mL | 1.1704 mL | 2.3408 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。