Adrenalone HCl
(Synonyms: 肾上腺酮盐酸盐) 目录号 : GC13814
Decoyinine (Angustmycin A), a specific inhibitor of bacterial GMPS, has been isolated from Streptomyces .
Cas No.:62-13-5
Sample solution is provided at 25 µL, 10mM.
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Decoyinine (Angustmycin A), a specific inhibitor of bacterial GMPS, has been isolated from Streptomyces hygroscopius as a potential antibiotic with sporulation-inducing activity in Bacillus subtilis[1-3]. Angustmycin A was also recently found to be a promising cytokinin that induces adventitious root or bud differentiation[5].
With 2 mM of Angustmycin A treatment of SK-Mel-28 and SK-Mel103 cells reduced their invasion by ~30% compared with vehicle-treated cells[4]. Decoyinine (Angustmycin A) (>1mM) enabled the S. griseus IFO 13189 cells to develop aerial mycelia in the suppressed cultures at concentrations which only partially inhibited growth[6].
Decoyinine (Angustmycin A)(120 mg/kg; i.p., 10days) treatment resulted in a 36% reduction of xenografts volume while MMF caused only 19% volume reduction in SK-Mel-103 mcie. In SK-Mel-28 mcie, the xenograft volume reduction caused by angustmycin A(120 mg/kg; i.p., 27days) and MMF were of 62% and 30%, respectively[4].
References:
[1]. Vasantha N, Freese E. Enzyme changes during Bacillus subtilis sporulation caused by deprivation of guanine nucleotides. J Bacteriol. 1980 Dec;144(3):1119-25. doi: 10.1128/jb.144.3.1119-1125.1980. PMID: 6777366; PMCID: PMC294778.
[2]. Ikehara K, Okamoto M, et,al. Induction of Bacillus subtilis sporulation by decoyinine and the concomitant disappearance of ppGpp in vegetative cells. J Biochem 1982; 91: 1089-1092.
[3]. Mitani T, Heinze JE, et,al. Induction of sporulation in Bacillus subtilis by decoyinine or hadacidin. Biochem Biophys Res Commun 1977; 77: 1118-1125.
[4]. Bianchi-Smiraglia A, Wawrzyniak JA, et,al.Pharmacological targeting of guanosine monophosphate synthase suppresses melanoma cell invasion and tumorigenicity. Cell Death Differ. 2015 Nov;22(11):1858-64. doi: 10.1038/cdd.2015.47. Epub 2015 Apr 24. PMID: 25909885; PMCID: PMC4648332.
[5]. Hong-Yuan, X., Hong-Zhang, X., et,al. in Biotechnology and Sustainable Agriculture 2006 and Beyond (eds. Xu, Z., Li, J., Xue, Y. & Yang, W.) 97-101 (Springer Netherlands, 2010).
[6]. Ochi K. Metabolic initiation of differentiation and secondary metabolism by Streptomyces griseus: significance of the stringent response (ppGpp) and GTP content in relation to A factor. J Bacteriol. 1987 Aug;169(8):3608-16. doi: 10.1128/jb.169.8.3608-3616.1987. PMID: 3112126; PMCID: PMC212439.
| Cell experiment [1]: | |
|
Cell lines |
SK-Mel-28 and SK-Mel103 cells |
|
Preparation Method |
Cells were incubated with 2 mM Decoyinine (Angustmycin A) for 24h before invasion assay. Where indicated, cells were supplemented with 100 µM guanosine. Control cells were treated with equal volumes of DMSO. Drug treatments were maintained throughout the experimental procedure. |
|
Reaction Conditions |
2 mM;24h |
|
Applications |
Treatment of SK-Mel-28 and SK-Mel103 cells with 2 mM of Decoyinine(Angustmycin A) reduced their invasion by ~30% compared with vehicle-treated cells. |
| Animal experiment [2]: | |
|
Animal models |
4-6-week-old female SCID mice |
|
Preparation Method |
SK-Mel-103 and SK-Mel-28 cells were inoculated into both flanks of SCID mice. When tumors reached 100 mm3 in size, mice were randomly assigned to different treatment groups and treated with daily i.p. injection of Decoyinine (Angustmycin A) (120 mg/kg in 10% DMSO in PBS), Mycophenolate Mofetil, or the correspondent vehicle control. |
|
Dosage form |
120 mg/kg; i.p., 10-27days |
|
Applications |
In SK-Mel-103 cells mcie, Decoyinine (Angustmycin A) treatment resulted in a 36% reduction of xenografts volume while MMF caused only 19% volume reduction. In SK-Mel-28 cells mcie, the xenograft volume reduction caused by Decoyinine (Angustmycin A) and MMF were of 62% and 30%, respectively. |
|
References: [1]. Bianchi-Smiraglia A, Wawrzyniak JA, et,al.Pharmacological targeting of guanosine monophosphate synthase suppresses melanoma cell invasion and tumorigenicity. Cell Death Differ. 2015 Nov;22(11):1858-64. doi: 10.1038/cdd.2015.47. Epub 2015 Apr 24. PMID: 25909885; PMCID: PMC4648332. | |
| Cas No. | 62-13-5 | SDF | |
| 别名 | 肾上腺酮盐酸盐 | ||
| 化学名 | 1-(3,4-dihydroxyphenyl)-2-(methylamino)ethanone;hydrochloride | ||
| Canonical SMILES | CNCC(=O)C1=CC(=C(C=C1)O)O.Cl | ||
| 分子式 | C9H11NO3.HCl | 分子量 | 217.65 |
| 溶解度 | ≥ 10.88mg/mL in DMSO | 储存条件 | Store at -20°C,unstable in solution, ready to use. |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 4.5945 mL | 22.9727 mL | 45.9453 mL |
| 5 mM | 0.9189 mL | 4.5945 mL | 9.1891 mL |
| 10 mM | 0.4595 mL | 2.2973 mL | 4.5945 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet