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Adrenomedullin (AM) (22-52), human (22-52-Adrenomedullin (human)) Sale

(Synonyms: 肾上腺髓质素(22-52)(人体),22-52-Adrenomedullin (human)) 目录号 : GC32615

Adrenomedullin (AM) (22-52), human是一种NH2末端截断形式的Adrenomedullin (AM),不具有六元环结构,也不保有降血压和血管舒张活性。

Adrenomedullin (AM) (22-52), human (22-52-Adrenomedullin (human)) Chemical Structure

Cas No.:159899-65-7

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500μg
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1mg
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5mg
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Sample solution is provided at 25 µL, 10mM.

Description

Adrenomedullin (AM) (22-52), human, is a NH2-terminal truncated forms of Adrenomedullin, which don’t possess the six-membered ring structure or retain the hypotensive and vasodilator activity[1, 2]. Adrenomedullin (AM) (22-52), human can be used as an antagonist of Adrenomedullin receptors[3]. Adrenomedullin (AM) (22-52), human antagonizes vasodilator responses to calcitonin gene-related peptide[4].

Adrenomedullin (AM) (22-52), human (10-7M; 60min) inhibited AM (10-7M; 30min) induced cAMP production in BACs[5]. Adrenomedullin (AM) (22-52), human (10-7M; 3 or 24h) abrogated the effect that Adrenomedullin (10-7M; 3 or 24h) evoked a small rise in adrenocorticotropic hormone (ACTH (10-8M; 3 or 24h)) stimulated corticosterone production in rat adrenocortical cells[6].

Adrenomedullin (AM) (22-52), human (250μg/rat/day; i.p. ; 2 weeks) reduced urinary protein excretion in the diabetic rats by some 40%[7]. Adrenomedullin (AM) (22-52), human (50μg/tumor; intratumoral injection; once a day for 10 days) significantly reduced the in vivo growth of the pancreatic cancer cell line, which were inoculated subcutaneously into the right flanks of severe combined immune deficiency (SCID) mice[8].

References:
[1] EGUCHI S, HIRATA Y, IWASAKI H, et al. Structure-activity relationship of adrenomedullin, a novel vasodilatory peptide, in cultured rat vascular smooth muscle cells [J]. Endocrinology, 1994, 135(6): 2454-8.
[2] NOSSAMAN B D, FENG C J, KAYE A D, et al. Pulmonary vasodilator responses to adrenomedullin are reduced by NOS inhibitors in rats but not in cats [J]. Am J Physiol, 1996, 270(5 Pt 1): L782-9.
[3] AKASHI E, NAGATA S, YAMASAKI M, et al. Activation of Calcitonin Gene-Related Peptide and Adrenomedullin Receptors by PEGylated Adrenomedullin [J]. Biol Pharm Bull, 2020, 43(11): 1799-803.
[4] CHAMPION H C, SANTIAGO J A, MURPHY W A, et al. Adrenomedullin-(22-52) antagonizes vasodilator responses to CGRP but not adrenomedullin in the cat [J]. Am J Physiol, 1997, 272(1 Pt 2): R234-42.
[5] VELARD F, CHATRON-COLLIET A, COME D, et al. Adrenomedullin and truncated peptide adrenomedullin(22-52) affect chondrocyte response to apoptotis in vitro: downregulation of FAS protects chondrocyte from cell death [J]. Sci Rep, 2020, 10(1): 16740.
[6] ZIOLKOWSKA A, BUDZYNSKA K, TREJTER M, et al. Effects of adrenomedullin and its fragment 22-52 on basal and ACTH-stimulated secretion of cultured rat adrenocortical cells [J]. Int J Mol Med, 2003, 11(5): 613-5.
[7] EL ETER E A, AL-MASRI A A. Adrenomedullin mediates early phase angiogenesis induced diabetic nephropathy in STZ diabetic rats [J]. Eur Rev Med Pharmacol Sci, 2014, 18(22): 3534-43.
[8] ISHIKAWA T, CHEN J, WANG J, et al. Adrenomedullin antagonist suppresses in vivo growth of human pancreatic cancer cells in SCID mice by suppressing angiogenesis [J]. Oncogene, 2003, 22(8): 1238-42.

Adrenomedullin (AM) (22-52), human是一种NH2末端截断形式的Adrenomedullin (AM),不具有六元环结构,也不保有降血压和血管舒张活性[1,2]。Adrenomedullin (AM) (22-52), human可作为Adrenomedullin (AM) 受体的拮抗剂[3]。Adrenomedullin (AM) (22-52), human拮抗降钙素基因相关肽的血管舒张反应[4]

Adrenomedullin (AM) (22-52), human(10-7M; 60min)可在关节软骨细胞中抑制Adrenomedullin (AM)(10-7M; 30min)诱导产生的cAMP[5]。Adrenomedullin (AM) (22-52), human (10-7M; 3 or 24h)消除大鼠肾上腺细胞内Adrenomedullin (AM)(10-7M; 3 or 24h)诱导ACTH(Adrenocorticotropic Hormone,促肾上腺皮质激素;10-8M; 3 or 24h)小幅升高刺激的皮质酮生成[6]

使用Adrenomedullin (AM) (22-52), human(1μg/kg/day; i.p. ; 3 times a week for 8 weeks)处理糖尿病模型大鼠,Adrenomedullin (AM) (22-52), human下调糖尿病大鼠约40%的尿蛋白排泄量[7]。使用Adrenomedullin (AM) (22-52), human(25μg/kg; i.p. ; 4 day)处理右侧皮下接种的胰腺癌细胞系的SCID(severe combined immune deficiency,严重联合免疫缺陷)小鼠,Adrenomedullin (AM) (22-52), human显著抑制了肿瘤在SCID小鼠的体内生长[8]

实验参考方法

Cell experiment [1]:

Cell lines

Bovine articular chondrocytes (BACs)

Preparation Method

BACs were isolated from the carpal-metacarpal joint of daily slaughtered cows at the SOVIAM slaughter house. Briefly, small pieces of cartilage were harvested from joints, rinsed 3 times before being minced. Small pieces (< 2mm) were rinsed again before enzymatic digestion overnight in bacterial collagenase II at 37℃. Cells were passed through a nylon cell strainer (100μm porosity) to avoid collection of non-digested cartilage before being rinsed, counted and seeded at high density in 15ml DMEM supplemented with 10% FCS, 2mM L-glutamine, 1% antibiotics and fungizone. Cells were cultured in a dedicated incubator at 37℃ in humidified atmosphere containing 5% CO2 and 3% O2 (hypoxic condition) or atmospheric O2 content (normoxic basal condition). Medium was changed twice a week for 2 weeks to obtain confluent cell culture. Cells were then starved overnight, detached by using trypsin/EDTA, rinsed twice, then counted and plated in starvation medium before treatment. Cells were cultured directly on plastic except for TUNEL and immunostaining experiments, for which they were cultured on glass coverslips. For TUNEL and caspase activity measurements, cells were treated for 24h with 20ng/mL Fas-L. In all cases, cells were pretreated for 30min with Adrenomedullin (AM) (22-52) at 10–7M.

Reaction Conditions

10-7M; 30min

Applications

Adrenomedullin (AM) (22-52), human showed significantly reduced cell death in articular chondrocytes.
Animal experiment [2]:

Animal models

Severe combined immune deficiency mice

Preparation Method

PCI-43 cells (5×106) were inoculated subcutaneously into the right flanks of SCID mice. Tumor formation was observed every 3 days up to 3 weeks after inoculation.Tumors were treated daily with intratumoral injection of AM(1-25) or Adrenomedullin (AM) (22-52) from day 8 to day 17 after the inoculation at 50mg/tumor.

Dosage form

50μg/tumor; intratumoral injection; once a day for 10 days

Applications

Adrenomedullin (AM) (22-52), human significantly reduced the in vivo growth of the pancreatic cancer cell line.

References:
[1] VELARD F, CHATRON-COLLIET A, COME D, et al. Adrenomedullin and truncated peptide adrenomedullin(22-52) affect chondrocyte response to apoptotis in vitro: downregulation of FAS protects chondrocyte from cell death [J]. Sci Rep, 2020, 10(1): 16740.
[2] ISHIKAWA T, CHEN J, WANG J, et al. Adrenomedullin antagonist suppresses in vivo growth of human pancreatic cancer cells in SCID mice by suppressing angiogenesis [J]. Oncogene, 2003, 22(8): 1238-42.

化学性质

Cas No. 159899-65-7 SDF
别名 肾上腺髓质素(22-52)(人体),22-52-Adrenomedullin (human)
Canonical SMILES Thr-Val-Gln-Lys-Leu-Ala-His-Gln-Ile-Tyr-Gln-Phe-Thr-Asp-Lys-Asp-Lys-Asp-Asn-Val-Ala-Pro-Arg-Ser-Lys-Ile-Ser-Pro-Gln-Gly-Tyr-NH2
分子式 C159H252N46O48 分子量 3576.04
溶解度 DMSO : 100 mg/mL (27.96 mM; Need ultrasonic); H2O : 50 mg/mL (13.98 mM; Need ultrasonic) 储存条件 Store at -20°C
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1 mM 0.2796 mL 1.3982 mL 2.7964 mL
5 mM 0.0559 mL 0.2796 mL 0.5593 mL
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