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Aftin-4 Sale

目录号 : GC31259

An inducer of Aβ42

Aftin-4 Chemical Structure

Cas No.:866893-90-5

规格 价格 库存 购买数量
10mM (in 1mL DMSO)
¥949.00
现货
5mg
¥1,170.00
现货
10mg
¥1,890.00
现货
25mg
¥3,780.00
现货
50mg
¥6,750.00
现货
100mg
¥11,700.00
现货

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Sample solution is provided at 25 µL, 10mM.

Description

Aftin-4 is an inducer of amyloid-β (1-42) (Aβ42).1 It selectively increases extracellular Aβ42 over Aβ40 production in N2a-AβPP695 cells when used at concentrations ranging from 1 to 100 mM. In vivo, aftin-4 (3-20 nmol/animal, i.c.v.) increases hippocampal Aβ42 content, lipid peroxidation, and production of the pro-inflammatory cytokines IL-1β, IL-6, and TNF-α in mice.2 It also induces spatial working and spatial reference memory deficits in mice, effects that are reversed by co-administration of the γ-secretase inhibitor BMS-299,897.

1.Hochard, A., Oumata, N., Bettayeb, K., et al.Aftins increase amyloid-β42, lower amyloid-β38, and do not alter amyloid-β40 extracellular production in vitro: Toward a chemical model of Alzheimer's disease?J. Alzheim. Dis.35(1)107-120(2013) 2.Meunier, J., Borjini, N., Gillis, C.N., et al.Brain toxicity and inflammation induced in vivo in mice by the amyloid-β forty-two inducer aftin-4, a roscovitine derivativeJ. Alzheim. Dis.44(2)507-524(2015)

实验参考方法

Animal experiment:

Mice[1]Male Swiss OF-1 mice, aged 7-9 weeks and weighing 32±2 g are used. Aftin-4 is solubilized in DMSO at a concentration of 3 mg/mL and stored at -20°C until use. Aftin-4 is administered intracerebroventricularly (i.c.v. ), with a Hamilton microsyringe equipped with a 3-mm needle in a final volume of 3 μL per mouse. The injection coordinates are -0.4 mm with respect to bregma, 1.00mm to the right from the central, and 2.50mm in depth. Aftin-4 is also injected intraperitoneally (i.p.). Aftin-4 is solubilized in DMSO 40% in distilled water at 2, 6 or 20 mg/mL and administered in a final volume of 100 μL/20 g body weight[1].

References:

[1]. Meunier J, et al. Brain toxicity and inflammation induced in vivo in mice by the amyloid-β forty-two inducer aftin-4, a roscovitine derivative. J Alzheimers Dis. 2015;44(2):507-24.

化学性质

Cas No. 866893-90-5 SDF
Canonical SMILES CC[C@@H](NC1=NC(N(C)CC2=CC=CC=C2)=C3N=CN(C(C)C)C3=N1)CO
分子式 C20H28N6O 分子量 368.48
溶解度 DMSO : ≥ 250 mg/mL (678.46 mM) 储存条件 Store at -20°C
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储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
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溶解性数据

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1 mg 5 mg 10 mg
1 mM 2.7139 mL 13.5693 mL 27.1385 mL
5 mM 0.5428 mL 2.7139 mL 5.4277 mL
10 mM 0.2714 mL 1.3569 mL 2.7139 mL
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