Alantolactone
(Synonyms: 土木香内酯; (+)-Alantolactone; Alant camphor; Inula camphor) 目录号 : GN10705
A sesquiterpene lactone with diverse biological activities
Cas No.:546-43-0
Sample solution is provided at 25 µL, 10mM.
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Alantolactone is a selective STAT3 inhibitor, with potent anticancer activity.
Alantolactone induces apoptosis in HepG2 cells in a dose-dependent manner. This Alantolactone-induced apoptosis is found to be associated with GSH depletion, inhibition of STAT3 activation, ROS generation, mitochondrial transmembrane potential dissipation, and increased Bax/Bcl-2 ratio and caspase-3 activation[1]. Alantolactone decreases STAT3 translocation to the nucleus, its DNA-binding, and STAT3 target gene expression. Alantolactone significantly inhibits STAT3 activation with a marginal effect on MAPKs and on NF-κB transcription; however, this effect is not mediated by inhibiting STAT3 upstream kinases[2].
It is found that the average tumor volume in the Alantolactone-treated mice is approximately 2.17-fold lower compared with that in the control mice. However the administration of Alantolactone does not affect the overall bodyweight during the experimental period, suggesting no apparent toxicity. Additionally, the average tumor weight is significantly lower in the Alantolactone-treated mice compared with the control mice. What’s more, the administration of Alantolactone results in a significant decrease in p-STAT3 and cyclin D1 expression in the tumor tissues[2].
References:
[1]. Khan M, et al. Alantolactone induces apoptosis in HepG2 cells through GSH depletion, inhibition of STAT3 activation, and mitochondrial dysfunction. Biomed Res Int. 2013;2013:719858.
[2]. Chun J, et al. Alantolactone selectively suppresses STAT3 activation and exhibits potent anticancer activity in MDA-MB-231 cells. Cancer Lett. 2015 Feb 1;357(1):393-403.
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Cell experiment: |
Cells are cultured for 24 h before drug treatment in 96 well plates. Cells were treated with Alantolactone (0, 10, 20, 30, 40, 50, and 60 μM) for 12 h and cell viability is measured by MTT assay[1]. |
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Animal experiment: |
Mice[2]Female athymic BALB/c nude mice at the age of 6 weeks are used. MDA-MB-231 cells (5×106 cells/200 μL) are subcutaneously injected into the right flanks of the mice. Ten days after the injection of cells, mice are randomly divided into treatment and control groups (n=5). The animals are administered Alantolactone (2.5 mg/kg of body weight, suspended in DMSO 0.1% v/v, 100 μL i.p. injection) every 2 days, whereas control animals are treated with an equal volume of saline[2]. |
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References: [1]. Khan M, et al. Alantolactone induces apoptosis in HepG2 cells through GSH depletion, inhibition of STAT3 activation, and mitochondrial dysfunction. Biomed Res Int. 2013;2013:719858. |
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| Cas No. | 546-43-0 | SDF | |
| 别名 | 土木香内酯; (+)-Alantolactone; Alant camphor; Inula camphor | ||
| 化学名 | (3aR,5S,8aR,9aR)-5,8a-dimethyl-3-methylidene-5,6,7,8,9,9a-hexahydro-3aH-benzo[f][1]benzofuran-2-one | ||
| Canonical SMILES | CC1CCCC2(C1=CC3C(C2)OC(=O)C3=C)C | ||
| 分子式 | C15H20O2 | 分子量 | 232.31 |
| 溶解度 | ≥ 23.2mg/mL in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.3046 mL | 21.523 mL | 43.0459 mL |
| 5 mM | 0.8609 mL | 4.3046 mL | 8.6092 mL |
| 10 mM | 0.4305 mL | 2.1523 mL | 4.3046 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet