Alkaline Phosphatase Bovine
目录号 : GP21429Alkaline Phosphatase Bovine Intestinal
Sample solution is provided at 25 µL, 10mM.
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The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity.
| Purity | 95% pure by Gel Filtration. | Source | Calf Intestine. |
| Phycical Appearance | Sterile Filtered clear colorless solution. | Shipping Condition | Shipped with Ice Packs. |
| Synonyms | EC 3.1.3.1; IAP; AP; ALPI; Intestinal-type alkaline phosphatase; Intestinal alkaline phosphatase. | ||
| Stability | AP should be stored at 4°C . Please Do-Not freeze. | ||
| Biological Activity | >1500 U/mg (pH 9.6), 25°C and 0.025M glycin, 10% glycerol as buffer. | ||
| Formulation | 50% glycerol, 5mM MgCl2, 0.1mM ZnCl2 and 5mM TRIS, pH 7.0. | ||
>1500 U/mg (pH 9.6), 25°C and 0.025M glycin, 10% glycerol as buffer.
AP should be stored at 4°C . Please Do-Not freeze.