Alkynyl-biotin
(Synonyms: 生物素-PEG2-C4-炔) 目录号 : GC18562
A biotin with a terminal alkyne group
Cas No.:1011268-28-2
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Alkynyl-biotin is a form of biotin with a terminal alkyne group separated by two polyethylene glycol moieties. It is used to add biotin to other molecules bearing an azide group using a copper Cu(I)-catalyzed azide-alkyne cycloaddition reaction. Alkynyl-biotin has been used to add a biotin tag to a range of biochemicals, amino acids, proteins, and nucleotides that have been modified by the addition of an azide group. Alkynyl-biotin can also be used to capture sulfenic acid-modified proteins that have been tagged with the cell-permeable probe DAz-2 .
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.134 mL | 10.6701 mL | 21.3402 mL |
| 5 mM | 0.4268 mL | 2.134 mL | 4.268 mL |
| 10 mM | 0.2134 mL | 1.067 mL | 2.134 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。