α-MSH

Name: α-MSH
SKU: GC14374
Availability: InStock
Rating: 5 (30 reviews)

(Synonyms: 美拉诺坦,α-Melanocyte-Stimulating Hormone, a-MSH) 目录号 : GC14374

α -黑色素细胞刺激激素(α-MSH)是一种具有免疫调节特性的神经肽,由原黑素皮质素(POMC)翻译后加工产生。

α-MSH Chemical Structure

Cas No.:581-05-5

规格价格库存购买数量

1 mg	¥350.00	现货
5 mg	¥1,050.00	现货
10 mg	¥1,820.00	现货
25 mg	¥3,920.00	现货

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Customer Reviews

Based on customer reviews.

Sample solution is provided at 25 µL, 10mM.

客户使用产品发表文献 1 篇

Int J Mol Sci 23.22 (2022): 14182.PMID:36430661

产品文档

Quality Control & SDS

View current batch:

Purity: >98.00%

实验参考方法

Cell experiment [1]:
Cell lines	B16 melanoma cells
Preparation Method	Cells treated with α-MSH in culture dishes (60 mm) were incubated for 72 h at 37℃ in a CO2 chamber.
Reaction Conditions	1 µM; 72h
Applications	α-MSH- stimulates melanin production in B16 melanoma cells.
Animal experiment [2]:
Animal models	Three-week old C57BL/6J male mice
Preparation Method	Mice were injected either PBS or α-MSH (500 nM) intraperitoneally once a day for two days before dark cycle. Inguinal adipose tissue (iWAT) was removed at 0.5 h, 1 h, 2 h, 4 h and 6 h after the last injection from the treatment group.
Dosage form	500 nM ; i.p;0-6 h
Applications	α-MSH promotes fatty acid oxidation in white (WAT) and brown adipose tissue (BAT).
References: [1]. Song HS, Sim SS. Acteoside inhibits alpha-MSH-induced melanin production in B16 melanoma cells by inactivation of adenyl cyclase. J Pharm Pharmacol. 2009 Oct;61(10):1347-51. doi: 10.1211/jpp/61.10.0011. PMID: 19814867. [2]. Gan L, Liu Z, et,al. α-MSH and Foxc2 promote fatty acid oxidation through C/EBPβ negative transcription in mice adipose tissue. Sci Rep. 2016 Nov 7;6:36661. doi: 10.1038/srep36661. PMID: 27819350; PMCID: PMC5098202.

产品描述

Alpha-melanocyte-stimulating hormone (α-MSH) is a neuropeptide with immunomodulatory properties that is produced by post-translational processing of proproiomelanocortin (POMC). α-MSH binds to the MC1R receptor on melanocytes to stimulate melanin secretion, promote the formation of melanocyte dendrites, and protect melanocytes. α-MSH has an effective anti-inflammatory effect on immune cells. Moreover, α-MSH was shown to inhibit IFN-γ production in antigen-stimulated T cells[1-3].α-MSH- stimulates(1 µM; 72h) melanin production in B16 melanoma cells[4].α-MSH(0-10 ng/mL; 5-10min) enhances the role of protective responses under stress conditions such as UVR by increasing melanin production and reducing mitochondria-mediated apoptosis[5]. α-MSH(100 nM; 48 h) increased tyrosinase activity and melanin content by 56.5% and 61.7%, respectively, and α-MSH changed the level of metabolites in melanoma cells[6].

α-MSH (1mg / kg; i.v;12 h) can increase bacterial load in middle cerebral artery occlusion (MCAO) mice[7]. α-MSH(500 nM ; i.p;0-6 h) promotes fatty acid oxidation in WAT and BAT[8].

References:
[1]. Ichiyama T, Sato S, et,al. The neuroimmunomodulatory peptide alpha-MSH. Ann N Y Acad Sci. 2000;917:221-6. doi: 10.1111/j.1749-6632.2000.tb05386.x. PMID: 11268347.
[2]. Li C, Wu M, et,al. α- MSH Plays Anti-inflammatory and Anti-fungal Role in Aspergillus Fumigatus Keratitis. Curr Eye Res. 2022 Mar;47(3):343-351. doi: 10.1080/02713683.2021.2006235. Epub 2021 Dec 12. PMID: 34766863.
[3]. Schulte-HerbrÜggen O, Quarcoo D, et,al. Alpha-MSH promotes spontaneous post-ischemic pneumonia in mice via melanocortin-receptor-1. Exp Neurol. 2008 Apr;210(2):731-9. doi: 10.1016/j.expneurol.2008.01.006. Epub 2008 Jan 19. PMID: 18304533.
[4]. Song HS, Sim SS. Acteoside inhibits alpha-MSH-induced melanin production in B16 melanoma cells by inactivation of adenyl cyclase. J Pharm Pharmacol. 2009 Oct;61(10):1347-51. doi: 10.1211/jpp/61.10.0011. PMID: 19814867.
[5]: Choudhary R, Sharma A, et,al. Role of alpha-melanocyte stimulating hormone (α-MSH) in modulating the molecular mechanism adopted by melanocytes of Bos indicus under UVR stress. Mol Cell Biochem. 2020 Feb;465(1-2):141-153. doi: 10.1007/s11010-019-03674-w. Epub 2019 Dec 10. PMID: 31823188.
[6]. Seo SH, Jo JK, et,al. Metabolomics Reveals the Alteration of Metabolic Pathway by Alpha-Melanocyte-Stimulating Hormone in B16F10 Melanoma Cells. Molecules. 2020 Jul 26;25(15):3384. doi: 10.3390/molecules25153384. PMID: 32722640; PMCID: PMC7436294.
[7]. Schulte-HerbrÜggen O, Quarcoo D, et,al. Alpha-MSH promotes spontaneous post-ischemic pneumonia in mice via melanocortin-receptor-1. Exp Neurol. 2008 Apr;210(2):731-9. doi: 10.1016/j.expneurol.2008.01.006. Epub 2008 Jan 19. PMID: 18304533.
[8]. Gan L, Liu Z, et,al. α-MSH and Foxc2 promote fatty acid oxidation through C/EBPβ negative transcription in mice adipose tissue. Sci Rep. 2016 Nov 7;6:36661. doi: 10.1038/srep36661. PMID: 27819350; PMCID: PMC5098202.

α -黑色素细胞刺激激素(α-MSH)是一种具有免疫调节特性的神经肽,由原黑素皮质素(POMC)翻译后加工产生。α-MSH结合黑素细胞上的MC1R受体,刺激黑色素分泌,促进黑素细胞树突的形成,起到保护黑素细胞的作用。α-MSH对免疫细胞具有有效的抗炎作用。此外,α-MSH被证明可以抑制抗原刺激T细胞中IFN-γ的产生[1-3]。

α-MSH-刺激(1 µM; 72h)B16黑色素瘤细胞的黑色素生成[4]。α-MSH(0-10 ng/mL; 5-10min)通过增加黑色素生成和减少线粒体介导的细胞凋亡来增强应激条件下(如UVR)保护反应的作用[5]。α-MSH(100 nM; 48 h)使酪氨酸酶活性和黑色素含量分别提高了56.5%和61.7%,α-MSH改变了黑色素瘤细胞代谢产物的水平[6]。

α-MSH (1mg / kg; i.v;12 h)可增加MCAO小鼠细菌负荷[7]。α-MSH(500 nM ; i.p;0-6 h)促进WAT和BAT的脂肪酸氧化[8]。

Chemical Properties

Cas No.	581-05-5	SDF
别名	美拉诺坦,α-Melanocyte-Stimulating Hormone, a-MSH
化学名	(4S,5Z,7S,8Z,10S,11Z,13S,14Z,16S,17Z,20Z,22S)-7-((1H-imidazol-5-yl)methyl)-16-((1H-indol-3-yl)methyl)-26-amino-10-benzyl-13-(3-guanidinopropyl)-5,8,11,14,17,20-hexahydroxy-22-((S)-2-((Z)-hydroxy(((S)-1-hydroxy-1-imino-3-methylbutan-2-yl)imino)methyl)pyrro
Canonical SMILES	CC([C@@](/N=C(O)/[C@]1([H])CCCN1C([C@](/N=C(O)/C/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/C)([H])CO)([H])CC2=CC=C(O)C=C2)([H])CO)([H])CCSC)([H])CCC(O)=O)([H])CC3=CN=CN3)([H
分子式	C₇₇H₁₀₉N₂₁O₁₉S	分子量	1664.9
溶解度	25mg/ml in Water	储存条件	Desiccate at -20°C
General tips	请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。
Shipping Condition	评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。

溶解性数据

制备储备液
		1 mg	5 mg	10 mg
	1 mM	0.6006 mL	3.0032 mL	6.0064 mL
	5 mM	0.1201 mL	0.6006 mL	1.2013 mL
	10 mM	0.0601 mL	0.3003 mL	0.6006 mL

摩尔浓度计算器
稀释计算器
分子量计算器

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动物体内配方计算器 (澄清溶液)

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方）

% DMSO+ % + % Tween 80+ % saline

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计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系GLPBIO）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。