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ALPL Mouse

目录号 : GP21399

Alkaline Phosphatase Liver/Bone/Kidney Mouse Recombinant

ALPL Mouse Chemical Structure

规格 价格 库存 购买数量
1μg
¥840.00
5-10工作日
5μg
¥2,030.00
5-10工作日
50μg
¥16,800.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

Description

ALPL produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 493 amino acids (19-503 a.a.) andhaving a molecular mass of 54.5kDa (Molecular size on SDS-PAGE will appear atapproximately 50-70kDa). ALPL is expressed with an 8 amino acid His tag atC-Terminus and purified by proprietary chromatographic techniques.

Product Data

Purity Greaterthan 95.0% as determined by SDS-PAGE. Source Sf9, Baculovirus cells.
Phycical Appearance Sterile Filtered colorless solution. Shipping Condition Shipped with Ice Packs.
Synonyms Alpl;Akp-2; Akp2; ALP; APTNAP; TNAP; TNSALP; HOPS; AP-TNAP; Alkaline phosphatase 2.
Amino Acid Sequence VPEKERDPSY WRQQAQETLK NALKLQKLNT NVAKNVIMFL GDGMGVSTVTAARILKGQLH HNTGEETRLE MDKFPFVALS KTYNTNAQVP DSAGTATAYL CGVKANEGTV GVSAATERTRCNTTQGNEVT SILRWAKDAG KSVGIVTTTR VNHATPSAAY AHSADRDWYS DNEMPPEALS QGCKDIAYQLMHNIKDIDVI MGGGRKYMYP KNRTDVEYEL DEKARGTRLD GLDLISIWKS FKPRHKHSHY VWNRTELLALDPSRVDYLLG LFEPGDMQYE LNRNNLTDPS LSEMVEVALR ILTKNLKGFF LLVEGGRIDH GHHEGKAKQALHEAVEMDQA IGKAGAMTSQ KDTLTVVTAD HSHVFTFGGY TPRGNSIFGL APMVSDTDKK PFTAILYGNGPGYKVVDGER ENVSMVDYAH NNYQAQSAVP LRHETHGGED VAVFAKGPMA HLLHGVHEQN YIPHVMAYASCIGANLDHCA WAGSGLEHHH HHH.
Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Biological Activity Specific activity is > 46,000 pmol/min/ug and isdefined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphateto phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 25C.
Formulation ALPL protein solution (0.5mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol.

Introduction

AlkalinePhosphatase Liver/Bone/Kidney (Alpl) is a part of the alkaline phosphatasesfamily which comprises 4 related alkaline phosphatases. Alpl is amembrane-bound glycosylated enzyme which is not expressed in any particulartissue. Alpl takes part in skeletal mineralization.

Biological Activity

Specific activity is > 46,000 pmol/min/ug and isdefined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphateto phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 25C.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

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