Amifloxacin (Win49375)
(Synonyms: 氨氟沙星; Win49375) 目录号 : GC33997
Amifloxacin (Win49375) 是一种氟化喹诺酮羧酸类抗菌剂，对多种革兰氏阴性菌具有活性。
Cas No.:86393-37-5
Sample solution is provided at 25 µL, 10mM.
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Amifloxacin (Win49375) is a fluorinated quinolone-carboxylic acid antibacterial agent with activity against a broad range of gram-negative organisms. The MICs for 90% of isolates of representative urinary tract pathogens are 0.125μg/ml for Escherichia coli, 0.5μg/ml for Klebsiella pneumoniae, 1.0ug/ml for Proteus vulgaris, 0.5ug/ml for Citrobacter freundii, 2.0μg/ml for Pseudomonas aeruginosa, and 0.5ug/ml for Serratia marcescens [1].
Amifloxacin (Win49375), was an effective drug against experimental infections in mice when given parenterally. Amifloxacin (Win49375) was highly active by the oral route, with 50% effective doses within two- to three fold of those obtained with parenteral medication [2].
References:
[1]. Neu H C, Labthavikul P. Antibacterial activity of amifloxacin (WIN 49, 375), a new quinolone agent[J]. Diagnostic microbiology and infectious disease, 1985, 3(6): 469-478.
[2]. Cornett J B, Wagner R B, Dobson R A, et al. In vitro and in vivo antibacterial activities of the fluoroquinolone WIN 49375 (amifloxacin)[J]. Antimicrobial agents and chemotherapy, 1985, 27(1): 4-10.
Amifloxacin (Win49375) 是一种氟化喹诺酮羧酸类抗菌剂，对多种革兰氏阴性菌具有活性。 90% 的代表性尿路病原体分离株的 MIC 为大肠杆菌 0.125μg/ml，肺炎克雷伯菌 0.5μg/ml，普通变形杆菌 1.0ug/ml，弗氏柠檬酸杆菌 0.5ug/ml，弗氏柠檬酸杆菌 2.0μg/ml铜绿假单胞菌和粘质沙雷氏菌 0.5ug/ml [1].
Amifloxacin (Win49375) 是一种有效的药物，当通过胃肠外给药时，它可以有效对抗小鼠的实验性感染。阿米沙星 (Win49375) 在口服途径中具有很高的活性，50% 的有效剂量是肠胃外用药的两到三倍[2]。
| Animal experiment [1]: | |
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Animal models |
ICR female mice weighing 18 to 20 g |
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Preparation Method |
ICR female mice were inoculated intraperitoneally with 0.5 ml of the E. coli Vogel bacterial suspension and then distributed into subgroups of 10 to receive serial twofold doses of the test agents and a subgroup of 20 to serve as untreated controls. The infected mice were medicated p.o. 0.8-1.3 mg/kg or s.c. 0.2-1.6 mg/kg of Amifloxacin (Win49375). The number of survivors was determined daily for 7 days. |
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Dosage form |
0.8-1.3 mg/kg/d for 7 days, p.o.; 0.2-1.6 mg/kg/d for 7 days, s.c. |
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Applications |
Amifloxacin (Win49375) showed a consistently high protective effect against bacterial infection, with ED50s of 1.0 mg/kg for p.o. and 1.0 mg/kg for s.c. . |
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References: [1]: Cornett J B, Wagner R B, Dobson R A, et al. In vitro and in vivo antibacterial activities of the fluoroquinolone WIN 49375 (amifloxacin)[J]. Antimicrobial agents and chemotherapy, 1985, 27(1): 4-10. | |
| Cas No. | 86393-37-5 | SDF | |
| 别名 | 氨氟沙星; Win49375 | ||
| Canonical SMILES | O=C(C1=CN(NC)C2=C(C=C(F)C(N3CCN(C)CC3)=C2)C1=O)O | ||
| 分子式 | C16H19FN4O3 | 分子量 | 334.35 |
| 溶解度 | H2O : 50 mg/mL (149.54 mM; ultrasonic and adjust pH to 12 with NaOH) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.9909 mL | 14.9544 mL | 29.9088 mL |
| 5 mM | 0.5982 mL | 2.9909 mL | 5.9818 mL |
| 10 mM | 0.2991 mL | 1.4954 mL | 2.9909 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet