AMP PNP
(Synonyms: 腺苷-5'-(Β,Γ-亚氨基)三磷酸四锂,Adenylyl imidodiphosphate) 目录号 : GC50334AMP PNP (adenylyl-imidodiphosphate)是ATP的非水解类似物。
Cas No.:72957-42-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
AMP PNP (adenylyl-imidodiphosphate) is a non-hydrolyzable analog of ATP. It can inhibit fast axonal transport and promote interactions between membranous organelles and microtubules [1]. AMP-PNP acts as a competitive inhibitor for most ATP-dependent systems, inhibiting a variety of ATP-hydrolyzing enzymes such as DNA topoisomerase II, SV40 large T antigen helicase, and several kinases. It also blocks ATP-sensitive, calcium-dependent potassium channels [2-4]. AMP-PNP can be used for the preparation of nucleotide solutions [5], dual-polarization fluorescence detection, actin cytoskeleton preparation for flow cells [6], and cascade binding reactions [7]. However, AMP-PNP is highly unstable under acidic conditions and rapidly hydrolyzes into phosphamide and inorganic phosphate.
References:
[1]. Brady ST. A novel brain ATPase with properties expected for the fast axonal transport motor. Nature. 1985 Sep 5-11;317(6032):73-5. doi: 10.1038/317073a0. PMID: 2412134.
[2]. Hehl S, Neumcke B. KATP channels of mouse skeletal muscle: mechanism of channel blockage by AMP-PNP. Eur Biophys J. 1994;23(4):231-7. doi: 10.1007/BF00213573. PMID: 7805625.
[3]. Subramanian R, Gelles J. Two distinct modes of processive kinesin movement in mixtures of ATP and AMP-PNP. J Gen Physiol. 2007 Nov;130(5):445-55. doi: 10.1085/jgp.200709866. PMID: 17968024; PMCID: PMC2151671.
[4]. Lasek RJ, Brady ST. Attachment of transported vesicles to microtubules in axoplasm is facilitated by AMP-PNP. Nature. 1985 Aug 15-21;316(6029):645-7. doi: 10.1038/316645a0. PMID: 4033761.
[5]. Fendley GA, Urbatsch IL, et,al. Nucleotide dependence of the dimerization of ATP binding cassette nucleotide binding domains. Biochem Biophys Res Commun. 2016 Nov 11;480(2):268-272. doi: 10.1016/j.bbrc.2016.10.046. Epub 2016 Oct 17. PMID: 27765627.
[6]. DeBerg HA, Blehm BH, et,al. Motor domain phosphorylation modulates kinesin-1 transport. J Biol Chem. 2013 Nov 8;288(45):32612-32621. doi: 10.1074/jbc.M113.515510. Epub 2013 Sep 26. PMID: 24072715; PMCID: PMC3820893.
[7]. Jung C, Hawkins JA, et,al. Massively Parallel Biophysical Analysis of CRISPR-Cas Complexes on Next Generation Sequencing Chips. Cell. 2017 Jun 29;170(1):35-47.e13. doi: 10.1016/j.cell.2017.05.044. PMID: 28666121; PMCID: PMC5552236.
AMP PNP (adenylyl-imidodiphosphate)是ATP的非水解类似物。它可抑制快速轴突转运，促进膜性细胞器和微管之间的相互作用[1]。AMP-PNP是大多数ATP性依赖系统的竞争性抑制剂，它可抑制一系列需要水解ATP的酶，比如DNA拓扑异构酶Ⅱ，SV40大T抗原螺旋酶和一系列激酶。阻断ATP敏感钙依赖性钾离子通道[2-4]。AMP-PNP可用于制备核苷酸溶液[5]，双动力荧光检测，结合肌动蛋白制备流动细胞[6]以及级联结合反应[7]。但是AMP-PNP在酸性条件下非常不稳定，会快速水解成磷酸酰胺和无机磷酸盐。
| Cas No. | 72957-42-7 | SDF | |
| 别名 | 腺苷-5'-(Β,Γ-亚氨基)三磷酸四锂,Adenylyl imidodiphosphate | ||
| Canonical SMILES | NC1=C2C(N([C@@H]3O[C@H](COP(OP(NP([O-])([O-])=O)([O-])=O)([O-])=O)[C@@H](O)[C@H]3O)C=N2)=NC=N1.[Li+].[Li+].[Li+].[Li+] | ||
| 分子式 | C10H13Li4N6O12P3 | 分子量 | 529.93 |
| 溶解度 | 26.5mg/ml in water | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.887 mL | 9.4352 mL | 18.8704 mL |
| 5 mM | 0.3774 mL | 1.887 mL | 3.7741 mL |
| 10 mM | 0.1887 mL | 0.9435 mL | 1.887 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。