Anacardic acid
(Synonyms: 漆树酸; Hydroginkgolic acid; Ginkgolic Acid C15) 目录号 : GC17284
A histone acetyltransferase inhibitor
Cas No.:16611-84-0
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Cell experiment [1]: | |
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Cell lines |
LNCaP cells |
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Preparation method |
The solubility of this compound in DMSO is >17.5 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
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Reacting condition |
25 and 125 μmol/L |
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Applications |
In LNCaP cells, Anacardic acid (AA) significantly inhibited cell proliferation. Anacardic acid induced G1/S cell cycle arrest of LNCaP cells. Cells at G0 /G1 stages sharply increased after treating LNCaP cells with 125 μmol/L Anacardic acid for 24 hours. The proportion of late apoptotic cells at 24 hours following Anacardic acid incubation increased significantly. Anacardic acid down-regulated AR through supressing p300. Anacardic acid up-regulated p53 through phosphorylation of p53 on Ser15. |
| Animal experiment [2]: | |
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Animal models |
BALB/c mice with diesel exhaust particle- (DEP-) induced lung inflammation |
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Dosage form |
Oral administration, 50, 150, or 250 mg/kg, 30 days |
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Application |
In a mice model of diesel exhaust particle- (DEP-) induced lung inflammation, pretreatment with 50, 150, or 250 mg/kg of anacardic acids (p.o.) for 30 days ameliorated antioxidant enzyme activities and decreased vascular adhesion molecule in vessels. Animals that received 50 mg/kg of anacardic acids showed decreased levels of neutrophils and tumor necrosis factor in the lungs and BALF, respectively. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1]. Tan J, Chen B, He L, et al. Anacardic acid (6-pentadecylsalicylic acid) induces apoptosis of prostate cancer cells through inhibition of androgen receptor and activation of p53 signaling[J]. Chinese Journal of Cancer Research, 2012, 24(4): 275-283. [2]. Carvalho A L N, Annoni R, Torres L H L, et al. Anacardic acids from cashew nuts ameliorate lung damage induced by exposure to diesel exhaust particles in mice[J]. Evidence-Based Complementary and Alternative Medicine, 2013, 2013. | |
IC50: Noncompetitively inhibit histone acetyltransferase (HAT) activity in prostate cancer with an IC50 value of about 5.0 M.
Anacardic acid (AA) is commonly regarded as a non-specific HAT inhibitor of p300. Meanwhile, it regulates the activity and expression of several other crucial enzymes including NFκB kinase, lipoxygenase (LOX-1), xanthine oxidase, tyrosinase and ureases. Therefore, this compound exerts anti-oxidation, anti-inflammation and anti-tumor activities in vitro and in vivo. [1]
In vitro: LNCaP, a classical metastatic prostate adenocarcinoma cell line, was adopted to study the effect of AA on cell growth, cycles and apoptosis. It was found that 125 M AA significantly inhibited LNCaP cell proliferation. In addition, the G1/S cell cycles arrest and the apoptosis of LNCaP cell was induced. Further mechanistic study suggested that AA induced cell apoptosis via suppressing p300. [1]
In vivo: Diesel exhaust particle- (DEP-) induced lung inflammation model was established to study the effect of AA on inflammation in mice. Ten days before DEP-instillation stimulation, mice were orally pretreated with 50, 150, or 250 mg/kg of AA for thirty days. All doses of AA ameliorated activities of oxidative enzymes. Moreover, 50 mg/kg of AA significantly decreased the expression level of tumor necrosis factor in lung. [2]
Clinical trial: So far, no clinical study has been conducted yet.
References:
[1] Tan J, Chen B, He L, Tang Y, Jiang Z, Yin G, Wang J, Jiang X. Anacardic acid (6-pentadecylsalicylic acid) induces apoptosis of prostate cancer cells through inhibition of androgen receptor and activation of p53 signaling. Chin J Cancer Rea. 2012 Dec; 24(4): 275–83.
[2] Carvalho A, Annoni R, Torres L, Durao A, Shimada A, Almeida F, Hebeda C, Lopes F, Dolhnikoff F, Martins M, Silva L, Farsky S, Saldiva P, Ulrich C, Owen R, Marcourakis T, Trevisan M, Mauad T. Anacardic acid from Cashew nuts ameliorate lung damage induced by exposure to exhaust particles in mice. Evid-Based Compl Alt. 2013 Jan. DOI: org/10.1155/2013/549879.
| Cas No. | 16611-84-0 | SDF | |
| 别名 | 漆树酸; Hydroginkgolic acid; Ginkgolic Acid C15 | ||
| 化学名 | 2-hydroxy-6-pentadecylbenzoic acid | ||
| Canonical SMILES | CCCCCCCCCCCCCCCC1=C(C(=CC=C1)O)C(=O)O | ||
| 分子式 | C22H36O3 | 分子量 | 348.52 |
| 溶解度 | ≥ 17.45mg/mL in DMSO, ≥ 83.8 mg/mL in EtOH | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.8693 mL | 14.3464 mL | 28.6928 mL |
| 5 mM | 0.5739 mL | 2.8693 mL | 5.7386 mL |
| 10 mM | 0.2869 mL | 1.4346 mL | 2.8693 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。