Anisomycin
(Synonyms: 茴香霉素; Flagecidin; Wuningmeisu C) 目录号 : GC11559
Anisomycin是一种从灰链霉菌中分离出来的抗生素，也是一种JNK激活剂。
Cas No.:22862-76-6
Sample solution is provided at 25 µL, 10mM.
Anisomycin, an antibiotic isolated from Streptomyces griseus, is also a JNK activator. Anisomycin inhibited protein synthesis mainly by inhibiting ribosomal subunit peptidyl transferase activity at 80S. Additionally, anisomycin induces apoptosis in various cell types through the activation of the P38, JNK, and ERK1/2 pathways, making it a promising chemotherapeutic drug for cancer[1-4].
Anisomycin(10-100nM; 24h) reduces cell viability in a concentration-dependent manner in B16 melanoma cells[5]. Anisomycin(1-80 ng/ml; 24h) may activate the P53/P21/P27 signaling pathway to reduce the expression of ICBP90, inhibit the expression of P-CDK2 to block cells in the S and G2/M phases, and ultimately lead to the inhibition of Jurkat T cell proliferation [6]. Anisomycin(31.8 μM) inhibits the proliferation, migration, and angiogenesis of OCSCs by altering the expression of antisense RNA NCBP2-AS2[7].
Anisomycin(5 mg/kg; i.p; 3 weeks) effectively disrupts breast cancer angiogenesis by impairing capillary network formation, inhibiting cell migration, proliferation, and survival in MD-MBA-231 cell-based breast cancer models[8]. Anisomycin(2-200μg/kg; i.p; 30days) upregulates utrophin protein levels in mdx mouse diaphragm[9].
References：
[1]. Grollman AP. Inhibitors of protein biosynthesis. II. Mode of action of anisomycin. J Biol Chem. 1967 Jul 10;242(13):3226-33. PMID: 6027796.
[2]. Hall SS, Loebenberg D, et,al. Structure-activity relationships of synthetic antibiotic analogues of anisomycin. J Med Chem. 1983 Apr;26(4):469-75. doi: 10.1021/jm00358a003. PMID: 6834379.
[3]. Schmeits PC, Katika MR, et,al. DON shares a similar mode of action as the ribotoxic stress inducer anisomycin while TBTO shares ER stress patterns with the ER stress inducer thapsigargin based on comparative gene expression profiling in Jurkat T cells. Toxicol Lett. 2014 Jan 30;224(3):395-406. doi: 10.1016/j.toxlet.2013.11.005. Epub 2013 Nov 15. PMID: 24247028.
[4]. Slipicevic A, Øy GF, et,al. Low-dose anisomycin sensitizes melanoma cells to TRAIL induced apoptosis. Cancer Biol Ther. 2013 Feb;14(2):146-54. doi: 10.4161/cbt.22953. Epub 2012 Nov 28. PMID: 23192275; PMCID: PMC3571996.
[5]. Ushijima H, Monzaki R, et,al. Suppressive Effects of Anisomycin on the Proliferation of B16 Mouse Melanoma Cells In Vitro. Anticancer Res. 2021 Dec;41(12):6113-6121. doi: 10.21873/anticanres.15431. PMID: 34848466.
[6]. Yu C, Xing F, et,al. Anisomycin suppresses Jurkat T cell growth by the cell cycle-regulating proteins. Pharmacol Rep. 2013;65(2):435-44. doi: 10.1016/s1734-1140(13)71019-3. PMID: 23744428.
[7]. Ling L, Wen Y, et,al. Anisomycin inhibits the activity of human ovarian cancer stem cells via regulating antisense RNA NCBP2-AS2/MEK/ERK/STAT3 signaling. J Gene Med. 2024 Jan;26(1):e3571. doi: 10.1002/jgm.3571. Epub 2023 Jul 22. PMID: 37483091.
[8]. Yang W, Zhou C, et,al. Anisomycin inhibits angiogenesis, growth, and survival of triple-negative breast cancer through mitochondrial dysfunction, AMPK activation, and mTOR inhibition. Can J Physiol Pharmacol. 2022 Jul 1;100(7):612-620. doi: 10.1139/cjpp-2021-0577. Epub 2022 Jul 19. PMID: 35852219.
[9]. Hadwen J, Farooq F, et,al. Anisomycin Activates Utrophin Upregulation Through a p38 Signaling Pathway. Clin Transl Sci. 2018 Sep;11(5):506-512. doi: 10.1111/cts.12562. Epub 2018 Jun 7. PMID: 29877606; PMCID: PMC6132359.
Anisomycin是一种从灰链霉菌中分离出来的抗生素，也是一种JNK激活剂。Anisomycin主要通过抑制80S核糖体亚基肽基转移酶活性来抑制蛋白质合成。Anisomycin可通过激活P38、JNK和ERK1/2通路诱导多种细胞类型的凋亡，是一种很有前景的癌症化疗药物[1-4]。
Anisomycin(10-100nM; 24h)以浓度依赖性的方式降低B16黑色素瘤细胞的细胞活力[5]。Anisomycin(1-80 ng/ml; 24h)可激活P53/P21/P27信号通路，降低ICBP90的表达，抑制P-CDK2的表达，使细胞进入S期和G2/M期，最终抑制Jurkat T细胞的增殖[6]。Anisomycin(31.8 μM)可以通过调节反义RNA NCBP2-AS2的表达抑制OCSCs的增殖、迁移和血管生成[7]。
Anisomycin(5 mg/kg; i.p; 3 weeks)通过抑制乳腺癌小鼠MD-MBA-231细胞毛细血管网络的形成、迁移、增殖和存活，有效靶向乳腺癌血管生成[8] 。Anisomycin(2-200μg/kg;i.p; 30days)上调MDX小鼠膈肌肌营养蛋白水平[9]。
| Cell experiment [1]: | |
Cell lines | B16 cells |
Preparation Method | Cells were seeded into a 96-well microplate (6×103 cells/well). After 24 h, the medium was changed to a fresh medium containing different concentrations of anisomycin, followed by further incubation for 24 h. |
Reaction Conditions | 0-100nM; 24h |
Applications | Anisomycin concentration higher than 10 nM led to a decrease in cell viability in a concentration-dependent manner. |
| Animal experiment [2]: | |
Animal models | Six-week-old SCID mice (MD-MBA-231 cells breast cancer model) |
Preparation Method | Treatments were initialized after 1 week of tumor inoculations. Anisomycin at 5 mg/kg or 100 µL PBS was administrated via intraperitoneal injection. |
Dosage form | 5 mg/kg; i.p; 3 weeks |
Applications | Anisomycin significantly suppresses growth of MDA-MB-231 tumor in vivo. |
References: | |
| Cas No. | 22862-76-6 | SDF | |
| 别名 | 茴香霉素; Flagecidin; Wuningmeisu C | ||
| 化学名 | (2R,3S,4S)-4-hydroxy-2-(4-methoxybenzyl)pyrrolidin-3-yl acetate | ||
| Canonical SMILES | O[C@@H]1[C@H]([C@@H](CC(C=C2)=CC=C2OC)NC1)OC(C)=O | ||
| 分子式 | C14H19NO4 | 分子量 | 265.31 |
| 溶解度 | ≥ 26.5mg/mL in DMSO | 储存条件 | Store at -20°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.7692 mL | 18.8459 mL | 37.6918 mL |
| 5 mM | 0.7538 mL | 3.7692 mL | 7.5384 mL |
| 10 mM | 0.3769 mL | 1.8846 mL | 3.7692 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00%
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