Apelin-12 (human, mouse, rat, bovine) (acetate)
(Synonyms: RPRLSHKGPMPF) 目录号 : GC52427An endogenous agonist of the APJ receptor
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Apelin-12 is a 12-amino acid peptide fragment corresponding to residues 66-77 of apelin and an endogenous agonist of the apelin receptor (APJ), with roles in viral immunity, digestion, cell cycle regulation, and vasodilation.1 It induces calcium mobilization in APJ-Gαq16 cells expressing human APJ (EC50 = 0.18 nM). Apelin-12 inhibits HIV-1 and HIV-2 infection in NP-2/CD4/APJ cells (mean IC50 = 63 µg/ml).2 It inhibits or stimulates the expression of genes associated with apoptosis, mitosis, and DNA repair in IEC-6 and Caco-2 cells, respectively, when used at a concentration of 0.01 µM.3 Apelin-12 (100-1,000 nM) induces the production of nitric oxide (NO) via PI3K and Akt signaling pathways in human umbilical vein endothelial cells (HUVECs).4 In vivo, apelin-12 (20-100 µg/kg) increases gastric acid secretion, as well as stomach histamine synthesis and secretion, in rats.5 It also reduces mean arterial pressure (MAP) in rats when administered at a dose of 15 µg/kg.6
1.Giddings, A., Runyon, S., Thomas, J., et al.Development of a functional HTS assay for the APJ receptorInt. J. High Throughput Screen.139-47(2010) 2.Zou, M.X., Liu, H.Y., Haraguchi, Y., et al.Apelin peptides block the entry of human immunodeficiency virus (HIV)FEBS Lett.473(1)15-18(2000) 3.Antushevich, H., Krawczynska, A., Kapica, M., et al.Effect of apelin on mitosis, apoptosis and DNA repair enzyme OGG 1/2 expression in intestinal cell lines IEC-6 and Caco-2Folia Histochem. Cytobiol.52(1)51-59(2014) 4.Busch, R., Strohbach, A., Pennewitz, M., et al.Regulation of the endothelial apeliPJ system by hemodynamic fluid flowCell. Signal.27(7)1286-1296(2015) 5.Ohno, S., Yakabi, K., Ro, S., et al.Apelin-12 stimulates acid secretion through an increase of histamine release in rat stomachsRegul. Pept.174(1-3)71-79(2012) 6.Lee, D.K., Saldivia, V.R., Nguyen, T., et al.Modification of the terminal residue of apelin-13 antagonizes its hypotensive actionEndocrinology146(1)231-236(2005)
| Cas No. | SDF | Download SDF | |
| 别名 | RPRLSHKGPMPF | ||
| Canonical SMILES | O=C([C@H]1N(CCC1)C(CNC([C@H](CCCCN)NC([C@@H](NC([C@H](CO)NC([C@H](CC(C)C)NC([C@H](CCCNC(N)=N)NC([C@H]2N(CCC2)C([C@@H](N)CCCNC(N)=N)=O)=O)=O)=O)=O)CC3=CN=CN3)=O)=O)=O)N[C@@H](CCSC)C(N4[C@@H](CCC4)C(N[C@H](C(O)=O)CC5=CC=CC=C5)=O)=O.CC(O)=O | ||
| 分子式 | C64H103N21O14S ? XC2H4O2 | 分子量 | 1422.7 |
| 溶解度 | DMF: insol,DMSO: insol,Ethanol: insol,PBS (pH 7.2): 5 mg/ml | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.7029 mL | 3.5144 mL | 7.0289 mL |
| 5 mM | 0.1406 mL | 0.7029 mL | 1.4058 mL |
| 10 mM | 0.0703 mL | 0.3514 mL | 0.7029 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。