Arachidonic acid (Immunocytophyt)
(Synonyms: 花生四烯酸; Immunocytophyt) 目录号 : GC31725
花生四烯酸Arachidonic acid 是一种必需的不饱和脂肪酸,存在于动物和人类脂肪中,也存在于肝脏、大脑和腺体器官中。
Cas No.:506-32-1
Sample solution is provided at 25 µL, 10mM.
Arachidonic acid is an essential unsaturated fatty acid, found in animal and human fats, as well as in liver, brain and glandular organs. It is one of the phospholipids in animals. It is a precursor to the biosynthesis of prostaglandins and leukotrienes. Arachidonic acid is an important factor in cell signaling and the occurrence of inflammation[1-3].
Arachidonic acid(0-40 µM; 3min) stimulates protein tyrosine phosphorylation in vascular cells[4]. Arachidonic acid (3.3µM;24h) supports EB formation and protects EBs from Valproic acid (VA)-induced embryotoxicity[5].
Arachidonic acid (1-4mg/kg; i.g.; once daily for 3weeks) treatment has resulted in a significant down-regulation of pro-inflammatory markers as well as the COX pathway and up-regulation of 12/15-LOX has been observed in obesity mice[6].Arachidonic acid+ diet could prevent amyloid β-protein (Aβ) deposition through the alteration of amyloid precursor protein (APP) processing in Tg2576 mice[7].
References:
[1]. Tallima H, El Ridi R. Arachidonic acid: Physiological roles and potential health benefits - A review. J Adv Res. 2017 Nov 24;11:33-41. doi: 10.1016/j.jare.2017.11.004. PMID: 30034874; PMCID: PMC6052655.
[2]. Wang B, Wu L,et,al. Metabolism pathways of arachidonic acids: mechanisms and potential therapeutic targets. Signal Transduct Target Ther. 2021 Feb 26;6(1):94. doi: 10.1038/s41392-020-00443-w. PMID: 33637672; PMCID: PMC7910446.
[3]. Turolo S, Edefonti A, et,al. Role of Arachidonic Acid and Its Metabolites in the Biological and Clinical Manifestations of Idiopathic Nephrotic Syndrome. Int J Mol Sci. 2021 May 21;22(11):5452. doi: 10.3390/ijms22115452. PMID: 34064238; PMCID: PMC8196840.
[4]. Buckley BJ, Whorton AR. Arachidonic acid stimulates protein tyrosine phosphorylation in vascular cells. Am J Physiol. 1995 Dec;269(6 Pt 1):C1489-95. doi: 10.1152/ajpcell.1995.269.6.C1489. PMID: 8572178.
[5]. Nihad M, Sen U, et,al. Arachidonic acid modulates the cellular energetics of human pluripotent stem cells and protects the embryoid bodies from embryotoxicity effects in vitro. Reprod Toxicol. 2023 Sep;120:108438. doi: 10.1016/j.reprotox.2023.108438. Epub 2023 Jul 16. PMID: 37454977.
[6]. Roy S, Ripon MAR, et,al. Arachidonic acid supplementation attenuates adipocyte inflammation but not adiposity in high fat diet induced obese mice. Biochem Biophys Res Commun. 2022 Jun 11;608:90-95. doi: 10.1016/j.bbrc.2022.03.089. Epub 2022 Mar 29. PMID: 35397428.
[7]. Hosono T, Nishitsuji K, et,al. Arachidonic acid diet attenuates brain Aβ deposition in Tg2576 mice. Brain Res. 2015 Jul 10;1613:92-9. doi: 10.1016/j.brainres.2015.04.005. Epub 2015 Apr 13. PMID: 25881896.
花生四烯酸Arachidonic acid 是一种必需的不饱和脂肪酸,存在于动物和人类脂肪中,也存在于肝脏、大脑和腺体器官中。它是动物体内磷脂的一种。它是前列腺素和白三烯生物合成的前体。花生四烯酸也是细胞信号传导和炎症发生的重要因子[1-3]。
花生四烯酸(0-40 µM; 3min)刺激血管细胞中的蛋白酪氨酸磷酸化[4]。花生四烯酸 (3.3µM;24h)支持胚状体形成并保护胚状体免受丙戊酸(VA)诱导的胚胎毒性[5]。
花生四烯酸处理(1-4mg/kg; i.g.; once daily for 3weeks)导致肥胖小鼠的促炎标志物和COX通路显著下调,12/15-LOX上调[6]。花生四烯酸饮食可通过改变Tg2576小鼠的淀粉样前体蛋白(APP)加工来阻止淀粉样β蛋白Aβ沉积[7]。
| Cell experiment [1]: | |
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Cell lines |
Embryoid bodies (EBs) |
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Preparation Method |
Embryoid bodies (EBs) were seeded in 24-well plates and treated with various concentrations of Arachidonic acid alone, Valproic acid (VA) alone and VA in combination with 3.3 µM Arachidonic acid. |
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Reaction Conditions |
3.3µM;24h |
|
Applications |
Arachidonic acid supports EB formation and protects EBs from Valproic acid (VA)-induced embryotoxicity. |
| Animal experiment [2]: | |
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Animal models |
Swiss Albino male mice (feeding high-fat diets to induce obesity) |
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Preparation Method |
Mice groups were divided into three groups. Two groups of mice received two different doses of AA, which were 1 mg/kg of body weight named Low Arachidonic Acid (LAA) and 4 mg/kg of body weight named Moderate Arachidonic Acid (MAA) once daily through oral gavage, along with HFD(high-fat diets). The remaining obese group of mice was continued with HFD. |
|
Dosage form |
1-4mg/kg; i.g; once daily for 3weeks |
|
Applications |
Arachidonic acid treatment has resulted in a significant down-regulation of pro-inflammatory markers as well as the COX pathway in obesity mice. |
|
References: [1]. Nihad M, Sen U, et,al. Arachidonic acid modulates the cellular energetics of human pluripotent stem cells and protects the embryoid bodies from embryotoxicity effects in vitro. Reprod Toxicol. 2023 Sep;120:108438. doi: 10.1016/j.reprotox.2023.108438. Epub 2023 Jul 16. PMID: 37454977. |
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| Cas No. | 506-32-1 | SDF | |
| 别名 | 花生四烯酸; Immunocytophyt | ||
| Canonical SMILES | CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(O)=O | ||
| 分子式 | C20H32O2 | 分子量 | 304.47 |
| 溶解度 | DMSO : ≥ 50 mg/mL (164.22 mM);Ethanol : ≥ 50 mg/mL (164.22 mM);Water : < 0.1 mg/mL (insoluble) | 储存条件 | -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.2844 mL | 16.422 mL | 32.844 mL |
| 5 mM | 0.6569 mL | 3.2844 mL | 6.5688 mL |
| 10 mM | 0.3284 mL | 1.6422 mL | 3.2844 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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