Arbutin-d4
(Synonyms: β-Arbutin-d4) 目录号 : GC48841
An internal standard for the quantification of arbutin
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Arbutin-d4 is intended for use as an internal standard for the quantification of arbutin by GC- or LC-MS. Arbutin is a glycosylated hydroquinone that has been found in Arctostaphylos plants and has diverse biological activities, including tyrosinase inhibitory, antioxidant, and anti-inflammatory properties.1,2 It inhibits human tyrosinase activity in crude tyrosinase solution isolated from human melanocytes (IC50s = 5.7 and 18.9 mM using L-tyrosine and L-DOPA as substrates, respectively) as well as in intact melanocytes (IC50 = 0.5 mM).3 Arbutin (50 μM) inhibits hemolysis induced by the free radical generator AAPH in sheep erythrocytes and inhibits AAPH-induced decreases in cell viability in cultured human skin fibroblasts when used at concentrations greater than 125 μM.2 In an LPS-induced rat model of acute lung injury, arbutin (50 mg/kg) prevents increases in IL-1β, IL-6, and TNF-α levels in lung tissue and serum.4 Formulations containing arbutin have been used in the treatment of hyperpigmentation disorders.
1.Seo, D.-H., Jung, J.-H., Lee, J.-E., et al.Biotechnological production of arbutins (α- and β-arbutins), skin-lightening agents, and their derivativesAppl. Microbiol. Biotechnol.95(6)1417-1425(2012) 2.Takebayashi, J., Ishii, R., Chen, J., et al.Reassessment of antioxidant activity of arbutin: Multifaceted evaluation using five antioxidant assay systemsFree Radic. Res.44(4)473-478(2010) 3.Maeda, K., and Fukuda, M.Arbutin: Mechanism of its depigmenting action in human melanocyte cultureJ. Pharmacol. Exp. Ther.276(2)765-769(1996) 4.Ye, J., Guan, M., Lu, Y., et al.Arbutin attenuates LPS-induced lung injury via Sirt1/ Nrf2/ NF-κBp65 pathwayPulm. Pharmacol. Ther.5453-59(2019)
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.6193 mL | 18.0963 mL | 36.1925 mL |
| 5 mM | 0.7239 mL | 3.6193 mL | 7.2385 mL |
| 10 mM | 0.3619 mL | 1.8096 mL | 3.6193 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。