Arctigenin
(Synonyms: 牛蒡子苷元; (-)-Arctigenin) 目录号 : GN10063
Arctigenin是一种天然木脂素化合物，已证实具有抗癌活性。Arctigenin具有强效的抗氧化、抗炎和抗病毒（甲型流感病毒）活性。
Cas No.:7770-78-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Cell experiment [1]: | |
Cell lines | U87MG and T98G human glioma cell lines |
Preparation Method | The U87MG and T98G human glioma cell lines were treated with Actigenin ( 1-40μM ) for 48 h. |
Reaction Conditions | Actigenin ( 1-40μM ) ;48 h |
Applications | Arctigenin dose-dependently inhibited the growth of U87MG and T98G cells. |
| Animal experiment [2]: | |
Animal models | Female ob/ob mice modle |
Preparation Method | Female ob/ob mice were given Arctigenin (25 mg/kg;ip; twice daily ) for 22 day. |
Dosage form | Arctigenin (200 mg/kg q2d);23 day;ip. |
Applications | Arctigenin (25 mg/kg;ip;twice daily )increased insulin-stimulated AKT phosphorylation in gastrocnemius muscle and liver,but not in perirenal fat. |
References: | |
Arctigenin,a natural lignan compound,has demonstrated anticancer activities in cancer. Arctigenin exhibits potent antioxidant, anti-inflammatory and antiviral (influenza A virus) activities [3,4].
Arctigenin (40 µM;48h) inhibited the growth of U87MG and T98G cells, induced G0/G1 cell-cycle arrest and triggered cell apoptosis in glioma cells[1]. Arctigenin (10,20,40,80 and 100μmol/L;48h) possesses anti-tumor activity in bladder cancer T24 cells. At the molecular level, arctigenin was able to reduce cyclin D1 and phospho-ERK1/2 expression, but induce phospho-p38 levels[4]. Arctigenin (0,1.56,12.5,100 μM; 24h) induce apoptosis in HCC cells via mitochondria and Fas/FasL-dependent apoptotic pathways[5].
Arctigenin (25 mg/kg;ip; twice daily ) for 23 day increased insulin-stimulated AKT phosphorylation in gastrocnemius muscle and liver, but not in perirenal fat[2]. Arctigenin (50mg/kg/day;ip) for 4 day confers protection against LPS-induced lung inflammatory and oxidative damage [6].
References:
[1]. Maimaitili A, Shu Z, Cheng X, Kaheerman K, Sikandeer A, Li W. Arctigenin, a natural lignan compound, induces G0/G1 cell cycle arrest and apoptosis in human glioma cells. Oncol Lett. 2017 Feb;13(2):1007-1013
[2]. Huang SL, Yu RT, Gong J, Feng Y, Dai YL, Hu F, Hu YH, Tao YD, Leng Y. Arctigenin, a natural compound, activates AMP-activated protein kinase via inhibition of mitochondria complex I and ameliorates metabolic disorders in ob/ob mice. Diabetologia. 2012 May;55(5):1469-81.
[3]. Hayashi K, Narutaki K, Nagaoka Y, Hayashi T, Uesato S. Therapeutic effect of arctiin and arctigenin in immunocompetent and immunocompromised mice infected with influenza A virus. Biol Pharm Bull. 2010;33(7):1199-205.
[4]. Yang S, Ma J, Xiao J, Lv X, Li X, Yang H, Liu Y, Feng S, Zhang Y. Arctigenin anti-tumor activity in bladder cancer T24 cell line through induction of cell-cycle arrest and apoptosis. Anat Rec (Hoboken). 2012 Aug;295(8):1260-6.
[5]. Lu Z, Cao S, Zhou H, Hua L, Zhang S, Cao J. Mechanism of Arctigenin-Induced Specific Cytotoxicity against Human Hepatocellular Carcinoma Cell Lines: Hep G2 and SMMC7721. PLoS One. 2015 May 1;10(5):e0125727.
[6]. Zhang WZ, Jiang ZK, He BX, Liu XB. Arctigenin Protects against Lipopolysaccharide-Induced Pulmonary Oxidative Stress and Inflammation in a Mouse Model via Suppression of MAPK, HO-1, and iNOS Signaling. Inflammation. 2015 Aug;38(4):1406-14.
Arctigenin是一种天然木脂素化合物，已证实具有抗癌活性。Arctigenin具有强效的抗氧化、抗炎和抗病毒（甲型流感病毒）活性[3,4]。
Arctigenin（40 μM；48h）抑制U87MG和T98G细胞生长，诱导G0/G1细胞周期阻滞并引发胶质瘤细胞凋亡[1]。Arctigenin（10、20、40、80和100μmol/L；48h）在膀胱癌T24细胞中具有有用的抗肿瘤活性。在分子水平上，Arctigenin能够降低细胞周期蛋白D1和磷酸化ERK1/2的表达，但诱导磷酸化p38的水平[4]。Arctigenin（0、1.56、12.5、100 μM；24h）通过线粒体和Fas/FasL依赖的凋亡途径诱导HCC细胞凋亡[5]。
Arctigenin（25 mg/kg；腹腔注射；每日两次）连续 23 天可增加腓肠肌和肝脏中胰岛素刺激的 AKT 磷酸化，但不会增加肾周脂肪中的 AKT 磷酸化[2]。Arctigenin（50 mg/kg/day；腹腔注射）连续 4 天可防止 LPS 引起的肺部炎症和氧化损伤[6]。
| Cas No. | 7770-78-7 | SDF | |
| 别名 | 牛蒡子苷元; (-)-Arctigenin | ||
| 化学名 | (3R,4R)-4-[(3,4-dimethoxyphenyl)methyl]-3-[(4-hydroxy-3-methoxyphenyl)methyl]oxolan-2-one | ||
| Canonical SMILES | COC1=C(C=C(C=C1)CC2COC(=O)C2CC3=CC(=C(C=C3)O)OC)OC | ||
| 分子式 | C21H24O6 | 分子量 | 372.41 |
| 溶解度 | ≥ 17.2mg/mL in DMSO | 储存条件 | Desiccate at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.6852 mL | 13.4261 mL | 26.8521 mL |
| 5 mM | 0.537 mL | 2.6852 mL | 5.3704 mL |
| 10 mM | 0.2685 mL | 1.3426 mL | 2.6852 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。