Arphamenine B (hemisulfate)
目录号 : GC16829
An inhibitor of aminopeptidase B
Cas No.:144110-38-3
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Arphamenine B is a specific inhibitor of aminopeptidase B first isolated from bacteria [1]. Aminopeptidase B (Ap-B) is a Zn2+-dependent exopeptidase which selectively removes Arg and/or Lys residues from the N terminus of several peptide substrates. Aminopeptidase B has been involved in processing events occurring either during its intracellular transport along the secretory pathway or at the plasma membrane level [2].
In vitro: Arphamenine B inhibited the activity of aminopeptidase enzyme with an IC50 value of 9.0 μM [2]. Arphamenine B strongly inhibited transport by the oligopeptide/H+ symporter with the EC50 values of 15 to 67 μM. Arphamenine at concentration 100 μM acted as either ineffective or weak inhibitor of membrane-associated hydrolysis [4]. Arphamenine selectively suppressed dipeptide hydrolysis [4].
References:
[1] Umezawa H, AOYAGI T, OHUCHI S, et al. Arphamenines A and B, new inhibitors of aminopeptidase B, produced by bacteria[J]. The Journal of antibiotics, 1983, 36(11): 1572-1575.
[2] Balogh A, Cadel S, Foulon T, et al. Aminopeptidase B: a processing enzyme secreted and associated with the plasma membrane of rat pheochromocytoma (PC12) cells[J]. Journal of Cell Science, 1998, 111(2): 161-169.
[3] Sajid M, Isaac R E, Harrow I D. Purification and properties of a membrane aminopeptidase from Ascaris suum muscle that degrades neuropeptides AF1 and AF2[J]. Molecular and biochemical parasitology, 1997, 89(2): 225-234.
[4] Daniel H, Adibi S A. Functional separation of dipeptide transport and hydrolysis in kidney brush border membrane vesicles[J]. The FASEB journal, 1994, 8(10): 753-759.
| Cas No. | 144110-38-3 | SDF | |
| 化学名 | αR-[(3S)-3-amino-6-[(aminoiminomethyl)amino]-2-oxohexyl]-4-hydroxy-benzenepropanoic acid, hemisulfate | ||
| Canonical SMILES | O=C([C@@H](N)CCCNC(N)=N)C[C@H](C(O)=O)CC1=CC=C(O)C=C1.OS(O)(=O)=O | ||
| 分子式 | C16H24N4O4 • 1/2H2SO4 | 分子量 | 385.4 |
| 溶解度 | ≤0.2mg/ml in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.5947 mL | 12.9735 mL | 25.9471 mL |
| 5 mM | 0.5189 mL | 2.5947 mL | 5.1894 mL |
| 10 mM | 0.2595 mL | 1.2974 mL | 2.5947 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet