Atrial Natriuretic Peptide (ANP) (1-28), human, porcine
(Synonyms: 卡培立肽,Atrial Natriuretic Peptide (ANP) (1-28), human, porcine) 目录号 : GC34023
卡培肽Carperitide (Atrial Natriuretic Peptide (ANP) (1-28), human, porcine) 是一种含 28 个氨基酸的激素,由心脏产生并分泌,以响应心脏损伤和机械拉伸。
Cas No.:89213-87-6
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Animal experiment [1]: |
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Animal models |
Female beagle dogs weighing 10–14 kg |
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Protocol |
Protocol: Effects of carperitide on coronary vasodilation in nonischemic hearts. Five dogs were used. Coronary arterial and venous blood was sampled for blood gas analysis. Myocardial oxygen consumption (ml per 100 g per min) is calculated by coronary blood flow (CBF) (ml per 100 g per min) the oxygen difference between coronary arterial and venous blood (ml dl-1). Measured coronary perfusion pressure (CPP) and CBF after dogs were randomly administered carperitide at a dose of 0.025, 0.05, 0.1 or 0.2 μg kg-1 min-1 into the left anterior descending coronary artery (LAD). |
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Dosage form |
0.025, 0.05, 0.1 or 0.2 μg kg-1 min-1 |
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Applications |
CBF increased based on the dose of carperitide, with saturation of coronary vasodilation at a dose of 0.1μg kg−1 min−1despite no changes in CPP (102±2 mm Hg) or myocardial oxygen consumption. |
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References: [1]. Asanuma H, Sanada S, et,al. Carperitide induces coronary vasodilation and limits infarct size in canine ischemic hearts: role of NO. Hypertens Res. 2014 Aug;37(8):716-23. doi: 10.1038/hr.2014.70. Epub 2014 Apr 3. PMID: 24694647. |
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Atrial Natriuretic Peptide (ANP) (1-28), human, porcine (Carperitide) is a hormone containing 28 amino acids that is produced and secreted by the heart in response to cardiac injury and mechanical stretching[1]. Carperitide is widely used in patients with Heart failure(HF)[2].
Carperitide(1 mM;10min)significantly reduced infarct size in isolated rat hearts[3]. Carperitide inhibits immunoreactive (ir)-endothelin-1 secretion and increases cyclic GMP in the human umbilical-vein endothelial cells[4].
Carperitide dose increased, coronary blood flow (CBF) increased, and coronary artery diastole reached saturation at 0.1µg kg -1 min -1, but there was no change in CPP or myocardial oxygen consumption in dog[5].
References:
[1]. Kohno M, Yokokawa K, et,al. Atrial and brain natriuretic peptides inhibit the endothelin-1 secretory response to angiotensin II in porcine aorta. Circ Res. 1992 Feb;70(2):241-7. doi: 10.1161/01.res.70.2.241. PMID: 1310444.
[2]. Mukoyama M, Nakao K, et al. Brain natriuretic peptide as a novel cardiac hormone in humans. Evidence for an exquisite dual natriuretic peptide system, atrial natriuretic peptide and brain natriuretic peptide. J Clin Invest. 1991 Apr;87(4):1402-12. doi: 10.1172/JCI115146. PMID: 1849149; PMCID: PMC295184.
[3]. Okawa H, Horimoto H, et,al.Preischemic infusion of alpha-human atrial natriuretic peptide elicits myoprotective effects against ischemia reperfusion in isolated rat hearts. Mol Cell Biochem. 2003 Jun;248(1-2):171-7. doi: 10.1023/a:1024148621505. PMID: 12870670.
[4].Kohno M, Yasunari K, et,al. Inhibition by atrial and brain natriuretic peptides of endothelin-1 secretion after stimulation with angiotensin II and thrombin of cultured human endothelial cells. J Clin Invest. 1991 Jun;87(6):1999-2004. doi: 10.1172/JCI115228. PMID: 1645748; PMCID: PMC296954.
[5].Asanuma H, Sanada S, et,al.Carperitide induces coronary vasodilation and limits infarct size in canine ischemic hearts: role of NO. Hypertens Res. 2014 Aug;37(8):716-23. doi: 10.1038/hr.2014.70. Epub 2014 Apr 3. PMID: 24694647.
卡培肽Carperitide (Atrial Natriuretic Peptide (ANP) (1-28), human, porcine) 是一种含 28 个氨基酸的激素,由心脏产生并分泌,以响应心脏损伤和机械拉伸。Carperitide(重组人ANP)广泛应用于心衰患者[1-2]。
Carperitide显著降低离体大鼠心脏梗死面积[3]。Carperitide抑制ir-Endothelin-1的分泌,增加人脐静脉内皮细胞的环GMP[4].
CBF随着Carperitide剂量的增加而增加,在0.1µg kg-1 min-1剂量时冠状动脉舒张饱和,但CPP(102±2 mm Hg)或心肌耗氧量没有变化[5]。
| Cas No. | 89213-87-6 | SDF | |
| 别名 | 卡培立肽,Atrial Natriuretic Peptide (ANP) (1-28), human, porcine | ||
| Canonical SMILES | Ser-Leu-Arg-Arg-Ser-Ser-Cys-Phe-Gly-Gly-Arg-Met-Asp-Arg-Ile-Gly-Ala-Gln-Ser-Gly-Leu-Gly-Cys-Asn-Ser-Phe-Arg-Tyr (Disulfide bridge: Cys7-Cys23) | ||
| 分子式 | C127H203N45O39S3 | 分子量 | 3080.44 |
| 溶解度 | Water: 1 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.3246 mL | 1.6231 mL | 3.2463 mL |
| 5 mM | 0.0649 mL | 0.3246 mL | 0.6493 mL |
| 10 mM | 0.0325 mL | 0.1623 mL | 0.3246 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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