Auramine O
(Synonyms: 金胺) 目录号 : GC65458
Auramine O 是一种黄色荧光染料，可用于染色抗酸细菌。Auramine O 有毒性，在环境中稳定且耐腐蚀。
Cas No.:2465-27-2
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Auramine O is a yellow fluorescent dye and can be used to stain acid-fast bacteria. Auramine O is toxic and resistant in the environment[1][2].
[1]. Kommareddi S, et al. Nontuberculous mycobacterial infections: comparison of the fluorescent auramine-O and Ziehl-Neelsen techniques in tissue diagnosis. Hum Pathol. 1984 Nov;15(11):1085-9. [2]. Hosseinpour S A, et al. Use of metal composite MOF‐5‐Ag2O‐NPs as an adsorbent for the removal of Auramine O dye under ultrasound energy conditions. Applied Organometallic Chemistry, 2018, 32(2): e4007.
| Cas No. | 2465-27-2 | SDF | Download SDF |
| 别名 | 金胺 | ||
| 分子式 | C17H22ClN3 | 分子量 | 303.83 |
| 溶解度 | DMSO : 16.67 mg/mL (54.87 mM; ultrasonic and warming and heat to 60°C) | 储存条件 | Store at 2-8°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.2913 mL | 16.4566 mL | 32.9131 mL |
| 5 mM | 0.6583 mL | 3.2913 mL | 6.5826 mL |
| 10 mM | 0.3291 mL | 1.6457 mL | 3.2913 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Auramine O in foods and spices determined by an UPLC-MS/MS method
Food Addit Contam Part B Surveill 2020 Sep;13(3):171-176.PMID:32238061DOI:10.1080/19393210.2020.1742208.
Auramine O (AO) is a banned food additive and has been classified as an illegal colourant. Therefore, the presence of AO in food should be strictly monitored. In this study, a sensitive UPLC-MS/MS method was applied to monitor AO in 211 food and spice samples. The optimised separation was achieved with a mobile phase consisting of 100 mM ammonium formate at pH 2.9 and acetonitrile, reversed-phase CORTECS T3 column (2.7 µm, 2.1 × 100 mm) operated at 40ºC with a gradient time of 20.0 min (0-95% methanol) at a flow rate of 0.3 mL/min. Limit of detection (LOD) and limit of quantification (LOQ) of the method were 0.1 μg/kg and 0.5 μg/kg, respectively. The results showed that 27.0% of samples were contaminated with AO. Considering the common consumption of sour bamboo shout and turmeric powder by so many consumers, AO exposure is significant and should be decreased.
Label free detection of Auramine O by G-quadruplex-based fluorescent turn-on strategy
Spectrochim Acta A Mol Biomol Spectrosc 2022 Feb 15;267(Pt 2):120532.PMID:34776374DOI:10.1016/j.saa.2021.120532.
Auramine O (AO) is a synthetic dye used in paper and textile industries. Although it has been an unauthorized food additive in many countries due to its toxic and carcinogenic possibility, its illegal uses have been detected in certain food products such as pasta, semolina and spices and also in pharmaceuticals. The presence of AO in food products should be monitored, therefore, to minimize the negative health effects on consumers. In this study, a simple, highly sensitive and selective label free detection method was investigated for AO by G-quadruplex-based fluorescent turn-on strategy. The optimum fluorescent detection assay was achieved with a specific G-quadruplex DNA sequence, c-myc, at 400 nM in Tris-HCl buffer at pH 7.4. The linearity of fluorescence intensity depending on AO concentration ranged from 0 to 0.07 µM and LOD and LOQ were 3 nM and 10 nM, respectively. The G-quadruplex-based detection assay was highly specific for AO as compared to other two synthetic food colorings and successfully applied to determine AO in pasta, bulgur and curry powder with recoveries in the range from 70.33% to 106.49%. This G-quadruplex-based label free detection assay has a significant potential to be used in the detection of AO in food products.
Auramine O interaction with DNA: a combined spectroscopic and TD-DFT analysis
Phys Chem Chem Phys 2019 Oct 7;21(37):20606-20612.PMID:31528919DOI:10.1039/c9cp03071a.
In this work, the interaction between an Auramine O (AuO) fluorescent molecular rotor and natural DNA, its thermodynamic aspects and the resulting variation of the optical properties upon binding are addressed by a combined spectroscopic (UV-vis and fluorescence) and computational approach. DNA binding causes a shift in the maximum of absorption from 432 nm to 444 nm, a decrease of the extinction coefficient and a dramatic enhancement of fluorescence emission, these results being in agreement with intercalation into the polynucleotide helix. Intercalation is indeed confirmed by the thermodynamic parameters for the binding reaction (in particular, the highly negative ΔH). Theoretical modelling at the TD-DFT level was done on a simplified model system consisting of the AuO molecule intercalated between two DNA base pairs. The evolution of the calculated vertical transitions quantitatively reproduces the experimentally observed hypo- and bathochromic shifts, thus confirming the intercalation hypothesis.
Behavior of Auramine O in the Aqueous Solution of Two Kolliphors and Their Mixture
Molecules 2022 Dec 2;27(23):8493.PMID:36500588DOI:10.3390/molecules27238493.
The studies on the behavior of Auramine O (AuO) at the water-air interface and in the bulk phase of the aqueous solution of Kolliphor® ELP (ELP) and Kolliphor® RH 40 (RH40) and their mixture were based on the results obtained from the measurements of the contact angle of water, formamide and diiodomethane on the polytetrafluoroethylene covered by the AuO layer, the surface tension of the aqueous solution of AuO, AuO + ELP, AuO + RH40, AuO + ELP + RH40, density and fluorescence intensity. Based on the obtained results, it was possible to determine components and parameters of the AuO surface tension, concentration and composition of the mixed monolayer, including AuO, ELP and RH40, as well as that of the mixed micelles, and to determine the Gibbs standard free energy of adsorption, micellization and AuO solubilization. The obtained results also showed that surface tension isotherms of the studied solutions can be described by the Szyszkowski equation and the exponential function of the second order and predicted by the Fainerman and Miller equation. In addition, the mixed surface layer composition can be predicted based on the contribution of the components of this layer to the water surface tension reduction.
Auramine O, an incense smoke ingredient, promotes lung cancer malignancy
Environ Toxicol 2017 Nov;32(11):2379-2391.PMID:28722353DOI:10.1002/tox.22451.
Burning incense to worship deities is a popular religious ritual in large parts of Asia, and is a popular custom affecting more than 1.5 billion adherents. Due to incomplete combustion, burning incense has been well recognized to generate airborne hazards to human health. However, the correlation between burning incense and lung cancer in epidemiological studies remains controversy. Therefore, we speculated that some unknown materials in incense smoke are involved in the initiation or progression of lung cancer. Based on this hypothesis, we identified a major compound Auramine O (AuO) from the water-soluble fraction of incense burned condensate using mass spectrometry. AuO is commonly used in incense manufacture as a colorant. Due to thermostable, AuO released from burned incenses becomes an unexpected air pollutant. AuO is classified as a Group 2B chemical by the International Agency of Research on Cancer (IARC), however, the damage of AuO to the respiratory system remains elusive. Our study revealed that AuO has no apparent effect on malignant transformation; but, it dramatically promotes lung cancer malignancy. AuO accumulates in the nucleus and induces the autophagy activity in lung tumor cells. AuO significantly enhances migration and invasive abilities and the in vitro and in vivo stemness features of lung tumor cells through activating the expression of aldehyde dehydrogenase family 1 member A1 (ALDH1A1), and ALDH1A1 knockdown attenuates AuO-induced autophagy activity and blocks AuO-induced lung tumor malignancy. In conclusion, we found that AuO, an ingredient of incense smoke, significantly increases the metastatic abilities and stemness characters of lung tumor cells through the activation of ALDH1A1, which is known to be associated with poor outcome and progression of lung cancer. For public health, reducing or avoiding the use of AuO in incense is recommended.