Aurintricarboxylic Acid (ammonium salt)
(Synonyms: 铝试剂) 目录号 : GC46895
A protein synthesis inhibitor with diverse biological activities
Cas No.:569-58-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Aurintricarboxylic acid (ATA) is an inhibitor of protein synthesis that has diverse biological activities.1,2,3,4,5,6,7,8 It inhibits the activity of a variety of enzymes, including the nucleases DNase I, RNase A, and S1, as well as the serine proteases trypsin and chymotrypsin in a concentration-dependent manner.2,3 ATA inhibits replication of severe acute respiratory syndrome coronavirus (SARS-CoV; EC50 = 200 µg/ml) and human enterovirus 71 in Vero cells (EV71; EC50 = 2.9 µM), as well as reduces HIV-1- or HIV-2-induced cytopathogenicity in MT-4 cells (IC50s = 1.1 and 0.85 µg/ml, respectively).4,5,6 It inhibits apoptosis induced by sanguinarine in K562 leukemia cells when used at a concentration of 100 µM.7 ATA (20 mg/kg, i.p.) decreases CNS CD4+ T cell infiltration and reduces hind limb weakness and paralysis in a mouse model of myelin oligodendrocyte glycoprotein-induced experimental autoimmune encephalomyelitis (EAE).8
1.Siegelman, F., and Apirion, D.Aurintricarboxylic acid, a preferential inhibitor of initiation of protein synthesisJ. Bacteriol.105(3)902-907(1971) 2.Hallick, R.B., Chelm, B.K., Gray, P.W., et al.Use of aurintricarboxylic acid as an inhibitor of nucleases during nucleic acid isolationNucleic Acids Res.4(9)3055-3064(1977) 3.Bina-Stein, M., and Tritton, T.R.Aurintricarboxylic acid is a nonspecific enzyme inhibitorMol. Pharmacol.12(1)191-193(1976) 4.He, R., Adonov, A., Traykova-Adonova, M., et al.Potent and selective inhibition of SARS coronavirus replication by aurintricarboxylic acidBiochem. Biophys. Res. Commun.320(4)1199-1203(2004) 5.Hung, H.-C., Chen, T.-C., Fang, M.-Y., et al.Inhibition of enterovirus 71 replication and the viral 3D polymerase by aurintricarboxylic acidJ. Antimicrob. Chemother.65(4)676-683(2010) 6.Cushman, M., Wang, P.L., Chang, S.H., et al.Preparation and anti-HIV activities of aurintricarboxylic acid fractions and analogues: Direct correlation of antiviral potency with molecular weightJ. Med. Chem.34(1)329-337(1991) 7.Hallock, S., Tang, S.-C., Buja, L.M., et al.Aurintricarboxylic acid inhibits protein synthesis independent, sanguinarine-induced apoptosis and oncosisToxicol. Pathol.35(2)300-309(2007) 8.Zhang, F., Wei, W., Chai, H., et al.Aurintricarboxylic acid ameliorates experimental autoimmune encephalomyelitis by blocking chemokine-mediated pathogenic cell migration and infiltrationJ. Immunol.190(3)1017-1025(2013)
| Cas No. | 569-58-4 | SDF | |
| 别名 | 铝试剂 | ||
| Canonical SMILES | [O-]C(C(C(C=C/1)=O)=CC1=C(C2=CC=C(O)C(C([O-])=O)=C2)\C3=CC=C(O)C(C([O-])=O)=C3)=O.[NH4+].[NH4+].[NH4+] | ||
| 分子式 | C22H11O9.3NH4 | 分子量 | 473.4 |
| 溶解度 | DMF: 20 mg/ml,DMSO: 20 mg/ml,Ethanol: 10 mg/ml,PBS (pH 7.2): 100 μg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.1124 mL | 10.5619 mL | 21.1238 mL |
| 5 mM | 0.4225 mL | 2.1124 mL | 4.2248 mL |
| 10 mM | 0.2112 mL | 1.0562 mL | 2.1124 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Rapid, high-quality and epidermal-specific isolation of RNA from human skin
Exp Dermatol 2007 Mar;16(3):185-90.PMID:17286810DOI:10.1111/j.1600-0625.2006.00534.x.
As global transcriptome analyses with a growing demand on layer-specific applications are widely used in cutaneous biology, we investigated the effect of established and optimized dermo-epidermal separation methods on the quality of RNA. We compared enzymatic separation with dispase, chemical separation with 1 M sodium chloride and heat separation to a treatment with 3.8% ammonium thioyanate. The impact of freezing as well as the addition of 10 mM Aurintricarboxylic Acid was considered in the evaluation of the amount and quality of isolated RNA from dermis and epidermis. Using the low abundant gene kallikrein 12 for real-time PCR analysis, we were able to demonstrate the superior RNA quality after dermo-epidermal separation using 3.8% ammonium thiocyanate. In addition to the time effectiveness this separation technique promises dermal and epidermal purity and is therefore the method of choice for producing high-quality RNA for genome-wide dermal and epidermal transcriptional analysis.
Iron as a possible aggravating factor for osteopathy in itai-itai disease, a disease associated with chronic cadmium intoxication
J Bone Miner Res 1991 Mar;6(3):245-55.PMID:2035351DOI:10.1002/jbmr.5650060306.
Itai-itai disease is thought to be the result of chronic cadmium (Cd) intoxication. We examined 23 autopsy cases of itai-itai disease and 18 cases of sudden death as controls. Urine and blood samples from 10 patients were collected before they died and revealed the presence of severe anemia and renal tubular injuries. Undecalcified sections of iliac bone were stained with Aluminon reagent, and ammonium salt of Aurintricarboxylic Acid, and Prussian blue reagent in all cases of itai-itai disease. These two reagents reacted at the same mineralization fronts. X-ray microanalysis revealed the presence of iron at mineralization fronts in itai-itai disease. Five patients showed evidence of hemosiderosis in the liver, spleen, and pancreas, probably as a result of post transfusion iron overload. Renal calculi and calcified aortic walls were also stained with Prussian blue reagent in several patients. Neither ferritin nor transferrin were visualized at mineralization fronts in itai-itai disease by immunohistochemical staining. These results suggest that iron is bound to calcium or to calcium phosphate by a physicochemical reaction. A marked osteomalacia was observed in 10 cases of itai-itai disease by histomorphometry. Regression analyses of data from cases of itai-itai disease suggested that an Aluminon-positive metal inhibited mineralization and that renal tubules were injured. Since bone Cd levels were increased in itai-itai disease, it is likely that renal tubules were injured by exposure to Cd. Therefore, stainable bone iron is another possible aggravating factor for osteopathy in itai-itai disease, and a synergistic effect between iron and Cd on mineralization is proposed.
Bone marrow aluminium storage in renal failure
J Clin Pathol 1983 Nov;36(11):1288-91.PMID:6630578DOI:10.1136/jcp.36.11.1288.
Using the staining method for aluminium with the ammonium salt of aurine tricarboxylic acid, aluminon, 18 patients with end stage renal disease gave positive reactions in iliac crest bone biopsies and 11 of these had positive staining in the bone marrow. In one the marrow was positive and the bone negative. The marrow reaction is putatively regarded as caused by aluminium storage in unidentified cells, possibly of the macrophage system which are strongly fluorescent when examined after prior tetracycline labelling. Marrow storage should be considered when assessing the bone aluminium burden.