Aurothioglucose (hydrate)
(Synonyms: Gold Thioglucose) 目录号 : GC49646
A TrxR inhibitor
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >80.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Aurothioglucose is an inhibitor of thioredoxin reductase (TrxR; IC50 = 0.065 µM for the human placental enzyme).1 It is selective for TrxR over glutathione reductase and glutathione peroxidase (GPX; IC50s = >100 and 80 µM, respectively). Aurothioglucose (100 µM) inhibits IL-1β-induced production of IL-6 and IL-8 in isolated human rheumatoid synovial fibroblasts.2 It reduces HIV-1 replication induced by TNF-α in OM10.1 cells latently infected with HIV-1 when used at concentrations of 10 and 25 µM.3 Aurothioglucose (25 mg/kg) improves survival in a mouse model of acute respiratory distress syndrome (ARDS) induced by LPS and hyperoxia.4 It induces obesity and increases food intake and blood glucose levels in genetically diabetic KK mice.5 Formulations containing aurothioglucose have previously been used in the treatment of rheumatoid arthritis.
1.Gromer, S., Arscott, L.D., Williams, C.H., Jr., et al.Human placenta thioredoxin reductase. Isolation of the selenoenzyme, steady kinetics, and inhibition by therapeutic gold compoundsThe Journal of Biological Chemisty273(32)20096-20101(1998) 2.Yoshida, S., Kato, T., Sakurada, S., et al.Inhibition of IL-6 and IL-8 induction from cultured rheumatoid synovial fibroblasts by treatment with aurothioglucoseInt. Immunol.11(2)151-158(1999) 3.Traber, K.E., Okamoto, H., Kurono, C., et al.Anti-rheumatic compound aurothioglucose inhibits tumor necrosis factor-α-induced HIV-1 replication in latently infected OM10.1 and Ach2 cellsInt. Immunol.11(2)143-150(1999) 4.Britt, R.D., Jr., Velten, M., Locy, M.L., et al.The thioredoxin reductase-1 inhibitor aurothioglucose attenuates lung injury and improves survival in a murine model of acute respiratory distress syndromeAntioxid. Redox Signal.20(17)2681-2691(2014) 5.Matsuo, T., and Shino, A.Induction of diabetic alterations by goldthioglucose-obesity in KK,ICR and C57BL miceDiabetologia8(6)391-397(1972)
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.5497 mL | 12.7486 mL | 25.4972 mL |
| 5 mM | 0.5099 mL | 2.5497 mL | 5.0994 mL |
| 10 mM | 0.255 mL | 1.2749 mL | 2.5497 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。