Azidamfenicol
(Synonyms: 叠氮氯霉素) 目录号 : GC40084
A broad-spectrum chloramphenicol-like antibiotic
Cas No.:13838-08-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Azidamfenicol is a broad-spectrum chloramphenicol-like antibiotic that is active against Gram-negative and Gram-positive bacteria. It inhibits ribosomal peptidyltransferase (Ki = 22 µM).
| Cas No. | 13838-08-9 | SDF | |
| 别名 | 叠氮氯霉素 | ||
| Canonical SMILES | OC[C@@H](NC(CN=[N+]=[N-])=O)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 | ||
| 分子式 | C11H13N5O5 | 分子量 | 295.3 |
| 溶解度 | DMF: soluble,DMSO: soluble,Ethanol: soluble,Methanol: soluble | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.3864 mL | 16.9319 mL | 33.8639 mL |
| 5 mM | 0.6773 mL | 3.3864 mL | 6.7728 mL |
| 10 mM | 0.3386 mL | 1.6932 mL | 3.3864 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Bacterial Dehydrogenases Facilitate Oxidative Inactivation and Bioremediation of Chloramphenicol
Chembiochem 2023 Jan 17;24(2):e202200632.PMID:36353978DOI:10.1002/cbic.202200632.
Antimicrobial resistance represents a major threat to human health and knowledge of the underlying mechanisms is therefore vital. Here, we report the discovery and characterization of oxidoreductases that inactivate the broad-spectrum antibiotic chloramphenicol via dual oxidation of the C3-hydroxyl group. Accordingly, chloramphenicol oxidation either depends on standalone glucose-methanol-choline (GMC)-type flavoenzymes, or on additional aldehyde dehydrogenases that boost overall turnover. These enzymes also enable the inactivation of the chloramphenicol analogues thiamphenicol and Azidamfenicol, but not of the C3-fluorinated florfenicol. Notably, distinct isofunctional enzymes can be found in Gram-positive (e. g., Streptomyces sp.) and Gram-negative (e. g., Sphingobium sp.) bacteria, which presumably evolved their selectivity for chloramphenicol independently based on phylogenetic analyses. Mechanistic and structural studies provide further insights into the catalytic mechanisms of these biotechnologically interesting enzymes, which, in sum, are both a curse and a blessing by contributing to the spread of antibiotic resistance as well as to the bioremediation of chloramphenicol.
[Contact allergy to clotrimazole and Azidamfenicol]
Derm Beruf Umwelt 1987 Mar-Apr;35(2):64-6.PMID:2954808doi
A 19 year old man developed an acute contact dermatitis of the anogenital region after the application of an antimycotic cream containing clotrimazole and azida col. Patch testing revealed sensitization to both active ingredients.
Derivative spectrophotometric determination of clotrimazole in single formulations and in combination with other drugs
J Assoc Off Anal Chem 1989 May-Jun;72(3):432-5.PMID:2745365doi
First (D1) and second (D2) derivative spectrophotometric methods are presented for the determination of clotrimazole after its acid hydrolysis. Mixtures of clotrimazole with Azidamfenicol and dexamethasone have been assayed using D2 measurement at 302 nm after acid hydrolysis for clotrimazole, D1 measurement at 288 nm for Azidamfenicol, and D1 measurement at 436 nm after reaction with phenylhydrazinium sulfate for dexamethasone. Reproducible results with relative standard deviations of less than 2% are obtained. The proposed method has been successfully applied to the analysis of creams, topical solutions, and vaginal tablets.