Azidobutyric acid NHS ester

Azidobutyric acid NHS ester is a heterobifunctional crosslinker mainly used for the azido labeling of amino-containing biomolecules^[1]. The azido group (-N3) can participate in click chemistry reactions, such as reacting with alkynes, DBCO (dibenzocyclooctyne), and BCN (bicyclo[6.1.0]non-4-yne) under copper catalysis to form stable triazole rings^[2,3,4]. NHS ester (N-hydroxysuccinimide) is a commonly used reactive group for primary amines. It can react with primary amines to form stable amide bonds, facilitating the efficient labeling of target molecules^[5]. The chemical formula of Azidobutyric acid NHS ester is C8H10N4O4, with a molecular weight of 226.19. This product is for research use only and is not intended for food, drugs, medical devices, or cosmetics.

References:
[1] Von Maltzahn G, Ren Y, Park J H, et al. In vivo tumor cell targeting with “click” nanoparticles[J]. Bioconjugate chemistry, 2008, 19(8): 1570-1578.
[2] Vogele K, Frank T, Gasser L, et al. Towards synthetic cells using peptide-based reaction compartments[J]. Nature Communications, 2018, 9(1): 3862.
[3] Kuhn J, Klein P M, Al Danaf N, et al. Supramolecular assembly of aminoethylene‐lipopeptide PMO conjugates into RNA splice‐switching nanomicelles[J]. Advanced Functional Materials, 2019, 29(48): 1906432.
[4] Xiong C. Enzymatic modification of DNA and RNA 3'-termini for click ligation[D]. University of Southampton, 2014.
[5] Holmes K L, Lantz L M. Protein labeling with fluorescent probes[J]. Methods in cell biology, 2001, 63: 185-204.

叠氮丁酸NHS酯（Azidobutyric acid NHS ester）是一种异双功能交联剂（crosslinker），主要用于含氨基生物分子的叠氮基标记^[1]。叠氮基团（-N3）可以参与点击化学反应，如与炔烃（alkyne）、DBCO（二苄基环辛炔）和BCN（双环[6.1.0]壬炔）在铜催化下反应，形成稳定的三唑环^[2,3,4]。NHS酯（N-羟基琥珀酰亚胺）是常用的伯胺基反应基团。它能够与伯胺基反应，形成稳定的酰胺键，有助于目标分子的有效标记^[5]。Azidobutyric acid NHS ester的化学式为C₈H₁₀N₄O₄，分子量为226.19。本产品仅供研究用途，不适用于食品、药品、医疗器械或化妆品。