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Azithromycin-d3 Sale

(Synonyms: 阿奇霉素 13C-d3) 目录号 : GC46903

An internal standard for the quantification of azithromycin

Azithromycin-d3 Chemical Structure

Cas No.:163921-65-1

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1 mg
¥3,742.00
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Sample solution is provided at 25 µL, 10mM.

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产品描述

Azithromycin-d3 is intended for use as an internal standard for the quantification of azithromycin by GC- or LC-MS. Azithromycin is a macrolide antibiotic.1 It is active against S. pneumoniae, S. aureus, N. gonorrhoeae, M. pneumoniae, H. pylori, C. trachomatis, and H. influenzae in vitro (MIC90s = <0.01-2 mg/L). Azithromycin increases survival in mouse models of intraperitoneal S. pyogenes, S. pneumoniae, E. faecalis, or H. influenzae infection (ED50s = 0.78, 8.7, 12.7, and 30.3 mg/kg, respectively).2 It also decreases plasma levels of IL-6, TNF-α, and IL-1β and increases survival in mouse model of LPS-induced sepsis when administered at a dose of 100 mg/kg.3 Formulations containing azithromycin have been used in the treatment of a variety of bacterial infections.

1.Kanatani, M.S., and Guglielmo, B.J.The new macrolides. Azithromycin and clarithromycinWestern J. Med.160(1)31-37(1994) 2.Girard, D., Finegan, S.M., Dunne, M.W., et al.Enhanced efficacy of single-dose versus multi-dose azithromycin regimens in preclinical infection modelsJ. Antimicrob. Chemother.56(2)365-371(2005) 3.Patel, A., Joseph, J., Periasamy, H., et al.Azithromycin in combination with ceftriaxone reduces systemic inflammation and provides survival benefit in a murine model of polymicrobial sepsisAntimicrob. Agents Chemother.62(9)e00752-00718(2018)

Chemical Properties

Cas No. 163921-65-1 SDF
别名 阿奇霉素 13C-d3
Canonical SMILES C[C@@H]([C@@H]([C@H](C(O[C@H](CC)[C@@](C)(O)[C@H](O)[C@H]1C)=O)C)O[C@@](O[C@@H](C)[C@@H]2O)([H])C[C@@]2(C)OC)[C@H]([C@](O)(C[C@H](CN1C([2H])([2H])[2H])C)C)O[C@@](O[C@H](C)C[C@@H]3N(C)C)([H])[C@@H]3O
分子式 C38H69D3N2O12 分子量 752
溶解度 DMSO: 100 mg/ml,Ethanol: 100 mg/ml 储存条件 Store at -20°C
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储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
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1 mM 1.3298 mL 6.6489 mL 13.2979 mL
5 mM 0.266 mL 1.3298 mL 2.6596 mL
10 mM 0.133 mL 0.6649 mL 1.3298 mL
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Research Update

A LC-MS/MS validated method for determination of azithromycin in human tears and its application to an ocular pharmacokinetic study

Pharmazie 2020 Oct 1;75(10):478-482.PMID:33305720DOI:10.1691/ph.2020.0538

A rapid and sensitive method for the quantitative analysis of azithromycin in human tears by LC-MS/MS was developed and validated. Following extraction from collected Schirmer tear strips by methanol-water (4:1, v/v), the analyte and IS (Azithromycin-d3) were separated on a Waters Atlantis™ dC18 column (2.1 mm × 30 mm, 3 μm) by gradient elution with 0.1% (v/v) formic acid in methanol-water (1:9) and methanol-acetonitrile (9:1) as the mobile phase. Electrospray ionization in positive ion mode and MRM were used to monitor the ion transitions at m/z 749.6 → 591.6 (azithromycin) and 752.4 → 594.4 (Azithromycin-d3). The results indicated that the method had excellent sensitivity and specificity. The analyte appeared to have good linearity in the range of 5-1000 ng/ mL. Both the intra-batch and inter-batch precisions (in terms of RSD) were <10%, and the accuracies (in terms of RE) were within ±15%. The lower limit of quantification, matrix effect, extraction recovery, stability and dilution integrity were also evaluated and satisfied the validation criteria. Artificial tears served as the surrogate matrix, and no matrix difference was found when compared with that of real human tears. Finally, this method was successfully applied in an ocular pharmacokinetic study in healthy volunteers following instillation of azithromycin eyedrops.