Azoxymethane
(Synonyms: 偶氮甲烷; AOM) 目录号 : GC19445偶氮甲烷作为一种结肠致癌物,可导致DNA加合物的形成。
Cas No.:25843-45-2
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Azoxymethane, as a colon carcinogen, can lead to the formation of DNA adducts[1]. Azoxymethane (AOM) causes O(6)-methylguanine adduct formation which leads to G-->A transitions[2].
In vivo, 7-week-old conventional male C57BL/6 mice were injected intraperitoneally with 6 doses of 10 mg/kg AOM, feces from obese individuals promote colorectal cancer formation in AOM-treated CRC mouse model[3]. SWR/J, A/J mice was treated with 10 mg/kg AOM i.p. once a week for 8 weeks developed tumors, with a distribution that was limited to the distal colon[4]. In wild-type (WT) mice, AOM treatment (10 mg/kg) induced a 2.5-fold increase in intestinal crypt stem cell survival. WT mice receiving AOM exhibited increased intestinal apoptosis and a simultaneous reduction in crypt mitotic figures within 8 h of injection[5].
[1] Chen W, et al. Effect of tea on the formation of DNA adducts by azoxymethane. Xenobiotica. 1998 Feb;28(2):213-7.
[2] Wali RK, et al. Inhibition of O(6)-methylguanine-DNA methyltransferase increases azoxymethane-induced colonic tumors in rats. Carcinogenesis. 1999 Dec;20(12):2355-60.
[3] Kang X, et al. Altered gut microbiota of obesity subjects promotes colorectal carcinogenesis in mice. EBioMedicine. 2023 Jul;93:104670.
[4] Papanikolaou A, et al. Azoxymethane-induced colon tumors and aberrant crypt foci in mice of different genetic susceptibility. Cancer Lett. 1998 Aug 14;130(1-2):29-34.
[5] Riehl TE, et al. Azoxymethane protects intestinal stem cells and reduces crypt epithelial mitosis through a COX-1-dependent mechanism. Am J Physiol Gastrointest Liver Physiol. 2006 Dec;291(6):G1062-70.
References:
偶氮甲烷作为一种结肠致癌物,可导致DNA加合物的形成[1]。偶氮甲烷(AOM)导致O(6)-甲基鸟嘌呤加合物的形成,从而导致G->A跃迁[2]。
在体内,7周龄的常规雄性C57BL/6小鼠腹膜内注射6剂量的10 mg/kg AOM,来自肥胖个体的粪便促进AOM治疗的CRC小鼠模型中结直肠癌癌症的形成[3]。SWR/J,A/J小鼠用10 mg/kg AOM腹腔注射治疗,每周一次,持续8周,出现肿瘤,其分布仅限于远端结肠[4]。在野生型(WT)小鼠中,AOM处理(10 mg/kg)诱导肠隐窝干细胞存活率增加2.5倍。接受AOM的WT小鼠在注射后8小时内表现出肠细胞凋亡增加和隐窝有丝分裂图同时减少[5]。
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 13.4989 mL | 67.4946 mL | 134.9892 mL |
| 5 mM | 2.6998 mL | 13.4989 mL | 26.9978 mL |
| 10 mM | 1.3499 mL | 6.7495 mL | 13.4989 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。