β-Glucuronidase from bovine liver
(Synonyms: β-葡糖苷酸酶;β-葡萄糖醛酸甙酶;β-葡萄糖醛酸苷酶) 目录号 : GC19783
β-Glucuronidase from bovine liver是一种重要的溶酶体酶,参与含葡萄糖醛酸的糖胺聚糖的降解。
Cas No.:9001-45-0
Sample solution is provided at 25 µL, 10mM.
β-Glucuronidase from bovine liver is an important lysosomal enzyme involved in the degradation of glucuronic acid-containing glycosaminoglycans[1]. β-Glucuronidase catalyzes the hydrolysis of glucuronic acid derivatives and plays a key physiological role in humans, animals, plants and some bacteria[2]. β-Glucuronidase has been measured as a typical lysosomal enzyme during subcellular fractionation studies[3]. β-Glucuronidase has become a model system for studying genetic regulatory polymorphisms, androgen induction mechanisms, lysosomal enzyme processing and intracellular enzyme localization mechanisms[4]. β-Glucuronidase deficiency causes mucopolysaccharidosis type VII (MPSVII), leading to lysosomal accumulation in the brain[5]. β-Glucuronidase has a dissociative effect on bilirubin and can convert conjugated bilirubin into an unconjugated form[6].
Activity: ≥100,000units/g.
Definition of enzyme activity: At 37°C and pH 5.0, a modified Fishman apparatus will release 1.0μg of phenolphthalein from phenolphthalein glucuronide per hour.
References:
[1] Brüsselbach S. Extracellular β-Glucuronidase for Gene-Directed Enzyme-Prodrug Therapy[J]. Suicide Gene Therapy: Methods and Reviews, 2004: 303-330.
[2] Ibrahim N E D, Shao W, Ma K. Properties and Biotechnological Applications of β-Glucuronidases[M]//Biotechnology of Microorganisms. Apple Academic Press, 2019: 147-178.
[3] Fishman W H. β-Glucuronidase[M]//Methods of enzymatic analysis. Academic Press, 1974: 929-943.
[4] Paigen K. Mammalian β-glucuronidase: genetics, molecular biology, and cell biology[J]. Progress in nucleic acid research and molecular biology, 1989, 37: 155-205.
[5] Roy E. Cell disorders in lysosomal storage diseases[D]. Université René Descartes-Paris V, 2012.
[6] Chowdhury N R, Li Y, Chowdhury J R. Disorders of bilirubin metabolism[J]. The liver: biology and pathobiology, 2020: 229-244.
β-Glucuronidase from bovine liver是一种重要的溶酶体酶,参与含葡萄糖醛酸的糖胺聚糖的降解[1]。β-Glucuronidase能够催化葡萄糖醛酸衍生物的水解,该酶在人类、动物、植物和一些细菌中发挥关键生理作用[2]。在亚细胞组分化研究期间,β-Glucuronidase已被作为典型的溶酶体酶测量[3]。β-Glucuronidase已成为研究遗传调控多态性、雄激素诱导机制、溶酶体酶加工和细胞内酶定位机制的模型系统[4]。β-Glucuronidase缺乏会引起粘多糖沉积症VII型(MPSVII),导致大脑中的溶酶体蓄积[5]。β-Glucuronidase对胆红素具有解离作用,能够将结合胆红素转化为非结合形式[6]。
酶活性:≥100,000units/g。
酶活性定义:在37℃、pH 5.0条件下,一个改良Fishman装置每小时将从酚酞葡糖苷酸中释放1.0μg酚酞。
Cas No. | 9001-45-0 | SDF | |
别名 | β-葡糖苷酸酶;β-葡萄糖醛酸甙酶;β-葡萄糖醛酸苷酶 | ||
分子式 | 分子量 | ||
溶解度 | water : 10 mg/mL (Need ultrasonic and warming) | 储存条件 | Store at -20℃, protect from light, stored under nitrogen |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Biological Activity: 1383.764U/mg solid
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet