b NGF Human, HEK
目录号 : GP26007BNGFHuman Recombinant produced inHEK293 is a noncovalently disulfide linked homodimer, glycosylated, polypeptide chain (Ser122-Arg239) containing 2 identical 118 amino acids and having a molecular mass of 26
Sample solution is provided at 25 µL, 10mM.
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BNGFHuman Recombinant produced inHEK293 is a noncovalently disulfide linked homodimer, glycosylated, polypeptide chain (Ser122-Arg239) containing 2 identical 118 amino acids and having a molecular mass of 26.5 kDa.
| Purity | Greater than 97% as obsereved by SDS-PAGE. | Source | HEK293 cells. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | atRoomtemp. |
| Synonyms | Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428. | ||
| Solubility | It is recommended to reconstitute the lyophilized b-NGF in steriledistilled pyrogen free waterat a concentration of 0.25mg/ml. | ||
| Stability | Lyophilized b-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18буC. Upon reconstitution b-NGF should be stored at 4буC between 2-7 days and for future use below -18буC.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Biological Activity | The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line), the ED50 is <0.04-0.4ng/ml. | ||
| Formulation | The b-NGF was lyophilized from 1mg/ml in20mM PB and 0.25M NaClpH-7.5. | ||
NGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis.
The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line), the ED50 is <0.04-0.4ng/ml.
Lyophilized b-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18буC. Upon reconstitution b-NGF should be stored at 4буC between 2-7 days and for future use below -18буC.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.