BAFF Human, Plant
目录号 : GP20092BAFF (BLyS) Human Recombinant, Plant
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 97.0% as determined by Analysis by SDS-PAGE. | Source | Nicotiana benthamiana plant |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20; TNFSF13B; B-cell Activating Factor. | ||
| Amino Acid Sequence | HHHHHHHHHH AVQGPEETVT QDCLQLIADS ETPTIQKGSY TFVPWLLSFK RGSALEEKEN KILVKETGYF FIYGQVLYTD KTYAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PRENAQISLD GDVTFFGALK LL | ||
| Solubility | It is recommended to reconstitute the lyophilized BAFF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | LyophilizedBAFF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitutionBAFF should be stored at 4°C between 2-7 days and for future use below -18°C . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | ||
| Biological Activity | The activity is determined by dose-dependent stimulation of proliferation B cell from Human PBMC. Cell proliferation was measured by MTT method. *activity results may vary with PBMC donors. ED50 ? 50ng/ml | ||
| Formulation | Lyophilized from 1mg/ml solution in 20 mM PBS buffer pH 7 and 0.2 M NaCl. | ||
BAFF binds to tnfrsf13b/taci and tnfrsf17/bcma. Tnfsf13/april binds to the same 2 receptors, together, they form a 2 ligands -2 receptors pathway involved in the stimulation of b- and t-cell function and the regulation of humoral immunity.A third b-cell specific baff-receptor (baffr/br3) promotes the survival of mature b-cells and the b-cell response.B Lymphocyte Stimulator functions as a potent B-cell growth factor in costimulation assays. Administration of BAFF Human recombinant to mice disrupts splenic B-cell and T-cell zones and results in elevated levels of serum immunoglobulin.
The activity is determined by dose-dependent stimulation of proliferation B cell from Human PBMC. Cell proliferation was measured by MTT method. *activity results may vary with PBMC donors. ED50 ? 50ng/ml
LyophilizedBAFF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitutionBAFF should be stored at 4°C between 2-7 days and for future use below -18°C . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.