BAM-22P (8-22) (human, mouse, rat, bovine) (trifluoroacetate salt)
(Synonyms: BAM 8-22, Bovine Adrenal Medulla 8-22) 目录号 : GC52409A neuropeptide and MRGPRX1 agonist
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
BAM-22P (8-22) is an endogenous neuropeptide derived from proenkephalin A and an agonist of MAS-related G protein-coupled receptor family member X1 (MRGPRX1), previously known as sensory neuron-specific G protein-coupled receptor 4 (SNSR4).1 It is involved in prurition and nociception. BAM-22P (8-22) induces calcium mobilization in HEK293S cells expressing human MRGPRX1 or SNSR3 (EC50s = 14 and 28 nM, respectively) and inhibits voltage-induced calcium currents in isolated rat neurons expressing human MRGPRX1 (EC50 = 0.6 µM).2 Intrathecal administration of BAM-22P (8-22) (30 nmol/animal) decreases the tail-flick latency in rats stimulated with the Gαi inhibitor pertussin toxin and increases the tail-flick latency in rats stimulated with the phospholipase C (PLC) inhibitor U-73122, indicating changes in pain sensitivity.3 BAM-22P (8-22) (100 µg/animal) increases scratching behavior in wild-type mice and enhances bile-duct ligation-induced scratching behavior in a mouse model of cholestasis.4
1.Lembo, P.M.C., Grazzini, E., Groblewski, T., et al.Proenkephalin A gene products activate a new family of sensory neuron-specific GPCRsNat. Neurosci.5(3)201-209(2002) 2.Chen, H., and Ikeda, S.R.Modulation of ion channels and synaptic transmission by a human sensory neuron-specific G-protein-coupled receptor, SNSR4/mrgX1, heterologously expressed in cultured rat neuronsJ. Neurosci.24(21)5044-5053(2004) 3.Chen, T., Wang, D., and Hong, Y.Dual modulation effects of Mas-related gene (Mrg) receptors on pain sensitivity in ratsNeurosci. Lett.514(1)82-85(2012) 4.Sanjel, B., Maeng, H.-J., and Shim, W.-S.BAM8-22 and its receptor MRGPRX1 may attribute to cholestatic pruritusSci. Rep.9(1)10888(2019)
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.5073 mL | 2.5365 mL | 5.0731 mL |
| 5 mM | 0.1015 mL | 0.5073 mL | 1.0146 mL |
| 10 mM | 0.0507 mL | 0.2537 mL | 0.5073 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。