BCECF (2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein)
(Synonyms: 2,7-双(2-羧乙基)-5(6)-羧基荧光素,2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein) 目录号 : GC30270
A fluorescent pH probe
Cas No.:85138-49-4
Sample solution is provided at 25 µL, 10mM.
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BCECF is a fluorescent probe commonly used to measure pH.1 It is a dual-excitation ratiometric pH indicator with a pKa of ~6.98. Measurements of pH are made by determining the ratio of emission intensity, detected at 535 nm, when excited at 490 nm versus the emission intensity when excited at 440 nm.1 BCECF is membrane impermeable, whereas the acetoxymethyl ester, BCEBF-AM is membrane permeant.
1.O'Connor, N., and Silver, R.B.Ratio imaging: Practical considerations for measuring intracellular Ca2+ and pH in living cellsMethods Cell Biol.81415-433(2007)
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Kinase experiment: |
The cells are palced in a petri dish and BCECF present in trichomonads is fixed for electron microsopy. Controns without BCECF loading, without DAB treatment, and without UV illumination are run in parallel. Trichomonads remaine viable during the procedure, except that without BCECF they are dead and disrupted by 4 h. Then the cell are lysed. After lysis, BCECF is added to the lysate to 20 μM, and the lysate is incubated,followed by 3 washes in lysis buffer to remove free dye and resuspension of the pellet in the original volume[1]. |
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References: [1]. Scott DA, et al. Analysis of the uptake of the fluorescent marker 2',7'-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF) by hydrogenosomes in Trichomonas vaginalis. Eur J Cell Biol. 1998 Jun;76(2):139-45. |
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| Cas No. | 85138-49-4 | SDF | |
| 别名 | 2,7-双(2-羧乙基)-5(6)-羧基荧光素,2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein | ||
| Canonical SMILES | O=C(O)CCC1=CC(C23OC(C4=C2C=CC(C(O)=O)=C4)=O)=C(OC5=C3C=C(CCC(O)=O)C(O)=C5)C=C1O.O=C(O)CCC6=CC(C78OC(C9=C7C=C(C(O)=O)C=C9)=O)=C(OC%10=C8C=C(CCC(O)=O)C(O)=C%10)C=C6O | ||
| 分子式 | C27H20O11 | 分子量 | 520.44 |
| 溶解度 | DMSO : 62.5 mg/mL (120.09 mM);Water : < 0.1 mg/mL (insoluble) | 储存条件 | Store at -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.9215 mL | 9.6073 mL | 19.2145 mL |
| 5 mM | 0.3843 mL | 1.9215 mL | 3.8429 mL |
| 10 mM | 0.1921 mL | 0.9607 mL | 1.9215 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >90.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet