BDE No 99
(Synonyms: 2,2',4,4',5-五溴联苯醚, 2,2′,4,4′,5-PentaBDE) 目录号 : GC18657
BDE No 99 是在人体体液和脂肪饮食中检测到的主要多溴联苯醚 (PBDE) 同系物。
Cas No.:60348-60-9
Sample solution is provided at 25 µL, 10mM.
BDE No 99 is the predominant Polybrominated diphenyl ethers(PBDE) congeners detected in human fluid and fatty diet. PBDEs are persistent and bio-accumulative in the environment.[1].
BDE No 99 reduced human and murine oligodendrocyte lineage (O4+) cell formation in a concentration-dependent manner (IC50 1.9?μM and 13.6?μM, respectively and IC20 0.9?μM and 6.9?μM, respectively) with human NPCs being 7-times more sensitive towards BDE No 99 exposure than their murine counterparts. BDE No 99 reduced generation of human and mouse O4+ cells, but there is no indication for BDE No 99 interfering with cellular TH signaling during O4+ cell formation. BDE No 99 reduced hMBP expression due to oligodendrocyte reduction, but concentrations that did not affect the number of mouse O4+ cells inhibited TH-induced mMog transcription by a yet unknown mechanism[2]
BDE No 99 increased unconjugated bile acids(BAs) in the serum, liver, small large intestinal contents(SIC) and large intestinal contents(LIC) of conventional mice. BDE No 99 profoundly decreased the alpha diversity of gut microbiome and differentially regulated 45 bacterial species. BDE No 99 downregulated enzymes and transporters involved in BA metabolism, in a gut microbiome-dependent manner[3]
References:
[1]. Frederiksen M, Vorkamp K, et al. Human internal and external exposure to PBDEs--a review of levels and sources. Int J Hyg Environ Health. 2009 Mar;212(2):109-34.
[2]. Dach K, Bendt F, et al. BDE-99 impairs differentiation of human and mouse NPCs into the oligodendroglial lineage by species-specific modes of action. Sci Rep. 2017 Mar 20;7:44861.
[3]. Li CY, Dempsey JL, et al. PBDEs Altered Gut Microbiome and Bile Acid Homeostasis in Male C57BL/6 Mice. Drug Metab Dispos. 2018 Aug;46(8):1226-1240.
BDE No 99 是在人体体液和脂肪饮食中检测到的主要多溴联苯醚 (PBDE) 同系物。 PBDEs 在环境中具有持久性和生物蓄积性。[1]。
BDE No 99 以浓度依赖性方式减少人和鼠少突胶质细胞谱系 (O4+) 细胞形成（IC50 分别为 1.9μM 和 13.6μM，IC20 分别为 0.9μM 和 6.9μM），人类 NPC 的数量是其 7 倍对 BDE No 99 的暴露比他们的小鼠对应物更敏感。 BDE No 99 减少了人和小鼠 O4+ 细胞的生成，但没有迹象表明 BDE No 99 在 O4+ 细胞形成过程中干扰细胞 TH 信号传导。 BDE No 99 由于少突胶质细胞减少而降低了 hMBP 表达，但不影响小鼠 O4+ 细胞数量的浓度通过未知机制抑制了 TH 诱导的 mMog 转录[2]
BDE No 99 增加了常规小鼠血清、肝脏、小肠内容物 (SIC) 和大肠内容物 (LIC) 中的未结合胆汁酸 (BA)。 BDE No 99 显着降低了肠道微生物组的 alpha 多样性，并对 45 种细菌进行了差异调节。 BDE No 99 以肠道微生物组依赖性方式下调参与 BA 代谢的酶和转运体[3]
| Cell experiment [1]: | |
|
Cell lines |
Human and mouse neural progenitor cells |
|
Preparation Method |
Human mouse neural progenitor cells (NPCs) were differentiated with DMSO or increasing concentrations of BDE No 99 for 5 days. oligodendrocyte lineage cells were immunocytochemically stained with an antibody against O4 and nuclei were counterstained with Hoechst 33258. Viability was measured with the Alamar-Blue assay two hours prior fixation. |
|
Reaction Conditions |
0.01 - 20µM BDE No 99 for 5 days. |
|
Applications |
BDE No 99 reduces NPC differentiation to the oligodendrocyte (O4+ cells) lineage. BDE No 99 reduced human and murine O4+ cell formation in a concentration-dependent manner with human NPCs being 7-times more sensitive towards BDE No 99 exposure than their murine counterparts. |
| Animal experiment [2]: | |
|
Animal models |
C57BL/6J conventional(CV) mice, C57BL/6J germ-free (GN) mice |
|
Preparation Method |
CV and GN mice were randomly allocated for the treatment of vehicle (corn oil, 10 ml/kg), or BDE No 99 via oral gavage once daily for 4 days. Livers were collected 24 hours after the last dosing on the fifth day and immediately frozen in liquid nitrogen. The mRNAs of phase-I drug-metabolizing enzymes Cyp1a2 , Cyp2b10, and Cyp3a11 were quantified in livers of CV mice using RT-qPCR. |
|
Dosage form |
10, 100 μmol/kg BDE No 99, oral gavage |
|
Applications |
Many Cyp2 family members (such as Cyp2a5, Cyp2b10, Cyp2c37, Cyp2c50, Cyp2c54, and Cyp2c55) are upregulated by BDE No 99 in livers of both CV and GF mice, with a much greater fold change observed in GF conditions. |
|
References: [1]. Dach K, Bendt F, et al. BDE-99 impairs differentiation of human and mouse NPCs into the oligodendroglial lineage by species-specific modes of action. Sci Rep. 2017 Mar 20;7:44861. [2]. Li CY, Lee S, et al. Novel Interactions between Gut Microbiome and Host Drug-Processing Genes Modify the Hepatic Metabolism of the Environmental Chemicals Polybrominated Diphenyl Ethers. Drug Metab Dispos. 2017 Nov;45(11):1197-1214. |
|
| Cas No. | 60348-60-9 | SDF | |
| 别名 | 2,2',4,4',5-五溴联苯醚, 2,2′,4,4′,5-PentaBDE | ||
| 化学名 | 1,2,4-Tribromo-5-(2,4-dibromophenoxy)benzene | ||
| Canonical SMILES | BrC1=C(OC2=CC(Br)=C(Br)C=C2Br)C=CC(Br)=C1 | ||
| 分子式 | C12H5Br5O | 分子量 | 564.69 |
| 溶解度 | 50 μg/mL in isooctane | 储存条件 | Store at 2-8°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.7709 mL | 8.8544 mL | 17.7088 mL |
| 5 mM | 0.3542 mL | 1.7709 mL | 3.5418 mL |
| 10 mM | 0.1771 mL | 0.8854 mL | 1.7709 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
