Benzenebutyric acid (4-Phenylbutyric acid)
(Synonyms: 4-苯基丁酸; 4-PBA; Benzenebutyric acid) 目录号 : GC30763
4-Phenylbutyric acid (4-PBA, Benzenebutyric acid) is a histone deacetylase (HDAC) inhibitor and a key epigenetic inducer of anti-HCV hepatic hepcidin. 4-Phenylbutyric acid inhibits LPS-induced inflammation through regulating endoplasmic-reticulum (ER) stress and autophagy in acute lung injury models.
Cas No.:1821-12-1
Sample solution is provided at 25 µL, 10mM.
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4-Phenylbutyric acid (4-PBA, Benzenebutyric acid) is a histone deacetylase (HDAC) inhibitor and a key epigenetic inducer of anti-HCV hepatic hepcidin. 4-Phenylbutyric acid inhibits LPS-induced inflammation through regulating endoplasmic-reticulum (ER) stress and autophagy in acute lung injury models.
4-PBA inhibits the ER stress induced by SiNPs in RAW264.7 cells, as evidenced by the expansion and degranulation of ER, as well as greatly up-regulated Bip and CHOP expressions.[3]
4-PBA attenuates LPS-induced bone loss in lipopolysaccharide (LPS)-treated mice, by increasing area of TRAP-positive osteoclasts (OCs) and serum level of collagen type I fragments.[4]
4-苯基丁酸（4-PBA，苯丁酸）是组蛋白去乙酰化酶（HDAC）抑制剂，也是促进抗HCV肝细胞铁调蛋白表达的关键表观遗传诱导剂。4-苯基丁酸通过调节内质网应激和自噬，在急性肺损伤模型中抑制LPS诱导的炎症。
4-苯基丁酸抑制SiNPs诱导的RAW264.7细胞内质网应激，表现为内质网的扩张和脱颗粒化，以及Bip和CHOP表达的显著上调。[3]
4-苯基丁酸通过增加TRAP阳性成骨细胞（OCs）面积和血清胶原I片段水平，减轻了LPS处理小鼠骨丢失的程度。[4]
[1] Kiyoon Kim, et al. Int J Mol Sci. 2020 Aug 1;21(15):5516. [2] Meichun Zeng, et al. Toxicol Lett. 2017 Apr 5;271:26-37. [3] Ma R, et al. Part Fibre Toxicol. 2020;17(1):50.
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Cell experiment: |
Briefly, viable cells, as judged by trypan blue dye exclusion, are seeded at a density of 4×104 cells/mL in 60-mm dishes in RPMI 1640 with 10% fetal bovine serum and 0.35% agarose on a base layer of 0.7% agarose. DMSO, TSA, or PB is added to both bottom and top agarose layers. Assays are performed in triplicate on at least three separate occasions, and colonies are counted at 10-14 days[1]. |
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Animal experiment: |
Mice[3]Female 10-week-old C57BL/6J mice are housed in the pathogen-free animal facility of IRC. Animals are randomized into the following 4 groups: vehicle control (n=5), vehicle+Benzenebutyric acid (n=6), LPS (n=6), and LPS+Benzenebutyric acid (n=6). Mice are treated with LPS in 200 μL phosphate-buffered saline (PBS) once a week (5 mg/kg, i.p.) for 3 weeks. Benzenebutyric acid solution is prepared by titrating equimolecular amounts of Benzenebutyric acid and sodium hydroxide to reach pH 7.4; mice are injected daily intraperitoneally in 200 μL PBS (or with PBS as a vehicle) at a dose of 240 mg/kg for 3 weeks. Mice are sacrificed by CO2 asphyxiation. To determine the bone mineral density (BMD) and microarchitecture of the long bone, the right femur is scanned. Scans are performed with an effective detector pixel size of 6.9 μm and a threshold of 77-255 mg/cc. Trabecular bone is analyzed in a region 1.6 mm in length and located 0.1 mm below the distal femur growth plate[3]. |
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References: [1]. Chang TH, et al. Enhanced growth inhibition by combination differentiation therapy with ligands of peroxisome proliferator-activated receptor-gamma and inhibitors of histone deacetylase in adenocarcinoma of the lung. Clin Cancer Res. 2002 Apr;8(4):1206-12. |
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| Cas No. | 1821-12-1 | SDF | |
| 别名 | 4-苯基丁酸; 4-PBA; Benzenebutyric acid | ||
| Canonical SMILES | O=C(O)CCCC1=CC=CC=C1 | ||
| 分子式 | C10H12O2 | 分子量 | 164.2 |
| 溶解度 | DMSO : ≥ 125 mg/mL (761.27 mM);Water : 2 mg/mL (12.18 mM) | 储存条件 | Store at RT |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.0901 mL | 30.4507 mL | 60.9013 mL |
| 5 mM | 1.218 mL | 6.0901 mL | 12.1803 mL |
| 10 mM | 0.609 mL | 3.0451 mL | 6.0901 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet