Benzovindiflupyr
目录号 : GC49318
A fungicide
Cas No.:1072957-71-1
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >90.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Benzovindiflupyr is a fungicide.1 It inhibits mitochondrial complex II, also known as succinate dehydrogenase (SDH; IC50 = 5.2 nM), and mycelial growth of S. sclerotiorum (EC50 = 0.011 µg/ml). Benzovindiflupyr (60 µg/ml) completely protects eggplant leaves from S. sclerotiorum infection. Formulations containing benzovindiflupyr have been used to control various fungal diseases in agriculture.
1.Gao, Y., He, L., Zhu, J., et al.The relationship between features enabling SDHI fungicide binding to the Sc-Sdh complex and its inhibitory activity against Sclerotinia sclerotiorumPest Manag. Sci.76(8)2799-2808(2020)
| Cas No. | 1072957-71-1 | SDF | |
| Canonical SMILES | O=C(C1=CN(C)N=C1C(F)F)NC2=CC=CC3=C2C4/C(C3CC4)=C(Cl)\Cl | ||
| 分子式 | C18H15Cl2F2N3O | 分子量 | 398.2 |
| 溶解度 | Acetonitrile: soluble | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.5113 mL | 12.5565 mL | 25.113 mL |
| 5 mM | 0.5023 mL | 2.5113 mL | 5.0226 mL |
| 10 mM | 0.2511 mL | 1.2557 mL | 2.5113 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Toxic effects of Benzovindiflupyr, a new SDHI-type fungicide on earthworms (Eisenia fetida)
Environ Sci Pollut Res Int 2021 Nov;28(44):62782-62795.PMID:34215985DOI:10.1007/s11356-021-15207-4.
Benzovindiflupyr has received increasing attention as a new novel succinate dehydrogenase inhibitor (SDHI)-type fungicide. Nonetheless, its traces remaining in soil potentially trigger an ecotoxicological threat to soil organisms including earthworms. This paper evaluates the eco-toxicity of different Benzovindiflupyr doses (0.1, 1, 5, and 10 mg kg-1) on earthworms (Eisenia fetida) after long-term exposure. Consequently, Benzovindiflupyr at higher doses significantly inhibited the activities of respiratory chain complex II and succinate dehydrogenase (SDH) in E. fetida. Besides, the reactive oxygen species (ROS) and lipid peroxidation (LPO) were significantly induced in earthworms when treated with this fungicide. After Benzovindiflupyr exposure, activities of antioxidant enzymes including catalase, peroxidase, and superoxide dismutase were activated. However, glutathione S-transferase activity in E. fetida was initially induced then inhibited in earthworms after treatment. Furthermore, Benzovindiflupyr exposure induced the protein carbonylation (PCO) level in cells indicating oxidative damage to the cellular protein. Due to the destruction of the normal function in the coelomocytes, the phagocytic activity was initially activated, then inhibited when earthworms were treated at 5 and 10 mg kg-1 concentrations. Additionally, DNA damage was induced (larger olive tail moment (OTM) values) with the increase of Benzovindiflupyr doses and exposure time. The weight was significantly decreased after Benzovindiflupyr exposure on days 21 and 28. Benzovindiflupyr at higher doses significantly decreased the reproduction (number of cocoons and juveniles) of E. fetida. These findings reveal that Benzovindiflupyr potentially induces a potential toxicological risk to earthworms when applied in the mentioned above dosages.
The Bioactivity and Efficacy of Benzovindiflupyr Against Corynespora cassiicola, the Causal Agent of Cucumber Corynespora Leaf Spot
Plant Dis 2021 Oct;105(10):3201-3207.PMID:33560881DOI:10.1094/PDIS-11-20-2334-RE.
Corynespora cassiicola, which causes Corynespora leaf spot, results in considerable yield loss of cucumber grown in greenhouses. Frequent reports of reduced efficacy and control failure of fungicides warrant new, efficient alternative chemistries. In this study, the sensitivity of C. cassiicola to Benzovindiflupyr was evaluated using a collection of 81 isolates collected from Shandong, China. The mean EC50 values for mycelial growth, spore germination, and germ tube elongation of C. cassiicola were 0.69 ± 0.44, 0.12 ± 0.063, and 0.13 ± 0.076 µg ml-1, respectively. Benzovindiflupyr treatment led to a reduced respiration rate and ATP production of C. cassiicola and decreased spore pathogenicity by 21.9% on average. Additionally, detached cucumber leaves sprayed with fungicides before or after inoculation were used to assess the efficacy of Benzovindiflupyr against C. cassiicola. Benzovindiflupyr (150 µg ml-1) exhibited preventive and curative efficacies of 86.9 and 77.1%, respectively. Benzovindiflupyr at 150 g a.i. ha-1 provided over 70% efficacy in field trials performed in 2018 and 2019, which was significantly higher than that of the reference fungicides fluopyram and fluxapyroxad at the same dose. Furthermore, the yield of commercial cucumber increased as disease incidence decreased. Our findings pave the way for the introduction of Benzovindiflupyr in the integrated management of Corynespora leaf spot.
Foliar application of Benzovindiflupyr shows non-fungicidal effects in wheat plants
Pest Manag Sci 2018 Mar;74(3):665-671.PMID:28984411DOI:10.1002/ps.4754.
Background: The fungicide Benzovindiflupyr belongs to the class of succinate dehydrogenase inhibitors (SDHIs). Certain SDHIs have shown plant physiological effects, so-called secondary effects, that appeared to be related to the plant water status. Therefore, the effect of Benzovindiflupyr on transpiration of leaves and whole wheat plants was studied under controlled conditions. Furthermore, wheat yield trials under controlled and natural drought stress in the field were conducted. Results: Transpiration of detached wheat leaves was reduced by Benzovindiflupyr in a dose-dependent manner. Similarly, whole-plant transpiration decreased for several days following application of this fungicide. In 16 field trials under drought stress conditions that were classified as disease-free, treatment of wheat plants at the flag leaf stage or at heading with Benzovindiflupyr showed a grain yield increase (+5.2%; P ≤ 0.01) that was partially attributed to an increased thousand-grain weight. Conclusions: Water saving during pre-anthesis as a result of Benzovindiflupyr application may be associated with better seed setting and filling under dry field conditions in wheat. The results of this research provide new insights into secondary effects of SDHIs that lead directly to yield improvements. © 2017 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Risk Evaluation of Benzovindiflupyr Resistance of Verticillium dahliae Population in Maine
Plant Dis 2023 Mar;107(3):834-839.PMID:35997670DOI:10.1094/PDIS-06-22-1384-RE.
Verticillium dahliae causes Verticillium wilt, resulting in significant losses to potato production. Benzovindiflupyr, a succinate dehydrogenase inhibitor, effectively controls V. dahliae. However, frequent applications of the chemical may expedite the development of fungicide resistance in the pathogen population. To evaluate the risk of Benzovindiflupyr resistance, 38 V. dahliae strains were obtained from diseased potatoes in Maine. The sensitivity of the field population was determined based on effective concentration for 50% inhibition (EC50), which ranged from 0.07 to 11.28 μg ml-1 with a median of 1.08. Segregated clusters of EC50 values indicated that Maine V. dahliae populations have developed Benzovindiflupyr resistance. By exposing conidia of V. dahliae to a high concentration of Benzovindiflupyr, 18 benzovindiflupyr-resistant mutants were obtained. To examine their fitness, the mutants were continuously subculture-transferred for up to 10 generations. Mycelial growth, conidial production, competitiveness, pathogenicity, and cross resistance of the 10th generation mutants were examined. Results showed that 50% of the resistant mutants retained an adaptive level in mycelial growth, and 60% maintained conidial production similar to their parents. Pathogenicity did not change for any of the mutants. No cross resistance was detected between Benzovindiflupyr and either azoxystrobin, boscalid, fluopyram, or pyrimethanil. Thus, the resistance risk in V. dahliae to Benzovindiflupyr should be considered in Maine potato production.
Bioactivity and control efficacy of Benzovindiflupyr against Athelia rolfsii in China
Plant Dis 2023 Jan 23.PMID:36691274DOI:10.1094/PDIS-10-22-2463-RE.
Athelia rolfsii is a devastating soil-borne pathogen that causes stem rot of peanut and severely constrains the peanut production. The new generation of succinate dehydrogenase inhibitor (SDHI) fungicide Benzovindiflupyr has been registered in United States and Brazil for managing multiple plant diseases. However, it is not registered in China to control peanut stem rot. In this study, 246 isolates obtained from major peanut production areas in Shandong, Henan and Hebei Province of China were used to determine the baseline sensitivity of A. rolfsii to Benzovindiflupyr. The frequency of EC50 values of Benzovindiflupyr was unimodally distributed with an average EC50 of 0.12 mg/L and a range of 0.01-0.57 mg/L. Benzovindiflupyr can also strongly inhibit the germination of sclerotia, with an average EC50 of 2.38 mg/L (N= 23). In addition, Benzovindiflupyr exhibited great in vivo efficacy against A. rolfsii, that the protective or curative efficacy (89.87%, 20.39%) of Benzovindiflupyr at a concentration of 50 mg/L was equivalent to that of the control fungicide thifluzamide at 100 mg/L (86.39%, 16.21%). At the same concentration (e.g., 100 mg/L), the protective efficacy (93.99%) of Benzovindiflupyr was more than twice as high as the curative efficacy (45.07%). A positive correlation was existed between Benzovindiflupyr and isopyrazam or mefentrifluconazole, which was possibly resulted from similar chemical structures or the damage of cell membrane. Our findings provided valuable information for the application of Benzovindiflupyr, and the established baseline sensitivity could facilitate the monitoring and assessment of Benzovindiflupyr resistance risk.