Beta-Sitosterol
(Synonyms: β-谷甾醇 (purity>98%); β-Sitosterol (purity>98%); 22,23-Dihydrostigmasterol (purity>98%))) 目录号 : GC20142Beta-Sitosterol是一种具有多种生物活性的植物甾醇，具有抗炎、抗肿瘤、退热和免疫调节等活性。
Cas No.:83-46-5
Sample solution is provided at 25 µL, 10mM.
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Beta-Sitosterol is a plant sterol with multiple biological activities, including anti-inflammatory, anti-tumor, antipyretic and immunomodulatory activities[1, 2]. Beta-Sitosterol can inhibit the migration of vascular smooth muscle cells through the PPARG/AMPK/mTOR pathway[3].
In vitro, Beta-Sitosterol (10-160μM) treatment of MH7A cells for 12-48h inhibited cell proliferation in a concentration- and time-dependent manner, significantly reduced the expression of MMP9, HSP90AA1, SRC, EGFR and ALB genes, and significantly increased the expression of CASP3 protein[4]. Beta-Sitosterol (0.2-1mM) treatment of human liver cancer HepG2 cells for 24h induced cytotoxicity in a dose-dependent manner and induced intracellular ROS accumulation[5].
In vivo, Beta-Sitosterol (50, 100, 200mg/kg) was intraperitoneally injected into rats with paw edema and inhibited edema in a dose-dependent manner, with the inhibitory effect reaching 50-70%[6].
References:
[1] Nandi S, Nag A, Khatua S, et al. Anticancer activity and other biomedical properties of β‐sitosterol: Bridging phytochemistry and current pharmacological evidence for future translational approaches[J]. Phytotherapy Research, 2024, 38(2): 592-619.
[2] Fraile L, Crisci E, Córdoba L, et al. Immunomodulatory properties of beta-sitosterol in pig immune responses[J]. International Immunopharmacology, 2012, 13(3): 316-321.
[3] He S, He S, Chen Y, et al. Beta-sitosterol modulates the migration of vascular smooth muscle cells via the PPARG/AMPK/mTOR pathway[J]. Pharmacology, 2022, 107(9-10): 495-509.
[4] Wang X, Mao J. Systematic pharmacology-based strategy to investigate the mechanism of beta-sitosterol for the treatment of rheumarthritis[J]. Frontiers in Genetics, 2024, 15: 1507606.
[5] Ditty M J, Ezhilarasan D. β-sitosterol induces reactive oxygen species-mediated apoptosis in human hepatocellular carcinoma cell line[J]. Avicenna Journal of Phytomedicine, 2021, 11(6): 541.
[6] Paniagua-Pérez R, Flores-Mondragón G, Reyes-Legorreta C, et al. Evaluation of the anti-inflammatory capacity of beta-sitosterol in rodent assays[J]. African Journal of Traditional, Complementary and Alternative Medicines, 2017, 14(1): 123-130.
Beta-Sitosterol是一种具有多种生物活性的植物甾醇，具有抗炎、抗肿瘤、退热和免疫调节等活性[1, 2]。Beta-Sitosterol能够通过PPARG/AMPK/mTOR途径抑制血管平滑肌细胞的迁移[3]。
在体外，Beta-Sitosterol（10-160μM）处理MH7A细胞12-48h，以浓度和时间依赖性方式抑制了细胞增殖，显著降低了MMP9、HSP90AA1、SRC、EGFR和ALB基因的表达，显著增加了CASP3蛋白的表达[4]。Beta-Sitosterol（0.2-1mM）处理人肝癌HepG2细胞24h，以剂量依赖性方式诱导了细胞毒性，诱导了细胞内ROS积累[5]。
在体内，Beta-Sitosterol（50, 100, 200mg/kg）通过腹腔注射治疗爪水肿大鼠，以剂量依赖性方式抑制了水肿，抑制效果达到了50-70%[6]。
| Cell experiment [1]: | |
Cell lines | MH7A cells |
Preparation Method | The test was divided into the control group (DMSO), positive drug group (methotrexate, 80μM), and drug intervention group (Beta-Sitosterol, 10, 20, 40, 80, and 160μM, respectively). After cells were treated with different concentrations of Beta-Sitosterol, the MTT method was used to detect the proliferation of cells at 12, 24, and 48h. |
Reaction Conditions | 10, 20, 40, 80, 160μM; 12, 24, 48h |
Applications | Beta-Sitosterol inhibits the proliferation of MH7A cells in a concentration- and time-dependent manner. |
| Animal experiment [2]: | |
Animal models | Wistar rats |
Preparation Method | Used 30 rats organized in 6 groups with 5 individuals each: a negative control group that was intra-gastrically (IG) administered with mineral oil; three other groups that were IG administered with Beta-Sitosterol (50, 100 and 200mg/kg), previously diluted in mineral oil; and finally, two more groups intraperitoneally (i.p.) administered with known anti-inflammatory drugs, in which one group was injected 200mg/kg of ibuprofen (a non-steroidal anti-inflammatory agent), and the other group was injected with 10mg/kg of prednisone (a steroidal anti-inflammatory agent). All animals were immediately injected with 0.1mL of anti-ovalbumin antibody (diluted 1:3) in the hindquarter of the plantar area of the right leg, and in the contralateral leg; rats were injected with 0.1mL of 0.9% NaCl. In a subsequent step, all animals were intravenously (i.v.) injected with a suspension of egg albumin (25mg/kg). The volume of the swollen leg was determined with a digital pletysmometer 3h after the ovoalbumin injection. The paw edema value was established by determining the difference between the volume obtained in the treated leg and in the control (left leg). For data calculation, the volume of the swollen leg was considered to represent 100% inflammation. |
Dosage form | 50, 100, 200mg/kg; i.p. |
Applications | The three tested doses of Beta-Sitosterol were found to induce a significant, dose dependent inhibition of the edema in comparison with the result found in the control group. The inhibition was 51, 63, and 70% with 50, 100, and 200mg/kg of Beta-Sitosterol, respectively. |
References: | |
| Cas No. | 83-46-5 | SDF | |
| 别名 | β-谷甾醇 (purity>98%); β-Sitosterol (purity>98%); 22,23-Dihydrostigmasterol (purity>98%)) | ||
| 分子式 | C29H50O | 分子量 | 414.71 |
| 溶解度 | Ethanol : 5 mg/mL (12.06 mM; ultrasonic and warming and heat to 60°C) | 储存条件 | -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4113 mL | 12.0566 mL | 24.1132 mL |
| 5 mM | 0.4823 mL | 2.4113 mL | 4.8226 mL |
| 10 mM | 0.2411 mL | 1.2057 mL | 2.4113 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet