Betanin
(Synonyms: 甜菜红) 目录号 : GC31409
Betanin是一种从甜菜中提取的红色糖苷，是一种水溶性含氮色素，可用作食品着色剂。
Cas No.:7659-95-2
Sample solution is provided at 25 µL, 10mM.
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Betanin is a red glycoside extracted from beets. It is a water-soluble nitrogen-containing pigment that can be used as a food colorant[1]. Betanin is unstable and easily degraded under light and high temperature[2]. Betanin has anti-inflammatory and antioxidant activities and also has neuroprotective effects[3].
In vitro, Betanin (10-80µM) treatment of human chronic myeloid leukemia cell line K562 cells for 12-48h dose- and time-dependently inhibited cell viability, induced cell apoptosis, induced the release of cytochrome c from mitochondria to the cytosol, and reduced membrane potential[4]. Betanin (15μM) treatment of THP-1 cells for 24h significantly induced the expression of IL-1β, IL-8, and IL-10 mRNA in cells[5].
In vivo, oral administration of Betanin (100mg/kg) to a neurodegenerative disease model mouse for 2 weeks had a significant anxiolytic effect, prevented hippocampal CA1 degeneration and changes in choline acetyltransferase (ChAT) activity, and improved spatial learning and memory impairment[6]. Oral administration of Betanin (20mg/kg) to a hyperlipidemic diet-induced oxidative stress rat for 20 days reduced liver malondialdehyde content, increased superoxide dismutase, catalase, and glutathione peroxidase activities, and reversed liver tissue damage[7].
References:
[1] Coy-Barrera E. Analysis of betalains (betacyanins and betaxanthins)[M]//Recent advances in natural products analysis. Elsevier, 2020: 593-619.
[2] Azeredo H M C. Betalains: properties, sources, applications, and stability–a review[J]. International journal of food science & technology, 2009, 44(12): 2365-2376.
[3] Thong-Asa W, Puenpha K, Lairaksa T, et al. Neuroprotective effects of betanin in mice with cerebral ischemia-reperfusion injury[J]. Experimental Animals, 2023, 72(3): 336-345.
[4] Sreekanth D, Arunasree M K, Roy K R, et al. Betanin a betacyanin pigment purified from fruits of Opuntia ficus-indica induces apoptosis in human chronic myeloid leukemia Cell line-K562[J]. Phytomedicine, 2007, 14(11): 739-746.
[5] Muramatsu D, Uchiyama H, Higashi H, et al. Effects of heat degradation of betanin in red beetroot (Beta vulgaris L.) on biological activity and antioxidant capacity[J]. Plos one, 2023, 18(5): e0286255.
[6] Thong-Asa W, Prasartsri S, Klomkleaw N, et al. The neuroprotective effect of betanin in trimethyltin-induced neurodegeneration in mice[J]. Metabolic Brain Disease, 2020, 35: 1395-1405.
[7] da Silva D V T, Pereira A D A, Boaventura G T, et al. Short-term betanin intake reduces oxidative stress in wistar rats[J]. Nutrients, 2019, 11(9): 1978.
Betanin是一种从甜菜中提取的红色糖苷，是一种水溶性含氮色素，可用作食品着色剂[1]。Betanin不稳定，在光照和高温下易降解[2]。Betanin具有抗炎和抗氧化活性，还具有神经保护作用[3]。
在体外，Betanin（10-80µM）处理人类慢性粒细胞白血病细胞系K562细胞12-48h，剂量和时间依赖性地抑制了细胞活力，诱导了细胞凋亡，诱导了细胞色素c从线粒体释放到细胞溶胶中，降低了膜电位[4]。Betanin（15μM）处理THP-1细胞24h，显著诱导了细胞中 IL-1β 、 IL-8和 IL-10 mRNA的表达[5]。
在体内，Betanin（100mg/kg）通过口服治疗神经退行性疾病模型小鼠2周，具有显著的抗焦虑作用，预防了海马CA1变性和胆碱乙酰转移酶（ChAT）活性改变，改善了空间学习和记忆障碍[6]。Betanin（20mg/kg）通过口服治疗高脂血症饮食诱导的氧化应激大鼠20天，降低了肝脏丙二醛含量，增加了超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性，逆转了肝组织损伤[7]。
| Cell experiment [1]: | |
Cell lines | K562 cells |
Preparation Method | Cells were placed in a 96-well plate and incubated with or without Betanin (10, 20, 40, 80µM) for 12, 24, and 48h. Cell viability was determined by MTT assay. |
Reaction Conditions | 10, 20, 40, 80µM; 12, 24, 48h |
Applications | Betanin inhibits cell viability in a dose- and time-dependent manner. |
| Animal experiment [2]: | |
Animal models | ICR mice |
Preparation Method | Forty male ICR mice were randomly divided into four groups: Sham-veh, TMT-veh, TMT-Bet50 and TMT-Bet100. In the TMT groups, neurodegeneration was induced with a one-time intraperitoneal injection of 2.6mg/kg TMT. Betanin-treated groups (Bet) were given oral doses of 50 or 100mg/kg dissolved in normal saline solution. Administrations were started 24h prior to TMT injection and continued for 2 weeks. Anxious behavior and spatial cognition were evaluated, respectively. After behavioral tests, brain oxidative status, hippocampal histology and choline acetyltransferase (ChAT) activity were evaluated. |
Dosage form | 50、100mg/kg for 2 weeks; p.o. |
Applications | Betanin 100mg/kg exhibited significant anxiolytic effect, preventive effect on CA1 degeneration and CA1 ChAT activity alteration as well as improvement of spatial learning and memory deficits. |
References: | |
| Cas No. | 7659-95-2 | SDF | |
| 别名 | 甜菜红 | ||
| Canonical SMILES | OC([C@@H]1CC(/C=C/N2C3=CC(O)=C(O[C@@H]4O[C@@H]([C@@H](O)[C@H](O)[C@H]4O)CO)C=C3C[C@H]2C(O)=O)=CC(C(O)=O)=N1)=O | ||
| 分子式 | C24H26N2O13 | 分子量 | 550.47 |
| 溶解度 | Water : 150 mg/mL (272.49 mM) | 储存条件 | Store at -20°C, protect from light, stored under nitrogen |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8166 mL | 9.0831 mL | 18.1663 mL |
| 5 mM | 0.3633 mL | 1.8166 mL | 3.6333 mL |
| 10 mM | 0.1817 mL | 0.9083 mL | 1.8166 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >95.00%
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