Bethanechol-d6 (chloride)
(Synonyms: Carbamyl-β-methylcholine-d6 chloride) 目录号 : GC48928
An internal standard for the quantification of bethanechol
Sample solution is provided at 25 µL, 10mM.
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Bethanechol-d6 is intended for use as an internal standard for the quantification of bethanechol by GC- or LC-MS. Bethanechol is an agonist of muscarinic acetylcholine receptors with IC50 values of 1,837, 25, 631, 317, and 393 µM for M1-5, respectively, in a radioligand binding assay using CHO cells expressing the human receptors.1 It inhibits M2-mediated increases in cyclic AMP induced by isoproterenol in isolated guinea pig small intestine (IC50 = 127 µM).2 Bethanechol increases basal tone of isolated porcine intravesical ureter (EC50 = 4.27 µM).3 It also induces fluid secretion in the ileum, duodenum, and jejunum of anesthetized rats when administered at a dose of 60 µg/kg.4 Formulations containing bethanechol have been used to increase urination and improve smooth muscle tone in the gastrointestinal tract.
1.Richards, M.H., and van Giersbergen, P.L.Human muscarinic receptors expressed in A9L and CHO cells: Activation by full and partial agonistsBr. J. Pharmacol.114(6)1241-1249(1995) 2.Okamoto, H., Prestwich, S.A., Asai, S., et al.Muscarinic agonist potencies at three different effector systems linked to the M2 or M3 receptor in longitudinal smooth muscle of guinea-pig small intestineBr. J. Pharmacol.135(7)1765-1775(2002) 3.HernÁndez, M., SÍmonsen, U., Prieto, D., et al.Different muscarinic receptor subtypes mediating the phasic activity and basal tone of pig isolated intravesical ureterBr. J. Pharmacol.110(4)1413-1420(1993) 4.Young, A., and Levin, R.J.Intestinal hypersecretion of the refed starved rat: A model for alimentary diarrhoeaGut33(8)1050-1056(1992)
| Cas No. | SDF | ||
| 别名 | Carbamyl-β-methylcholine-d6 chloride | ||
| Canonical SMILES | CC(OC(N)=O)C[N+](C([2H])([2H])[2H])(C)C([2H])([2H])[2H].[Cl-] | ||
| 分子式 | C7H11D6N2O2•Cl | 分子量 | 202.7 |
| 溶解度 | DMSO: soluble,Water: soluble | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 4.9334 mL | 24.667 mL | 49.334 mL |
| 5 mM | 0.9867 mL | 4.9334 mL | 9.8668 mL |
| 10 mM | 0.4933 mL | 2.4667 mL | 4.9334 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet