Betulinic Acid methyl ester
(Synonyms: 桦木酸甲酯,Methyl Betulinate) 目录号 : GC46099
A triterpenoid with diverse biological activities
Cas No.:2259-06-5
Sample solution is provided at 25 µL, 10mM.
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Betulinic acid methyl ester is a lupane-type triterpenoid and an esterified version of betulinic acid that has been found in Helicteres hirsuta and has diverse biological activities.[1],[2],[3],[4],[5] It inhibits HIV-1 reverse transcriptase (IC50 = 11 μM).[1] Betulinic acid methyl ester is active against L. braziliensis and T. cruzi (IC50s = 69.9 and 93.3 μM, respectively).[2] It induces melanogenesis in (EC50 = 2.5 μM) and inhibits the growth of B16 2F2 melanoma cells in vitro (IC50 = 4.9 μM).[3] Betulinic acid methyl ester is cytotoxic to SK-LU-1, HepG2, HeLa, SK-MEL-2, and AGS cells (IC50s = 60.84, 77.43, 80.17, 66.17, and 69.94 μg/ml, respectively).[4]
Reference:
[1]. Akihisa, T., Ogihara, J., Kato, J., et al. Inhibitory effects of triterpenoids and sterols on human immunodeficiency virus-1 reverse transcriptase. Lipids 36(5), 507-512 (2001).
[2]. Domínguez-Carmona, D.B., Escalante-Erosa, F., García-Sosa, K., et al. Antiprotozoal activity of betulinic acid derivatives. Phytomedicine 17(5), 379-382 (2010).
[3]. Hata, K., Hori, K., and Takahashi, S. Differentiation- and apoptosis-inducing activities by pentacyclic triterpenes on a mouse melanoma cell line. J. Nat. Prod. 65(5), 645-648 (2002).
[4]. Quang, D.N., Pham, C.T., Le, L.T.K., et al. Cytotoxic constituents from Helicteres hirsuta collected in Vietnam. Nat. Prod. Res. 34(4), 585-589 (2020).
[5]. Eyong, K.O., Bairy, G., Eno, A.A., et al. Triterpenoids from the stem bark of Vitellaria paradoxa (Sapotaceae) and derived esters exhibit cytotoxicity against a breast cancer cell line. Med. Chem. Res. 27(1), 268-277 (2017).
| Cas No. | 2259-06-5 | SDF | |
| 别名 | 桦木酸甲酯,Methyl Betulinate | ||
| 化学名 | (3β)-3-hydroxy-Lup-20(29)-en-28-oic acid, methyl ester | ||
| Canonical SMILES | CC([C@@H]1CC[C@]2(C(OC)=O)CC[C@@]3(C)[C@]4(C)CC[C@@]5([H])C(C)(C)[C@@H](O)CC[C@]5(C)[C@@]4([H])CC[C@]3([H])[C@]21[H])=C | ||
| 分子式 | C31H50O3 | 分子量 | 470.7 |
| 溶解度 | Soluble in methanol, ethanol, DMSO, DMF | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.1245 mL | 10.6225 mL | 21.245 mL |
| 5 mM | 0.4249 mL | 2.1245 mL | 4.249 mL |
| 10 mM | 0.2124 mL | 1.0622 mL | 2.1245 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet