Bikinin
(Synonyms: Abrasin) 目录号 : GC10752An inhibitor of Arabidopsis shaggy-related kinases
Cas No.:188011-69-0
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Kinase experiment: |
ASKs are expressed as GST-fusion proteins in Escherichia coli BL21. In vitro kinase assays are performed by incubating 50 ng GST-fusion protein, 10 μg myelin basic protein (MBP) as a substrate and 0.15 MBq γ-[32P]-ATP as co-substrate at 25°C for 30 min. The reaction buffer consists of 20 mM HEPES/KOH pH 7.4, 15 mM MgCl2, 5 mM EGTA, 1 mM dithiothreitol and 1 μM cold ATP. The reaction products are separated by SDS-PAGE and the amount of radioactivity incorporated into MBP quantified using an Amersham storage phosphor imager screen and a Biorad Molecular Imager FX[2]. |
References: [1]. Deng XG, et al. Role of brassinosteroid signaling in modulating Tobacco mosaic virus resistance in Nicotiana benthamiana. Sci Rep. 2016 Feb 3;6:20579. |
Bikini is a strong activator of Brassinosteroid (BR) signaling [1] and an inhibitor of glycogen synthase kinase 3 proteins (GSK3) [2].
BRs are a group of polyhydroxylated steroid hormones implicated in multiple developmental processes, including stem elongation, leaf expansion, vascular development, seed germination, and resistance to biotic and abiotic stresses. Genetic defects in biosynthesis or perception of BRs resulted in dwarfism, dark-green and curled leaves, reduced seed germination and fertility, and de-etiolation in the dark. [1]
Bikinin is the first nonsteroidal molecule to modulate the BR signaling cascade downstream of the BRI1 receptor. Bikinin directly targeted BIN2 and activated BR-dependent gene expression. Application of bikini partially restored the phenotype of the bri1 loss-of-function mutant and the bin2 gain-of-function mutant to wild-type. Bikinin clearly rescued the dwarfism of the hypocotyls and the petioles of the bin2 gain-of-function mutant. Bikinin are fully rescuing the BR-related phenotypes of this mutation while having no effect on the auxin-related phenotypes. [1]
Bikinin treatment also induced tracheary element differentiation in Nicotiana benthamiana leaf disks, this leaf-disk culture system may be applicable to different plant species. [2]
References:
[1]. De Rybel B, Audenaert D, Vert G et al. Chemical inhibition of a subset of Arabidopsis thaliana GSK3-like kinases activates brassinosteroid signaling. Chem Biol. 2009 Jun 26;16(6):594-604.
[2]. Kondo Y, Fujita T, Sugiyama M, Fukuda H. A Novel System for Xylem Cell Differentiation in Arabidopsis thaliana. Mol Plant. 2014 Dec 13. pii: S1674-2052(14)00026-4.
Cas No. | 188011-69-0 | SDF | |
别名 | Abrasin | ||
化学名 | 4-((5-bromopyridin-2-yl)amino)-4-oxobutanoic acid | ||
Canonical SMILES | BrC1=CN=C(NC(CCC(O)=O)=O)C=C1 | ||
分子式 | C9H9BrN2O3 | 分子量 | 273.08 |
溶解度 | ≥ 13.65mg/mL in DMSO | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 3.6619 mL | 18.3097 mL | 36.6193 mL |
5 mM | 0.7324 mL | 3.6619 mL | 7.3239 mL |
10 mM | 0.3662 mL | 1.831 mL | 3.6619 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
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