Biotin Hydrazide
(Synonyms: 生物素酰肼) 目录号 : GC12253
A biotinylation reagent
Cas No.:66640-86-6
Sample solution is provided at 25 µL, 10mM.
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Biotin hydrazide is a biotinyl derivative that can be used as a probe for the determination of protein carbonylation, which is a component of several diseases. Protein carbonylation, an irreversible post translational modification (PTM), is caused by attack of reactive oxygen species (ROS), numerous lipid oxidation products (such as α,β-unsaturated γ-hydroxyalkenals), or nonemzymatic glycation resulting in the loss of protein function. Biotin hydrazide is a preferred carbonyl-reactive probe for its direct reaction and chemistry and no requirement of additional catalysts or reducing agents. Biotin hydrazide exclusively and readily derivatizes carbonyl groups at pH 5.5, which facilitates its use to measure carbonylated proteins in biological samples.
Reference
[1].Kenneth Hensley, Kelly S. Williamson. Protein Carbonyl Determination Using Biotin Hydrazide. Methods in Biological Oxidative Stress. Methods in Pharmacology and Toxicology 2003, pp 195-199
[2].Madian AG, Regnier FE. Proteomic identification of carbonylated proteins and their oxidation sites. J Proteome Res. 2010;9(8):3766-3780.
| Biotinylation method [1]: | |
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Sample |
human umbilical cord HA |
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Preparation method |
Soluble in DMSO. Dissolve glycoprotein at a concentration of 1-5 mg/mL in cold 100mM Sodium Acetate, pH 5.5. |
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Reaction Conditions |
1 mM, overnight at 4℃ |
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Applications |
One hundred milligrams of human umbilical cord HA was dissolved in 0.1 M Mes, pH 5.0, to a final concentration of 1 mg/ml and allowed to dissolve for at least 24 h at 4℃ prior to the coupling of biotin. Biotin hydrazide was dissolved in DMSO as a stock solution of 100 mM and added to the HA solution to a final concentration of 1 mM. A stock solution of 1-ethyl-3-(3-dimethylamin-opropyl) carbidodiimide (EDAC) was prepared as a 100 mM stock solution in dH2O and added to the HA–biotin solution at a final concentration of 30 mM. This solution was left stirred overnight at 4℃. Unlinked biotin and EDAC were removed after the addition of 4M guanidine–HCl. The dialyzed, biotinylated HA (bHA) was aliquoted and stored at 0207C for up to several months. |
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References: [1]. Gregory I. Frost and Robert Stern. A Microtiter-Based Assay for Hyaluronidase Activity Not Requiring Specialized Reagents. ANALYTICAL BIOCHEMISTRY 251, 263–269 (1997). |
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| Cas No. | 66640-86-6 | SDF | |
| 别名 | 生物素酰肼 | ||
| 化学名 | 5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanehydrazide | ||
| Canonical SMILES | C1C2C(C(S1)CCCCC(=O)NN)NC(=O)N2 | ||
| 分子式 | C10H18N4O2S | 分子量 | 258.3 |
| 溶解度 | ≥ 12.9mg/mL in DMSO with gentle warming, ≥ 2.98 mg/mL in Water with ultrasonic | 储存条件 | Store at -20°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.8715 mL | 19.3573 mL | 38.7147 mL |
| 5 mM | 0.7743 mL | 3.8715 mL | 7.7429 mL |
| 10 mM | 0.3871 mL | 1.9357 mL | 3.8715 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet