Biotinylated Lycopersicon Esculentum (Tomato) Lectin (LEL, TL)
(Synonyms: 生物素化番茄凝集素; Biotin-LEL; Biotin-LEA) 目录号 : GC26645番茄凝集素(Tomato lectin,lycopersicon Esculentum Lectin(LEL))，是一种非常稳定的单亚基糖蛋白，约含有等量的蛋白质和碳水化合物。
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
Histochemistry:
1a. Staining procedure for paraffin sections: Deparaffinize and hydrate tissue sections through xylenes or other clearing agents and graded alcohol series and rinse for 5 minutes in tap water. If required, retrieve antigens using the Antigen Unmasking Solution.
1b. Staining procedure for frozen sections: Air dry sections. Immediately before staining, fix sections with acetone. Transfer slices to buffer. If endogenous enzyme activities are present, inactivate using appropriate methods.
2. Perform Streptavidin/Biotin blocking if required following kit instructions. Avidin in the Avidin/Biotin Blocking Kit is a glycoprotein containing terminal mannose residues. Use of the Avidin/Biotin Blocking Kit is not recommended especially when using mannose-specific lectins.
3. Block non-specific binding by incubating section with Carbo-Free™ Blocking Solution for 30 minutes at room temperature. Blot excess blocking solution from the sections.
4. Apply biotinylated lectin at approximately 2-20 µg/ml in PBS (10 mM sodium phosphate, 150 mM NaCl, pH 7.4) to the sections and incubate for 30 minutes at room temperature. Wash with TPBS (PBS + 0.05% Tween™ 20).
5. Prepare VECTASTAIN® ® ABC (peroxidase) or VECTASTAIN® ABC-AP (alkaline phosphatase) reagents according to the kit instructions. Apply to the sections and incubate for 30 minutes at room temperature. Wash with TPBS.
6. Apply an appropriate precipitating substrate for the enzyme system used in step 4. For peroxidase, ImmPACT™ DAB is recommended; for alkaline phosphatase, ImmPACT™ Vector® Red. Rinse in tap water.
7. Counterstain (optional), clear and mount. For galactose or GalNAc-specific lectins we recommend that sections be mounted in VECTASHIELD® HardFSet™ Mounting Medium or VECTASHIELD® HardFSet™ Mounting Medium with DAPI.
Biotinylated Lycopersicon Esculentum (Tomato) Lectin (LEL, TL) is a highly stable single subunit glycoprotein that contains approximately equal amounts of protein and carbohydrates. According to reports, its carbohydrate component is composed of 85% arabinose and 15% galactose, which may form polymeric substances in solution. Although tomato lectin shares some specificity with potato lectin, Datura lectin (DSL), and wheat germ lectin (WGA), it exhibits unique features in many aspects. Biotinylated Lycopersicon Esculentum (Tomato) Lectin (LEL, TL) can bind well to glycophorin and Tamm Horsfial glycoprotein, effectively binding and labeling the vascular endothelium of rodents.
番茄凝集素(Tomato lectin,lycopersicon Esculentum Lectin(LEL))，是一种非常稳定的单亚基糖蛋白，约含有等量的蛋白质和碳水化合物。据报道其糖类部分由85%阿拉伯糖和15%半乳糖组成，在溶液中可能形成多聚体聚合物。番茄凝集素虽然和土豆凝集素、曼陀罗凝集素(DSL)和麦胚凝集素(WGA)有部分相同的特异性，但在许多多面呈现出独特之处。番茄凝集素LEL能够很好地与血型糖蛋白(glycophorin)和Tamm-Horsfial糖蛋白结合，能够有效结合和标记啮齿类动物的血管内皮(vascular endothelium)。
| Cas No. | SDF | ||
| 别名 | 生物素化番茄凝集素; Biotin-LEL; Biotin-LEA | ||
| 分子式 | 分子量 | ||
| 溶解度 | 储存条件 | 2-8℃ | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。