Biperiden-d5
目录号 : GC49069
An internal standard for the quantification of biperiden
Cas No.:2938691-75-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Biperiden-d5 is intended for use as an internal standard for the quantification of biperiden by GC- or LC-MS. Biperiden is an antagonist of muscarinic acetylcholine receptors (mAChRs; IC50s = 0.48, 6.3, 3.9, 2.4, and 6.3 nM for M1-5 receptors, respectively).1 It increases spontaneous and electrically evoked dopamine release and electrically evoked acetylcholine (ACh) release from rabbit caudate nucleus slices preincubated with dopamine.2 Biperiden (10 µM) also inhibits NMDA-induced ACh release in rabbit caudate nucleus slices.3 Biperiden (0.01-1.0 mg/kg) reduces physostigmine-induced tremor in rats.4 Formulations containing biperiden have been used as an adjuvant treatment for Parkinson’s disease.
1.Bolden, C., Cusack, B., and Richelson, E.Antagonism by antimuscarinic and neuroleptic compounds at the five cloned human muscarinic cholinergic receptors expressed in Chinese hamster ovary cellsJ. Pharmacol. Exp. Ther.260(2)576-580(1992) 2.Jackisch, R., Huang, H.Y., Reimann, W., et al.Effects of the antiparkinsonian drug budipine on neurotransmitter release in central nervous system tissue in vitroJ. Pharmacol. Exp. Ther.264(2)889-898(1993) 3.Jackisch, R., Kruchen, A., Sauermann, W., et al.The antiparkinsonian drugs budipine and biperiden are use-dependent (uncompetitive) NMDA receptor antagonistsEur. J. Pharmacol.264(2)207-211(1994) 4.GothÓni, P., Lehtinen, M., and Fincke, M.Drugs for Parkinson’s disease reduce tremor induced by physostigmineNaunyn Schmiedebergs Arch. Pharmacol.323(3)205-210(1983)
| Cas No. | 2938691-75-7 | SDF | |
| Canonical SMILES | OC(CCN1CCCCC1)(C2CC3C=CC2C3)C4=C([2H])C([2H])=C([2H])C([2H])=C4[2H] | ||
| 分子式 | C21H24D5NO | 分子量 | 316.5 |
| 溶解度 | Acetonitrile: soluble,DMSO: soluble,Methanol: soluble | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.1596 mL | 15.7978 mL | 31.5956 mL |
| 5 mM | 0.6319 mL | 3.1596 mL | 6.3191 mL |
| 10 mM | 0.316 mL | 1.5798 mL | 3.1596 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
A novel method for the therapeutic drug monitoring of biperiden in plasma by GC-MS using salt-assisted liquid-liquid microextraction
Clin Chim Acta 2023 Mar 15;543:117322.PMID:37001688DOI:10.1016/j.cca.2023.117322
Biperiden is an anticholinergic agent with central effects. It is used in Parkinson's syndromes and in the treatment of extrapyramidal symptoms that occur with the use of various agents (neuroleptics, antipsychotics). It causes anticholinergic syndrome in high doses. For this reason, therapeutic drug monitoring of biperiden is important. This study, it was aimed to develop a validated GC-MS method for the therapeutic monitoring of biperiden in human plasma. Biperiden and internal standard Biperiden-d5 were extracted from plasma using the salt-assisted liquid-liquid extraction method. The method was validated according to the European Medicines Agency (EMA), Bioanalytical method validation guidelines. The lower limit of quantification of the developed method was chosen as 0.5 ng/mL. The calibration curve of biperiden for the method was validated between 0.5 and 15 ng/mL, showing correlation coefficients >0.99. In addition, the developed method was used for the therapeutic drug monitoring of biperiden in real patient plasma.